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All Facilities >> University of Massachusetts Amherst >> UMass-Amherst Light Microscopy Facility (Microscopy (Electron, Fluorescence, Optical))
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240 Thatcher Way
UMass, Amherst
Amherst, MA 01003
United Stateshttps://www.umass.edu/ials/light-microscopycite this facility
James Chambers
Last Updated: 05/19/2025
Located on the 5th floor in the Life Science Laboratories on the UMass Amherst campus, the Light Microscopy Facility provides powerful resources for imaging model organisms, tissue, cells, biomaterials, and artificial structures and houses state-of-the-art equipment including almost every light microscopy imaging modality presently available. Cell culture facilities are also available nearby as well as other routine items necessary for biological imaging and material imaging. This facility has been designated a Nikon Centers of Excellence, thus providing a unique opportunity for training, demonstration, instrument development, and research.
The Light Microscopy Facility accepts samples and will perform requested analysis for both on-campus users as well as off-campus academic and industrial partners. We excel in the use of telemicroscopy through the use of remote imaging sessions, remote control of all instruments, and remote data analysis sessions available to all users. Further, we provide training to all users that plan to conduct experimentation. Following an initial consultation in which experimental requirements, expected outcomes, and careful scrutiny of feasibility, training and/or access is arranged through the Director.
Services are offerred outside of University of Massachusetts Amherst
Consulting is offerred outside of University of Massachusetts Amherst
Nikon A1 Confocal Laser Microscope
Fully automated A1 offers standard paired galvanometers with high resolution scanning at up to 4096 x 4096 pixels. The A1R model incorporates a hybrid scanner system utilizing a high speed resonant galvanometer capable of acquisitions up to 240 frames per second. Both scanners can be used simultaneously for experiments requiring acquisition and photoactivation by concurrently scanning the specimen. This supports advanced research methods using photoactivation fluorescence proteins and facilitates high-speed, live-cell work with a huge array of new imaging strategies. A spectral imaging detector further enables the A1 and A1R models to obtain up to 32 discreet spectral bandwidths of data in one acquisition, with spectral unmixing capabilities. The total system is controlled through NIS-Elements C applications software, which also enables full control of the Nikon Ti-E research inverted microscope equipped with Nikon's Focus System (PFS), widefield CCD cameras and an array of hardware devices. [Product Link]
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Nikon A1R Confocal Laser Scanning Microscope
Fully automated A1 offers standard paired galvanometers with high resolution scanning at up to 4096 x 4096 pixels. The A1R model incorporates a hybrid scanner system utilizing a high speed resonant galvanometer capable of acquisitions up to 240 frames per second. Both scanners can be used simultaneously for experiments requiring acquisition and photoactivation by concurrently scanning the specimen. This supports advanced research methods using photoactivation fluorescence proteins and facilitates high-speed, live-cell work with a huge array of new imaging strategies. A spectral imaging detector further enables the A1 and A1R models to obtain up to 32 discreet spectral bandwidths of data in one acquisition, with spectral unmixing capabilities. The total system is controlled through NIS-Elements C applications software, which also enables full control of the Nikon Ti-E research inverted microscope equipped with Nikon's Focus System (PFS), widefield CCD cameras and an array of hardware devices. [Product Link]
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Nikon A1R MP Confocal Microscope
Nikon A1R MP is a multiphoton imaging system featuring a high resolution galvanometer scanner and a high speed resonant scanner that is capable of frame rates from 30 fps at 512 x 512 pixels to as fast as 420 fps in band scan mode. New 4 channel non-descanned detectors with higher sensitivity, reduced dark current and broad spectral range allow for real time unmixing of closely spaced probes for accurate and high-contrast spectral imaging. This is especially important in multiphoton microscopy because of the overlap of emission spectra of probes and autofluorescence, which is often unavoidable when using a single laser line. [Product Link]
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Nikon SMZ-1 Microscope
Nikon SMZ-1 and SMZ-1B zoom stereo microscopes offer legendary Nikon stereo performance. Developed to fit a variety of applications and OEM needs, these advanced-design stereo microscopes have the features and image quality you expect from Nikon. High-performance optics and a 6V/10W halogen illuminator deliver images with excellent contrast, resolution, and brightness. The SMZ-1 and SMZ-1B are human-engineered to allow long periods of operation without fatigue. Mechanical design is yet rugged for daily trouble-free operation in industrial, laboratory, and classroom environments. All controls are easy to use. Nikon's versatile modular approach allows the SMZ-1/1B to be easily adapted to various configurations such as stand, arm, or table mounting. Similarly, the illuminator can be fixed to the instrument stand, placed in a diascopic base, or attached externally for oblique lighting. The SMZ-1/1B use a Nikon-designed twin-lens objective system inclined at 12o. Only the center of the lenses is used to provide the sharpest three-dimensional image and enable the operator to work more accurately and efficiently for prolonged periods without eye strain. [Product Link]
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Nikon A1R-TIRF Confocal with TIRF
Nikon A1R-SIMe Confocal with Structured Illumination
Nikon SMZ18 stereoscope
Nikon TiE with HCA high content
Nikon X1 spinning disk
Nikon Crest V2 spinning disk
Arcturus Laser Capture Microdissection
Keyence BZ-800
Nikon TiU with Axopatch electrophysiology hardware/software
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https://coremarketplace.org/?FacilityID=1170Institution
Institution ROR ID: https://ror.org/0072zz521Keywords:
USEDit, imaging model organisms, tissue imaging, cells imaging, biomaterials imaging, light microscopy imaging, ABRF, ABRF
Resource Type:
access service resource, core facility, service resource
Citation:
University of Massachusetts Light Microscopy Core Facility (RRID:SCR_021148)
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240 Thatcher Rd
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This facility provides services outside its institution
This facility provides consulting outside its institution
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240 Thatcher Rd
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Amherst, MA 01003 - United States
RRID:SCR_022357
The facility provides expertise and access to the latest, well-maintained instrumentation that is crucial to the research community of the University of Massachusetts system, the Five College system, other academic institutions, and industrial partners. We are able to offer our capabilities to researchers and invite them to use this equipment either on their own or with the help of faculty who have expertise in each method.
This facility provides services outside its institution
This facility provides consulting outside its institution
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637 N Pleasant St
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RRID:SCR_017907
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NGS instrumentation includes Illumina MiSeq, Illumina NextSeq 500, and Fluidigm C1 Single-Cell Analysis System.
GRL also has additional instrumentation required for sample processing and DNA/RNA library preparation for NGS. Instrumentation includes - Diagenode Bioruptor Pico Sonicator, Agilent 2100 Bioanalyzer, Qubit 3.0 Fluorometer, BluePippin (Sage Science) DNA size selection system, FastPrep-24 5G Sample Homogenizer (MP Biomedicals), Stratagene Mx3005P qPCR system, Eppendorf EpMotion 5070 for automated liquid sample dispensing.
This facility provides services outside its institution
This facility provides consulting outside its institution
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RRID:SCR_023477
Core provides space, equipment, plant and animal cell cultures, media, supplies and training on recharge basis for both academic labs and corporate partners. Labs are equipped with laminar flow hoods, incubators, centrifuges, refrigerators/freezers, basic microscopes and automated cell counters. Offers technical expertise and hands on support for custom research projects. Services include Cell Line expansion and cryopreservation;Stem cell culture;Liquid nitrogen storage;Assay Development/optimization; CRISPR based gene editing and stable clone selection; Mycoplasma testing; Lentiviral packaging, titer, transduction;Molecular biology.
This facility does not provide services outside its institution
This facility does not consult outside its institution
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