The UWBC DNA Sequencing Facility was established to provide cost-effective, cutting edge sequencing resources available to the University of Wisconsin campus as well as colleagues at public and private institutions. We provide comprehensive, fee-for-service support for Illumina, PacBio, and Oxford Nanopore next generation sequencing projects, from project design and sample preparation (including DNA extraction) through downstream analysis in conjunction with the UWBC Bioinformatics Resource Facility. We are licensed providers of GBS (KeyGene) and we are Certified Service Providers of PacBio and Oxford Nanopore services. In addition, the Gene Expression Center performs RNA library prep, single cell RNA library prep, microarrays, and more. We can facilitate custom projects upon request. Our facility is committed to producing high-quality data, maintaining rapid turn-around times, and providing comprehensive support and resources.

Need help getting started or finding the right technology for your research? Have a question? Contact us for a consultation.

The Animal Models Core provides support for the generation of novel animal (mice, rats and swine) models using CRISPR or transgenic technology, cryopreservation, rederivation, and IVF recovery of rodent animal models.

The Biochemistry Optical Core (BOC) provides state-of-the-art instrumentation for light and fluorescence based microscopy, including epifluorescence, confocal, and super-resolution imaging. Established by a faculty-approved investment from the Department of Biochemistry Endowment, our goal is to serve the campus research community by providing access to and training on basic and advanced light microscopy techniques. Expertise and advice is available for the design of experiments involving these techniques.

Genome Editing & Animal Models (GEAM) provides services and expertise to generate new genome edited models, particularly mouse, rats, swine, and cell lines, as well as support the use of rodent models including cryopreservation, reservation, and assisted reproductive technologies.

The Advanced Genome Editing Laboratory (AGEL) provides expertise to cutting edge genome editing technology. This includes

A mass spectrometer facility that helps characterize the new and novel compounds synthesized at the University of Wisconsin-Madison.

The UWBC Gene Expression Center (GEC) is a fee-for-service facility that offers single cell RNA (scRNA), small RNA and bulk RNA library preparation and sequencing (Illumina, PacBio & Oxford Nanopore) services in collaboration with the UWBC DNA Sequencing Center. Illumina Beadarray Genotyping and Methylation services are also available on the iScan platform.

The Bioinformatics Resource Center (BRC) is a UW Biotechnology Center facility that provides bioinformatics support for high-throughput nucleic acid sequence data. We assist researchers with a wide variety of projects, including genome assembly, SNP detection, ChIP-Seq and expression profiling using RNA-Seq, RIP-seq, and small RNA analyses.

We provide basic computational support for analysis of the next-gen sequencing data so that it can be delivered to researchers in a usable form. We also provide project-specific advanced bioinformatic analysis for researchers with high throughput sequence data. BRC provides these services both for on-campus investigators as well as external customers, and for data generated at the UWBC or elsewhere.