The staff is highly experienced in processing various biological samples, including tissues, cultured cells, bacteria, viruses and subcellular fractions for morphologic analysis by electron microscopy.
A wide variety of services are provided. EMRC provides full service (you "drop-off" your samples) or, depending on your interest and the nature of your work, technical support so that you can do your own work with our support. We offer you support in planning of experiments, training in sample preparation, use of equipment and data analysis. All necessary training and assistance is available.
Facility Policies
Services are offerred outside of Rockefeller University
Consulting is offerred outside of Rockefeller University
E-beam evaporator recommended for oxide and nitride materials. Designed to operate around the e-5 millibar vacuum range, EE-2 can also be used to deposit metals (non-toxic materials) if very low pressure is not a critical factor in your process. The water-cooled substrate holder can accommodate one wafer up to 3� diameter. EE-2 has a rotary-vane mechanical roughing pump and a turbo high-vacuum pump, and is a relatively low voltage (~3.8kV) electron beam evaporation deposition system with a 4-pocket, water-cooled rotating hearth. [Product Link]
Leica EM AFS2 is a freeze substitution platform that performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use. [Product Link]
Fully automated Leica VT1200 S vibrating blade microtome is designed to meet the highest sectioning demands for cutting fresh and fixed tissue in Neuroscience. To achieve sections of the highest quality that retain viable cells on the section surface, the vertical deflection of the blade can be measured by Leica's Vibrocheck measurement device and minimised with the blade holder. The instrument was designed in collaboration with Prof. Dr. Peter Jonas and his group at the Physiology Department, Freiburg Germany. [Product Link]
Laboratory microwave system that includes water recirculation system temperature restrictive temperature probe. It is also capable of variable wattage, programmable times, and four intermittent power settings. [Product Link]
core facility, service resource, access service resource
Citation:
Rockefeller University Electron Microscopy Resource Center Core Facility (RRID:SCR_017792)
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