Flow Cytometry is an essential tool that is widely used in cancer research. The Flow Cytometry Shared Resource provides equipment, training, and operating assistance for cell sorting and analysis. Facility staff oversees equipment maintenance, trains new users, and assists with assay development and operation of equipment. Outside users are welcome to contact the facility manager for appointments, rates, or more information about services.
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The benchtop SH800S cell sorter is a cell sorter that sorts a wide range of cell sizes for many applications using the 70-μm, 100-μm, and 130-μm microfluidic sorting chips. This chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective and easier to perform. System software supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools. The SH800S is for non-clinical research use only and is a Class 1 laser product. The SH800S is flexible and can support a variety of applications for individual and core labs. For applications in which cross-contamination is a concern, optional e-beam sterilized consumables can be used to replace the sample line and sorting chip. An optional class A2 Level II biosafety cabinet provides protection for personnel and products. The biosafety cabinet was custom designed for the SH800S and tested by The Baker Company to meet international standards. The optical design offers up to four collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection. [Product Link]