The SDSU Flow Cytometry and Live Imaging Core is a centralized, state-of-the-art shared resource facility housed in five dedicated laboratory rooms (878 sq. feet) within the North Life Sciences building. The Core is overseen by a Faculty Director and a Technical Director. All services are provided on a fee-for-service, hourly recharge basis and are available for BSL-1 and BSL-2 research activities. Major instrumentation includes a BD Biosciences FACSAria™ Fusion in a biosafety cabinet and FACSMelody™ cell sorting systems; two BD® LSR II flow cytometric analyzers; an Amnis® ImageStreamX Mark II imaging flow cytometer; a PerkinElmer In Vivo Imaging System (IVIS); and a MESO QuickPlex® Q 60MM electrochemiluminescence detection platform. The Core provides comprehensive instrumentation, expert technical support, and consultation in experimental design and data analysis that supports high-parameter cellular phenotyping (up to 18 colors), high-speed, multi-population sorting (up to four populations simultaneously) for enrichment or high-purity recovery, 96-well plate single-cell sorting for clonal expansion or downstream single-cell sequencing applications, multi-parameter quantitative imaging (up to six imaging channels), whole-organism fluorescence and bioluminescence imaging, and multiplex biomarker quantification.