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All Facilities >> University of Freiburg >> UF-Lighthouse Core Facility
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Zentrum für Translationale Zellforschung
Breisacher Strasse 115
Freiburg,
79106Germanyhttps://www.uniklinik-freiburg.de/medizin1/forschung/lighthouse-core-facility.htmlcite this facility
Marie Follo
Lighthouse Core Facility (LCF) is a recognized core facility of the Medical Faculty of the University of Freiburg. It is also registered in the RIsources Database of the German Research Foundation (DFG) under the number RI_00416.
Lighthouse specializes in the areas of flow cytometry and cell sorting; light microscopy (confocal, widefield fluorescence, high content screening, lightsheet, slide scanning, highly multiplexed microscopy); and digital PCR / QPCR. It is located at the Medical Center - University of Freiburg.
The Lighthouse host institutions include the Dept. of Medicine I, the Center for Chronic Immunodeficiency (CCI), the CCCF / DKTK, and the Medical Faculty of the University of Freiburg.
Services are offerred outside of
Consulting is offerred outside of University of Freiburg
Last Updated: 05/06/2026
3D & 4D Image Processing Stations
Confocal/ Super Resolution Microscope
Fluorescence Microscope With Optical Sectioning Capability
BD Biosciences FACSAria III Cell Sorter
Instrument for cell sorting by BD Biosciences where positive air pressure forces sample cell through an optically gel-coupled cuvette . Hydrodynamic focusing guides particles in a single-file stream through the cuvette, where laser light intercepts the stream at the sample interrogation point.Fluorescence activated cell sorting instrument. Flow cytometer as high speed fixed alignment benchtop cell sorter. Enables multicolor analysis of up to 13 fluorescent markers and two scatter parameters at time. [Product Link]
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BD FACSAria Fusion Cell Sorter
Cell sorter has UV, Violet, Blue, Yellow/Green and Red lasers and can detect up to 18 colors. Can sort up to 4 populations simultaneously. Samples can be sorted into 12 x 75 mm FACS tubes or 1.5 ml Eppendorf tubes. Uses BD FACSDiva software to control setup, acquisition, and analysis of flow cytometry data from operator workstation. [Product Link]
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BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
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Beckman Coulter CytoFLEX LX Flow Cytometer
This model is equipped with four lasers (405, 488 561 and 638nm), and is capable of detecting FSC, SSC and 16 colors. [Product Link]
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Beckman Coulter CytoFLEX SRT Cell Sorter
Offers multiple configurations with the option to easily upgrade. Instrument can be configured to use up to four lasers and up to 15-color detection to identify subtle differences between cells. Core Facility’s CytoFLEX SRT is four-laser platform (405, 488, 561 and 638nm) instrument capable of detecting FSC, SSC and 15-colors and is able to sort up to four separate populations from the same sample, and deposit them in variety of tubes and plates. Operates under CytExpert SRT Software. [Product Link]
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Beckman Coulter Life Sciences MoFlo Astrios EQ Cell Sorter
High speed flow cytometry sorter. [Product Link]
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Roche Light Cycler 480 qPCR Real Time PCR System
Real time PCR device that allows use of additional thermal block cycler units 96 well and 384 well format. [Product Link]
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Sartorius IncuCyte S3 Live Cell Analysis System
System that automatically captures and analyzes images of living cells around-the-clock for days, weeks, or months, while cells remain undisturbed inside standard tissue culture incubator. Kinetic, image-based measurements. Used for cell monitoring and surveillance, cell health and viability, migration and invasion, phenotypic cell based assays. [Product Link]
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Sony Biotechnology Inc. SP6800 Spectral Cell Analyzer
The Sony SP6800 Spectral Analyzer is an analyzer that uses spectral technology to collect photons from 420nm to 800nm. Spectral technology also simplifies multicolor panel design, by eliminating bandpass filters and conventional compensation matrices while delivering data and simplifying visualization for the study of heterogeneous populations. Global standardization mode automatically sets the system to a master specification with a single click. This capability eliminates instrument variability from day to day and across multiple instruments for greater reliability. Advanced electronics and patented optical technologies bring simplicity to Spectral Analysis workflows. Sony’s patented Flowpoint™ core stability and tracking system and automated QC ensure the highest resolution possible of target populations. [Product Link]
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Sony ID7000 Spectral Cell Analyzer
Flow cytometer designed for comprehensive, high-parameter analysis of heterogeneous cell populations. It utilizes spectral technology supporting up to 7 lasers and 186 detectors to detect dim/rare populations and distinguish over 44 colors, making it ideal for advanced immunology and oncology research. [Product Link]
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Zeiss LSM 710 Confocal Inverted Microscope
LSM 710 is a confocal is an inverted microscope that enables confocal microscopy for a wide variety of applications. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. With the inverse Axio Observer from Carl Zeiss, LSM 710 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard. [Product Link]
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Zeiss LSM 880 with Airyscan Confocal Laser Scanning Microscope
Fully enclosed laser scanning confocal imaging microscopefor heating and environmental control with media perfusion capabilities and CO2 control. It can be used for Live Cell Imaging, FRET and Anisotropy, FRAP and FLIP, FCS, Photoactivation/ -conversion. [Product Link]
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Zeiss CellDiscoverer 7 with LSM 900 AiryScan2
Combined confocal/widefield system with LSM 900 scan head and AiryScan2. Fully integrated climate chamber for optimal live cell imaging.
