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All Facilities >> University of Massachusetts Amherst >> UMass Amherst Mass Spectrometry Core Facility (Mass Spectrometry)

UMass Amherst Mass Spectrometry Core Facility (Mass Spectrometry)

Facility Details

About This Facility

Services and Equipment

Publications

Associations

Metadata

University of Massachusetts Amherst

240 Thatcher Rd

Amherst, MA 01003

United States of America

http://www.umass.edu/ials/mass-spectrometry

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Quicklinks:

https://coremarketplace.org/umass-massspec https://coremarketplace.org/RRID:SCR_019063

Facility LIMS Page

https://corum.umass.edu

Primary Contact:

Stephen Eyles

Last Updated: 10/28/2020

Facility RRID

RRID:SCR_019063

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Facility Grant Number

OD010645

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Facility Details

We offer instrument training and service analysis using a range of mass spectrometry techniques, including ICP-MS for elemental analysis, GC-MS, LC-MS, proteomics, metabolomics, MALDI-TOF and MALDI imaging, HDX-MS

Facility Policies

Services are offerred outside of University of Massachusetts Amherst

Consulting is offerred outside of University of Massachusetts Amherst

(To see more facility information, click the in the upper right)

Services offered:

HRAM Mass Spectrometry

Inductively Coupled Plasma Mass-Spectrometry

Mass Spectrometry

Facility Equipment

Thermo Scientific Orbitrap Fusion
Perkin Elmer NexION 350D ICP-MS
Bruker microflex LRF MALDI-TOF
Bruker MicroTOF II ESI-TOF
Bruker SolariX FTMS
Bruker Daltonics ultrafleXtreme MALDI-TOF/TOF
Waters Synapt G2Si w/ LEAP HDX
Waters Synapt G2
Waters Xevo TQD triple quadrupole
Agilent 7890 GC / 7000B TQ MS

(To see more facility information, click the in the upper right)

Publications

Publications associated with this facility (Click To View):

1.) Limpikirati PK, Zhao B, Pan X, Eyles SJ, Vachet RW (2020 Jun 3). Covalent Labeling/Mass Spectrometry of Monoclonal Antibodies with Diethylpyrocarbonate: Reaction Kinetics for Ensuring Protein Structural Integrity. Journal of the American Society for Mass Spectrometry, 31(6), 1223-1232. doi: 10.1021/jasms.0c00067. PMID: 32310649.

2.) Deol KK, Eyles SJ, Strieter ER (2020 May 6). Quantitative Middle-Down MS Analysis of Parkin-Mediated Ubiquitin Chain Assembly. Journal of the American Society for Mass Spectrometry, 31(5), 1132-1139. doi: 10.1021/jasms.0c00058. PMID: 32297515.

3.) Duan Q, Liu MJ, Kita D, Jordan SS, Yeh FJ, Yvon R, Carpenter H, Federico AN, Garcia-Valencia LE, Eyles SJ, Wang CS, Wu HM, Cheung AY (2020 Mar). FERONIA controls pectin- and nitric oxide-mediated male-female interaction. Nature, 579(7800), 561-566. doi: 10.1038/s41586-020-2106-2. PMID: 32214247.

4.) Bednarski DM, Lantz EE, Bobst CE, Eisenhut AR, Eyles SJ, Fey JP (2020 Feb). Sterilization of epidermal growth factor with supercritical carbon dioxide and peracetic acid; analysis of changes at the amino acid and protein level. Biochimica et biophysica acta. Proteins and proteomics, 1868(2), 140334. doi: 10.1038/s41586-020-2106-2. PMID: 31786473.

5.) Li X, Eyles SJ, Thompson LK (2019 Nov 1). Hydrogen exchange of chemoreceptors in functional complexes suggests protein stabilization mediates long-range allosteric coupling. The Journal of biological chemistry, 294(44), 16062-16079. doi: 10.1038/s41586-020-2106-2. PMID: 31506298.

6.) Martin CB, Chaplin VD, Eyles SJ, Knapp MJ (2019 Oct 1). Protein Flexibility of the α-Ketoglutarate-Dependent Oxygenase Factor-Inhibiting HIF-1: Implications for Substrate Binding, Catalysis, and Regulation. Biochemistry, 58(39), 4047-4057. doi: 10.1021/acs.biochem.9b00619. PMID: 31499004.

