University of Cincinnati
Contact Info:
Ken Greis
3125 Eden Ave
Vontz Center
Cincinnati, OH 45267-0548
http://med.uc.edu/cancerbiology/research/proteomics
Grants and Identifiers:
RRID: RRID:SCR_025895
Instrumentation:
Other Equipment
(1) Sciex 4800 MALDI-TOF/TOF MS (2) Sciex 5600+ TripleTOF (quadrupole-tof ms) w/Eksigent NanoLC Ultra (3) Ettan IsoDalt 12 large format 2D gel system (4) ProteinPilot and Mascot multiprocessor search servers.
Services Provided:
2D Spectra Acquisition and Interpretation
Relevant Publications:
Description:
The UC Proteomics Laboratory (UCPL) is fully equipped with the protein separation and mass spectrometry equipment as well as the trained personnel to perform comparative analyses of complex protein mixtures and to identify the differentially-expressed proteins. Proteomics laboratory personnel also offer guidance to the research community regarding the feasibility of a given approach and/or advice on proteomics experimental design to enhance the probability of success. Please contact the lab director to set up a consultation. The research expertise in the UC Proteomics Laboratory provides the identification of differentially expressed proteins and the characterization of protein modifications (e.g. phospho, ubiquitin, glyco). Furthermore, various heavy isotope tagging (e.g. TMT) and label-free methods are used to both identify the proteins and quantify the changes in abundance. Additional research-oriented projects include methods to quantify global changes in modifications from cell and tissues, proximity labeling (e.g., APEX/bioID) profiling, and mass spectrometry-based methods for enzyme assays and high-throughput inhibitor screening.
