University of Chicago

Contact Info:

Yuanyuan Zha, PhD

Kovler Viral Oncology Laboratories

910 E 58th St, 057

Chicago, IL 60637

https://him.uchicago.edu/

Grants and Identifiers:

RRID: RRID:SCR_017916

Instrumentation:

Other Equipment

Nanostring nCounterMax PerkinElmer Vectra Polaris quantitive pathology imaging station Anaerobic chamber RNAscope hybridization oven MACSQuant flow cytometer ELISA microplate reader ELISPOT 2-color microplate reader Countess counter Gentlemacs dissociator Tissue homogenizer

Services Provided:

Assays and Measurements

Flow Cytometric Analysis

PCR Arrays

Protein Extraction/Purification

Real-time qPCR

RNA analysis

SDS-PAGE

Western Blot

Relevant Publications:

1.) Wallace RP, Refvik KC, Antane JT, Brünggel K, Tremain AC, Raczy MR, Alpar AT, Nguyen M, Solanki A, Slezak AJ, Watkins EA, Lauterbach AL, Cao S, Wilson DS, Hubbell JA (2023 Dec 20). Synthetically mannosylated antigens induce antigen-specific humoral tolerance and reduce anti-drug antibody responses to immunogenic biologics Cell Reports Medicine, 5(1), 101345. . PMCID: 10829756.

2.) Gray LT, Raczy MM, Briquez PS, Marchell TM, Alpar AT, Wallace RP, Volpatti LR, Sasso MS, Cao S, Nguyen M, Mansurov A, Budina E, Watkins EA, Solanki A, Mitrousis N, Reda JW, Yu SS, Tremain AC, Wang R, Nicolaescu V, Furlong K, Dvorkin S, Manicassamy B, Randall G, Wilson DS, Kwissa M, Swartz MA, Hubbell JA (2021 Nov). Generation of potent cellular and humoral immunity against SARS-CoV-2 antigens via conjugation to a polymeric glyco-adjuvant. Biomaterials, 278(), 121159. . PMID: 34634664.

3.) Volpatti LR, Wallace RP, Cao S, Raczy MM, Wang R, Gray LT, Alpar AT, Briquez PS, Mitrousis N, Marchell TM, Sasso MS, Nguyen M, Mansurov A, Budina E, Solanki A, Watkins EA, Schnorenberg MR, Tremain AC, Reda JW, Nicolaescu V, Furlong K, Dvorkin S, Yu SS, Manicassamy B, LaBelle JL, Tirrell MV, Randall G, Kwissa M, Swartz MA, Hubbell JA (2021 Aug 25). Polymersomes Decorated with the SARS-CoV-2 Spike Protein Receptor-Binding Domain Elicit Robust Humoral and Cellular Immunity. ACS central science, 7(8), 1368-1380. doi: 10.1021/acscentsci.1c00596. PMID: 34466656.

4.) Volpatti LR, Wallace RP, Cao S, Raczy MM, Wang R, Gray LT, Alpar AT, Briquez PS, Mitrousis N, Marchell TM, Sasso MS, Nguyen M, Mansurov A, Budina E, Solanki A, Watkins EA, Schnorenberg MR, Tremain AC, Reda JW, Nicolaescu V, Furlong K, Dvorkin S, Yu SS, Manicassamy B, LaBelle JL, Tirrell MV, Randall G, Kwissa M, Swartz MA, Hubbell JA (2021 Apr 8). Polymersomes decorated with SARS-CoV-2 spike protein receptor binding domain elicit robust humoral and cellular immunity. bioRxiv : the preprint server for biology, (), . pii: 2021.04.08.438884. doi: 10.1101/2021.04.08.438884. PMID: 33851166.

Description:

The Human Immunologic Monitoring (HIM) facility serves as a specialized laboratory performing correlative assays for cancer-based clinical trials. We participate in project development to assist in assay selection and optimization prior to study initiation. We offer a pick-up service for specimens from patients at the U of C Hospital or outpatient clinics, and process the samples as indicated for the assays being performed. For some studies, isolation and freezing of peripheral blood lymphocytes for analysis at a later date is performed, while for others immediate staining of whole blood for specific cell surface markers of interest is carried out. In addition, our laboratory prepares clinical grade products, such as peptide vaccines, for administration into patients.