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https://coremarketplace.org/?FacilityID=1806Institution
Institution ROR ID: https://ror.org/0245cg223Keywords:
USEDit, ABRF, flow cytometry, cell sorting, light microscopy, confocal, widefield, fluorescence, high content screening, lightsheet, slide scanning, highly multiplexed microscopy, digital PCR / QPCR,
Resource Type:
core facility, access service resource, service resource
Citation:
University of Freiburg Medical Center Lighthouse Core Facility (RRID:SCR_023785)
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Cryo-Electron Microscopy
Cryo-Electron Tomography
Sample Preparation
Cryo-EM
Stefan Steimle
Albertstr. 21
Freiburg, 79104 - Germany
RRID:SCR_025860
The CEF offers services related to cryo electron microscopy to all members of the University of Freiburg as well as external universities and research institutions.
Services include grid preparation, screening and data collection, as well as user training.
This facility provides services outside its institution
This facility provides consulting outside its institution
10/04/2024
3D &
4D Image Processing Stations
3D Printing
3D Visualization
Analysis Workstations
Clinical Imaging - Small Animal (X-ray, Ultrasound, microCAT, MRI)
Computed Tomography (CT)
Data Processing
Data Visualization
Functional Brain Imaging
Functional Magnetic Resonance Imaging (fMRI)
Image Aquisition
Magnetic Resonance Imaging (MRI)
Nuclear Magnetic Resonance (NMR)
Spectroscopy
Imaging (Cell, Molecular, PET, Translational)
Martin Buechert
Killianstr. 5a
Freiburg, 79106 - Germany
RRID:SCR_021926
MRDAC is an official core facility of the medical faculty of the University of Freiburg and is registered in the RIsources database of the German Research Foundation (DFG) under the number RI_00444.
The MR Development and Application Center origins in the Medical Physics group, Dept. of Radiology, University Medical Center Freiburg, one of the internationally leading groups in the field of methodological developments in Magnetic Resonance Imaging and Spectroscopy and its application in clinics and research.
MRDAC was established in 2001 to serve as a platform for collaborations with partners in industry and research according to industrial quality and performance standards. The incorporation of MRDAC as an institution of the University Medical Center Freiburg allows us to incorporate the expertise and excellence of clinicians and scientists from a wide range of fields into our projects and services. Thus, it serves as an interface between the scientific activities of the Medical Physics group and professional users in industry and academia.
The main branches of MRDAC include
- the development of novel MR sequences and methods and
- design, implementation, and execution of pharmaceutical MR studies.
In addition we offer services such as teaching, training, and consulting on-site as well as in Freiburg.
This facility provides services outside its institution
This facility provides consulting outside its institution
04/20/2023
Cell Culture
Cell Imaging
Confocal Microscopy
Light-sheet Microscopy
Live Cell Imaging
Multiphoton Microscopy
Optical Imaging
Super-resolution Microscopy
Roland Nitschke
University Freiburg Life Imaging Center
Habsburgerstr. 49
Freiburg, 79104 - Germany
RRID:SCR_020968
The Life Imaging Center (LIC) is a central core facility of the Albert-Ludwigs-University Freiburg open to members of all Faculties and the Universitätsklinikum. Currently more than 280 active users from six Faculties (Biology, Medicine, Chemistry and Pharmacy, Environment and Natural Resources, Mathematics and Physics, and Engineering) use the LIC infrastructure. LIC users come from more than 90 workgroups and the combined usage time of the LIC microscopes in 2017 was about 10.350 hours. The LIC has approximately 60 - 80 new users per year and approximately the same number leaving the facility each year.
The Life Imaging Center (LIC) consists of 14 rooms in the Center for Integrative Signalling Analysis (CISA) with approximately 400 m² (9 microscopy rooms with 20 microscope set-ups (3D-STED, confocal FLIM, two 2-photon confocal microscopes, 4 conventional confocal microscopes, lightsheet microscope, 4 high-end widefield ratio-imaging set-ups, 4 fluorescence stereo microscopes, 3 special set-ups for screening). The LIC microscopes are hosted in Biosafety level S2 and S1 environments, connected to a large wet lab area and a cell culture room, both Biosafety level S2 classified. Additionally, the LIC offers a large computer lab with 12 high-end workstations for image processing and analysis. The LIC also covers two additional rooms (35 m²), hosting 3 LSM systems in a neighboring building of the Signalling Campus Freiburg (SCF).
This facility provides services outside its institution
This facility provides consulting outside its institution
04/19/2023
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Facility Details
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