Core provides vision researchers with confocal, multiphoton, and macroview fluorescence microscopy together with histology services. Instrumentation includes Olympus FV1200 and FV1000 confocal systems, an Olympus FV1000-MPE multiphoton laser scanning microscope, an Olympus MVX10 macroview fluorescence system for bright-field and fluorescence imaging of flat mount tissues such as retinas and corneas, a Leica cryostat for sectioning, and Nikon E400 and E800 microscopes with dedicated review stations. Histology services include block cassettes, and special staining, and unstained sections.

Core provides vision researchers with in vivo imaging and functional analysis of visual system disease models, using instrumentation comparable to that used in clinical ophthalmology so that findings in animal models translate to human disease. Instrumentation includes Diagnosys electroretinography systems, Bioptigen SD optical coherence tomography, optokinetic tracking, Micron III and IV imaging systems with slit lamp and laser attachments, Zeiss operating microscopes with imaging, and specialized environmental housing including dark adaptation, dark rearing, and light damage. The core operates at two campus locations and also provides genotyping services for transgenic and mutant mouse and chick strains.

Core provides instrumentation and expertise for single-cell analysis in models of ocular development, degeneration, infection, and inflammation. Instrumentation includes a Cytek Aurora full-spectrum flow cytometer, a Cytek Aurora CS cell sorter, and a Luminex 200 multiplex suspension array system, together with an Agilent wound scratch tool, an Agilent Lionheart XL plate imager, and a CellZScope for real-time barrier physiology. Services include assisted and independent instrument use, training, panel and experiment design, sample preparation, and data analysis, with particular expertise in high-autofluorescence ocular tissues.

Core provides access to instrumentation in flow cytometry including the Cytek Aurora Evo analyzer and the Cytek Aurora CS. Services provided include guidance in flow cytometry sample preparation, panel design, instrument usage, cell sorting, troubleshooting, data analysis including proteomics, metabolomics, bulk RNA sequencing, single cell RNA sequencing, and spatial transcriptomics. Provides consultation in experimental design, training and guidance in data analysis, and assistance with troubleshooting.

Offers access to instrumentation, training and services for structure determination of macromolecular molecules using single crystal X-ray diffraction and/or cryo-EM Single Particle Analysis (SPA). Instrumentation is available for initial crystallization trials, optimization of crystallization, single crystal X-ray diffraction and data collection at synchrotron radiation facilities, as well as electron microscopy grid preparation for cryo-EM (SPA), screening and data collection using a Thermo Scientific Tundra Cryo-TEM and assistance for data collection at national laboratories.

Offers access to instrumentation, training, and services for protein expression, purification, and biophysical characterization. In addition to instrument access, PPCC personnel offer advice, hands-on training, education, and collaboration. Provides a range of protein purification and characterization equipment.

The Samuel Roberts Noble Microscopy Laboratory, the core imaging facility of the University of Oklahoma, is a central shared use facility offering access to instrumentation, training, and service using advanced light microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM).

Our mission is to provide effective and efficient access to core imaging and analytical characterization technologies. We welcome all OU-Norman, OUHSC, and OMRF student, faculty, and staff researchers at our centralized location the OU-Norman main campus. Clients from other universities, foundations and industry are welcome to use this equipment as well, and such work may be done on an invoice or contract basis.

In addition to instrument access, SRNML personnel offer advice, hands-on training, education, and research collaboration. The SRNML aims to promote, enable, and encourage cutting-edge education and research using the core’s instrumentation.

We have a range of sample preparation equipment available; our staff work with users and provide training. Please see our equipment and getting started pages for more information, and don’t hesitate to contact us.

The Oklahoma Medical Research Foundation Clinical Genomics Center offers high-throughput sequencing and genotyping services utilizing cutting edge Illumina and Xoford Nanopore systems. Our services are available to all outside academic and corporate research entities.

The mission of the Human Antibody Core Facility at OMRF is to:

•Successfully collaborate with OMRF, OUHSC and external investigators to define temporal human immune responses after vaccination and to generate human monoclonal antibodies to supplement their research.
•Develop novel methods to produce human monoclonal antibodies after acute infection and other immune events.
•Investigate new technologies for the characterization of human monoclonal antibodies produced by the core.
The Human Antibody Core Facility is one of the few laboratories in the world that produces fully-human, full-length, antigen-specific antibodies for use in studying human immune responses. The Core has achieved breakthroughs in antibody technology and has produced hundreds of high affinity protective antibodies to influenza, anthrax lethal toxin, and various S. pneumonia polysaccharides. The Core aims to support investigators by helping quantify the antibody secreting cell responses after vaccination and by generating human monoclonal antibodies to be characterized. Pathogen-specific human monoclonal antibodies are also available for licensing agreements and other forms of commercial development. The Core is expanding these services to aid additional clinical and basic science investigators inside and outside Oklahoma.