West Virginia University
HSCN Room 2160
PO Box 9177
Morgantown, WV 26506-9177http://flowcore.hsc.wvu.edu/
Kathleen Brundage, Ph.D.
Last Updated: 04/07/2016
The WVU Flow Cytometry & Single Cell Core Facility (FCSCCF) provides instrumentation, training and scientific expertise for single cell analysis and sorting. It is equipped with three cytometers: a Becton-Dickenson (BD) FACSAria high-speed bench top sorter, a BD dual laser FACSCalibur and a BD LSRFortessa with 4 lasers, a high throughput sampling module, and a detector to analyze submicron-sized particles. For single cell separation, the facility has a Fluidigm C1 Single Cell Auto Prep System which isolates RNA/DNA/microRNA and generates cDNA (if needed) for downstream RNAseq, DNAseq or PCR from single cells. Other instrumentation in the WVU FSCSSF include a Miltenyi Biotec Otto Dissociator for disassociation of tissues into single cells or homogenization for molecular biology applications, a Miltenyi Biotec located in a tissue culture hood for sterile magnetic cell sorting and a Meso Scale Discovery Sector 2400 automated plate reader that detects multiple analytes by electrochemilumiscence. The facility has auxiliary laboratory space with appropriate equipment to train personnel and support the needs of researchers. The WVU FCSCCF has several software packages including FCS Express, CellQuest Pro and BD FACSDiva software for flow cytometry data acquisition and analysis. It also has two separate analysis work stations.
The facility is managed by the Director Kathleen Brundage, Ph.D who has training from the Bowdoin Flow Cytometry Course, from BD Biosciences, from Fluidigm and >20 years of experience with flow cytometry. She oversees the day-to-day operations of the facility, operates the FACSAria for cell sorting, provides necessary instrument and software training, and consults on experimental design and data analysis. The facility is house in an approximately 750 sq ft lab space located on the 2nd floor of the Robert C. Byrd Health Sciences Center North building.
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