Our core supports genome engineering using CRISPR technology. We provide services to generate knockouts, insertions, deletions, and point mutations in various organisms. Services for genome engineering include guideRNA design, homology-directed repair template design, production of modified mammalian cell lines, preparation of reagents for delivery to embryos, and mutation detection by targeted deep sequencing.
Pooled screening is also coordinated and supported by our team. We acquire, prepare, and validate pooled libraries as an Institute resource. In coordination of our pooled screen workflow, we work with other teams to track the progress of these projects. Once resulting cell populations from the screen are delivered, our team does genomic DNA purification, target amplification, and preparation for NGS.
We now offer a TAIL-PCR service to identify the location of random integration events. We have successfully identified locations in killifish, fly, zebrafish, and sea anemone.
Lastly, our team is responsible for maintaining and distributing the clone and vector collections for the Institute.