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Transmission Electron Microscopy (TEM) Core Facility

University of Miami

Contact Info:

Vania Almeida

1095 NW 14 terrace

Room 4-34

Miami, FL 33136

https://www.themiamiproject.org/research-resources/core-facilities/

Grants and Identifiers:

RRID: RRID:SCR_023492

Instrumentation:

AMT Digital camera

BioSpint 12

AMT Digital camera

NanoSprint 15

Leica Ultramicrotome

UC-7

Other Equipment

Phillips CM10 transmission electron microscope: -side-entry goniometer -maintained on service contract -resolution: 0.3 nm (point-to-point) -Accelerating voltage: 40, 60, 80, 100 kV -Magnification range: 18X to 450,000X The Phillips CM10 provides magnification from 18x to 450,000x and a resolution of 0.3nm. It is capable of accelerating voltages from 40-100kV, but operates at 80kV for most biological purposes. You can observe your specimen on the screen, or capture images on 4" x 4" film. The camera holds 56 sheets of film, and micrographs are printed with magnifications and sequential identification numbers. Negatives can be scanned for digital storage and manipulation on your own.

Services Provided:

Consultation

Electron Microscopy

Immuno Electron Microscopy

Microtomy/Electron

Microtomy/Light

Sample Preparation

Tissue Embed

Tissue Processing

Transmission Electron Microscopy

Relevant Publications:

Description:

The Transmission Electron Microscopy (TEM) Core facility is to provide the University of Miami, Miller School of Medicine academic and research community access to the conventional transmission electron microscope and associated sample processing services. The TEM Core has two JEOL JEM-1400 microscopes each equipped with an AMT digital camera available on a fee for service and first come first served basis to all student, faculty, researcher, or corporate entity that may need this specialized technique.