7.) Liang C, Savinov SN, Fejzo J, Eyles SJ, Chen J (2019 Oct 8). Modulation of Amyloid-β42 Conformation by Small Molecules Through Nonspecific Binding. Journal of chemical theory and computation, 15(10), 5169-5174. doi: 10.1021/acs.jctc.9b00599. PMID: 31476124.

8.) Guerra D, Truebridge I, Eyles SJ, Treffon P, Vierling E (2018). Direct Measurement of S-Nitrosothiols with an Orbitrap Fusion Mass Spectrometer: S-Nitrosoglutathione Reductase as a Model Protein. Methods in molecular biology (Clifton, N.J.), 1747(10), 143-160. doi: 10.1007/978-1-4939-7695-9_12. PMID: 29600457.

9.) He T, Gershenson A, Eyles SJ, Lee YJ, Liu WR, Wang J, Gao J, Roberts MF (2015 Jul 31). Fluorinated Aromatic Amino Acids Distinguish Cation-π Interactions from Membrane Insertion. The Journal of biological chemistry, 290(31), 19334-42. doi: 10.1007/978-1-4939-7695-9_12. PMID: 26092728.

10.) Koshy SS, Li X, Eyles SJ, Weis RM, Thompson LK (2014 Dec 16). Hydrogen exchange differences between chemoreceptor signaling complexes localize to functionally important subdomains. Biochemistry, 53(49), 7755-64. doi: 10.1021/bi500657v. PMID: 25420045.

11.) Koshy SS, Eyles SJ, Weis RM, Thompson LK (2013 Dec 10). Hydrogen exchange mass spectrometry of functional membrane-bound chemotaxis receptor complexes. Biochemistry, 52(49), 8833-42. doi: 10.1021/bi401261b. PMID: 24274333.

12.) Eyles SJ, Gierasch LM (2010 Feb 16). Nature Proceedings of the National Academy of Sciences of the United States of America, 107(7), 2727-8. doi: 10.1021/bi401261b. PMID: 20133678.

13.) Chen YH, Comeaux LM, Eyles SJ, Knapp MJ (2008 Oct 21). Auto-hydroxylation of FIH-1: an Fe(ii), alpha-ketoglutarate-dependent human hypoxia sensor. Chemical communications (Cambridge, England), 107(39), 4768-70. doi: 10.1039/b809099h. PMID: 18830487.

14.) Frimpong AK, Abzalimov RR, Eyles SJ, Kaltashov IA (2007 Jun 1). Gas-phase interference-free analysis of protein ion charge-state distributions: detection of small-scale conformational transitions accompanying pepsin inactivation. Analytical chemistry, 79(11), 4154-61. . PMID: 17477507.

15.) Xiao H, Kaltashov IA, Eyles SJ (2003 May). Indirect assessment of small hydrophobic ligand binding to a model protein using a combination of ESI MS and HDX/ESI MS. Journal of the American Society for Mass Spectrometry, 14(5), 506-15. . PMID: 12745220.

16.) Eyles SJ, Kaltashov IA (2004 Sep). Methods to study protein dynamics and folding by mass spectrometry. Methods (San Diego, Calif.), 34(1), 88-99. . PMID: 15283918.

17.) Matsunaga I, Bhatt A, Young DC, Cheng TY, Eyles SJ, Besra GS, Briken V, Porcelli SA, Costello CE, Jacobs WR Jr, Moody DB (2004 Dec 20). Mycobacterium tuberculosis pks12 produces a novel polyketide presented by CD1c to T cells. The Journal of experimental medicine, 200(12), 1559-69. . PMID: 15611286.

18.) Xiao H, Hoerner JK, Eyles SJ, Dobo A, Voigtman E, Mel (2005 Feb). Mapping protein energy landscapes with amide hydrogen exchange and mass spectrometry: I. A generalized model for a two-state protein and comparison with experiment. Protein science : a publication of the Protein Society, 14(2), 543-57. . PMID: 15659380.

