The SD BRIN/USD Proteomics Core Facility provides state-of-the-art proteomics services to researchers from South Dakota, as well as nationwide and internationally. Since 2002, the USD PCF has been providing proteomics analyses, training in the use of common instrumentation such as scanner, spot cutter, imaging software, technique, and search engine tools for protein identification. Moreover, help in resolving protocol issues, sample preparation, data analysis and visualization, grant application support and proteomics requirement to submit data to specialize per review journals. Our goal is to provide to the researchers with the capability to analyze and identify protein expression patterns in their experimental systems.

The Genomics Core Facility will continue to support DNA microarray analysis of differential gene expression, RNA and DNA analysis by the Agilent microfluidics chip reader, and real time PCR/RT-PCR. To maintain the relevance of the core, we believe that it is important to add new services to the core to enable examination of the functions of genes that have been shown to be differentially expressed by DNA microarray. To this end, the GCF is currently purchasing the Amaxa Nucleofector II transfection equipment. This technology allows transfection of DNA constructs, RNAi/siRNA/ shRNA, peptides, antibodies and other biomolecules into living eukaryotic cells. The Amaxa nucleofection system is optimized for transfection of cells that are otherwise difficult to transfect.