The IUB Electron Microscopy Center (IUB-EMC) is a core facility located in Myers and Simon Hall on the IU-Bloomington campus. The IUB-EMC houses a large sample chamber 30 kV JSM 5800LV scanning electron microscope (SEM), a work-horse 100 kV JEM 1010 transmission electron microscope (TEM) and a more powerful 300 kV JEM 3200FS TEM. These microscopes were manufactured by the JEOL company. We have both standard and high-tilt Gatan cryo-holders for the JEOL 3200FS, which is equipped with an in-column energy filter, scan coils for scanning transmission electron microscopy (STEM) and detectors for bright-field and high-angle, annular dark-field imaging (BF- and HAADF-STEM) and energy dispersive X-ray spectroscopy (EDX). All these features make it an ideal instrument for both cryo-electron microscopy (cryoEM) and analytical electron microscopy (AEM). The facility also has the equipment necessary to prepare samples for these microscopes, including a new Wohlwend HPF Compact 2 high pressure freezer, a Leica AFS-2 freeze substitution device, various ultra-microtomes, both a Vitrobot Mk3 and an EMS manual plunge freezer, a Denton vacuum evaporator, a Balzars critical point dryer and a Polaron sputter coater. The IUB-EMC also has a close working relationship with the IUB campus computer center, facilitating storage and back-up of imaging data and the extensive computational infrastructure required for cryoEM.
The IUB-EMC is staffed by three Ph.D.-level scientists (staff director Gavin Murphy, Barry Stein and David Morgan), with over 70 years of combined experience using various EM techniques ranging from conventional sample preparation such as embedding, staining and sectioning through cryo-electron tomography (cryoET) and high resolution structural biology using cryoEM techniques. The staff both trains people to use equipment (fee-for-use work) and also collects and processes data for people (fee-for-service work). Users are encouraged to collect their own images, but feasibility studies and preliminary information can often be generated more rapidly by the staff. There is no charge for staff time used during consulting or equipment training. All users are charged an hourly rate for equipment usage. In the case of fee-for-service work, an additional hourly charge is added to cover staff time. We have close ties to the IUB Light Microscopy Imaging Center (LMIC) and are especially interested in talking with people who are using light microscopy, but have not even thought about the possibility of electron microscopy. Finally, the IUB-EMC is open to all academic and non-academic users.

Macromolecular X-ray Crystallography Facility provides all resources for Crystal Screening and Optimization, Data Collection and Structure Determination of macromolecules.

Provides access and training for confocal and super-resolution light microscopy systems with support for experimental design and data visualization.

Core uses mass spectrometry to characterize proteins as well as other biologically-derived molecules. Specializes in characterization of protein post-translational modifications, including glycosylation, phosphorylation, acetylation and persulfidation.Provides identification of protein-interactions including determination of protein-protein crosslinks and quantitative comparisons of complex samples.

Core provides basic husbandry, training, supplies, and specialized service support for medical research at IU School of Medicine.

Neuroimaging facility that enables cutting-edge neuroscience research and training using non-invasive imaging methodologies, including both MRI and EEG.

Provides study design and data analysis for health-related research, including grant or protocol development, sample size and power calculations, data management, data analysis, and scientific writing. Support can range from hourly support on a short-term project to including a biostatistician on a long-term grant collaboration.

CGB offers range of genomic services, including high-throughput DNA/RNA extraction, library preparation, next-generation sequencing, and bioinformatic analysis.

Mechanisms of Behavior Core offers the equipment, reagents, bench space, and expertise to answer questions related to the roles of genetics and hormones in relation to animal behavior.

Core offers flow cytometry analysis and cell sorting services. It houses FACS Aria II 5-laser, 14-color sorter, SH800 2-laser, 6-color user-run cell sorter, COPAS Select, Drosophila embryo and large particle sorter, LSRII, 4-laser, 14-color analyzer, MACSQuant VYB 3-laser, 8-color analyzer with 96-well plate option, as well as Moxi Z cell counter technology used for determining cell concentration and size distribution and Miltenyi QuadroMACS separator. Services include technician assisted sorting, as well as training for analyzers, SH800 and Moxi instruments. Help with experimental design is available.Facility maintains workstation with flow cytometry data analysis software, and has copy of this software as well as site license for use outside of facility.