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All Facilities >> Molecular & Cellular Immunology Core Facility (Flow Cytometry)
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University of Hawaii
John A. Burns School of Medicine
651 Ilalo Street, BSB 330
Honolulu, HI 96813
United States of Americahttps://cobre.manoa.hawaii.edu/pceidr/mci/flow/Alexandra Gurary
Last Updated: 04/25/2022
As the sole resource for flow cytometry, cell sorting and state-of-the-art immunological services in the western-most IDeA state, the Molecular and Cellular Immunology Core, through COBRE support, has accelerated research productivity, in terms of publications and extramural funding. Emphasis has also been applied to developing new or customized immunological methods for COBRE Investigators and other Core users. In addition, regularly scheduled training sessions are held to enrich the educational and mentoring experience for COBRE Investigators and other faculty and students across the university and broader research community. By centralizing immunological services and resources to this Core, COBRE Investigators and other researchers will be more efficiently served and the use of expensive equipment will be maximized.
Core Services:
Flow Cytometry: Analysis and Sorting
Cell Counting, Size, and Viability
Multiplex Bead Assays: Luminex and CBA Platforms for DNA, Protein, and Antibodies Assays
Immunospot Plate Reading: ELISpot and FRNT
Cell Irradiation
Flow Cytometry Assays:
Cell Phenotyping
Cell Cycle with Live or Fixed Cells
Apoptosis
FRET
Calcium Flux
Phosphorylation
Intracellular Cytokine and Protein Detection
Fluorescent Protein Expression
Cell Population Isolation
Single Cell Sorting
Dye Dilution Assays
Proliferation
Training opportunities
Experienced staff will provide hands-on training to investigators in conducting IBC/IACUC/CHS approved MCI research protocols. Please refer to MCI training document to help you get started on the process for obtaining clearance to work in the JABSOM Molecular and Cellular Immunology Core Facility.
Services are offerred outside of University of Hawaii
Consulting is offerred outside of University of Hawaii
Assisted Reproductive Technologies (Rodent IVF, ICSI)
Magnetic Resonance Imaging (MRI)
Protocol Development/Clinical Trial Coordination
BD Biosciences FACSAria IIu High Speed Cell Sorter
The High Speed Cell Sorter's primary function is to examine complex populations of cells and yield pure populations of cells. [Product Link]
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Attune Nxt Nxt
2020 Attune NxT Flow Cytometer makes multiparametric flow cytometry available to both new and experienced researchers. [Product Link]
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Luminex® 100 or 200™ Flow Cytometry Analyzer System
The Luminex® 100 or 200™ System is a flow cytometry analyzer system for individual labs that performs up to 100 different tests in a single reaction volume and can use either magnetic beads or plain polystyrene beads. Luminex 100 or 200 offers higher sample throughput with a 96-well plate read time of only 45 minutes. [Product Link]
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The MCI Core is equipped with BD FACSAria, BD FACSCalibur, GuavaEAsyCytePlus, Attune NxT V6, Luminex 200, MagPix, Beckman-Coulter ViCell, CTL-ImmunoSpot® S6 Fluorescent Analyzer, PerkinElmer Victor 3 plate reader, Bio-Rad and BioTek ELx808 ELISA readers and washers, and Faxitron compact 43855D X-ray irradiation system.
No publications have been associated with this facility
Links
All the links listed resolve to this core listing
https://coremarketplace.org/?FacilityID=272Keywords:
USEDit, ABRF, flow cytometry, cell sorting, immunological services, COBRE
Resource Type:
Resource, service resource, core facility, access service resource
Citation:
University of Hawaii at Manoa Molecular and Cellular Immunology Core Facility (RRID:SCR_021344)
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