19.) Kaltashov IA, Zhang M, Eyles SJ, Abzalimov RR (2006 Oct). Investigation of structure, dynamics and function of metalloproteins with electrospray ionization mass spectrometry. Analytical and bioanalytical chemistry, 386(3), 472-81. . PMID: 16932945.

20.) Liu T, Marcinko TM, Vachet RW (2020 Jul 1). Protein-Ligand Affinity Determinations Using Covalent Labeling-Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 31(7), 1544-1553. doi: 10.1021/jasms.0c00131. PMID: 32501685.

21.) Zhao B, Zhuang J, Xu M, Liu T, Limpikirati P, Thayumanavan S, Vachet RW (2020 May 5). Covalent Labeling with an α,β-Unsaturated Carbonyl Scaffold for Studying Protein Structure and Interactions by Mass Spectrometry. Analytical chemistry, 92(9), 6637-6644. doi: 10.1021/acs.analchem.0c00463. PMID: 32250591.

22.) Liu J, Li Y, Deol KK, Strieter ER (2019 Sep 6). Synthesis of Branched Triubiquitin Active-Site Directed Probes. Organic letters, 21(17), 6790-6794. doi: 10.1021/acs.orglett.9b02406. PMID: 31398045.

23.) Limpikirati P, Pan X, Vachet RW (2019 Jul 2). Covalent Labeling with Diethylpyrocarbonate: Sensitive to the Residue Microenvironment, Providing Improved Analysis of Protein Higher Order Structure by Mass Spectrometry. Analytical chemistry, 91(13), 8516-8523. doi: 10.1021/acs.analchem.9b01732. PMID: 31150223.

24.) Limpikirati P, Hale JE, Hazelbaker M, Huang Y, Jia Z, Yazdani M, Graban EM, Vaughan RC, Vachet RW (2019 Apr). Covalent labeling and mass spectrometry reveal subtle higher order structural changes for antibody therapeutics. mAbs, 11(3), 463-476. doi: 10.1080/19420862.2019.1565748. PMID: 30636503.

25.) Chang LH, Strieter ER (2018 Sep 21). Reprogramming a Deubiquitinase into a Transamidase. ACS chemical biology, 13(9), 2808-2818. doi: 10.1021/acschembio.8b00759. PMID: 30137960.

26.) Jansen LE, Amer LD, Chen EY, Nguyen TV, Saleh LS, Emrick T, Liu WF, Bryant SJ, Peyton SR (2018 Jul 9). Zwitterionic PEG-PC Hydrogels Modulate the Foreign Body Response in a Modulus-Dependent Manner. Biomacromolecules, 19(7), 2880-2888. doi: 10.1021/acs.biomac.8b00444. PMID: 29698603.

27.) Crowe SO, Rana ASJB, Deol KK, Ge Y, Strieter ER (2017 Apr 18). Ubiquitin Chain Enrichment Middle-Down Mass Spectrometry Enables Characterization of Branched Ubiquitin Chains in Cellulo. Analytical chemistry, 89(8), 4428-4434. doi: 10.1021/acs.analchem.6b03675. PMID: 28291339.

28.) Borotto NB, Zhou Y, Hollingsworth SR, Hale JE, Graban EM, Vaughan RC, Vachet RW (2015 Oct 20). Investigating Therapeutic Protein Structure with Diethylpyrocarbonate Labeling and Mass Spectrometry. Analytical chemistry, 87(20), 10627-34. doi: 10.1021/acs.analchem.5b03180. PMID: 26399599.

(To see more facility information, click the in the upper right)

Associations

ABRF

Association of Biomolecular Resource Facilities

NERLSCD

Northeast Regional Life Science Core Directors

(To see more facility information, click the in the upper right)

Metadata

Links

All the links listed resolve to this core listing

https://coremarketplace.org/?FacilityID=1054
https://coremarketplace.org/umass-massspec
https://coremarketplace.org/RRID:SCR_019063

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

USEDit, mass spectrometry

Resource Type:

Resource, service resource, core facility, access service resource

Citation:

University of Massachusetts Amherst Mass Spectrometry Core Facility (RRID:SCR_019063)

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UMass Amherst Mass Spectrometry Core Facility (Mass Spectrometry)

Facility Details

About This Facility

Services and Equipment

Publications

Associations

Metadata

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