Sanford Burnham Prebys Medical Discovery Institute
Contact Info:
Yoav Altman
10901 N. Torrey Pines Rd
La Jolla, CA 92037
https://www.sbpdiscovery.org/biomedical-research/shared-resources/flow-cytometry
Grants and Identifiers:
RRID: RRID:SCR_014854
Other Citation IdentifiersP30 CA030199
Instrumentation:
BD FACSAria IIu High Speed Cell Sorter
Aria has four lasers, 405, 488, 561 and 633 nm, and can detect light in sixteen photomultiplier tubes. Its primary function is to examine complex populations of cells and yield pure populations of cells. The Aria can sort lymphocytes at a rate of approximately 15,000 events per second. For larger cells, the rate is much slower in order to optimize the passage of cells through the instrument. Cells from single populations can be sorted into 96-well plates. Alternatively, the Aria can sort into two 15-ml tubes or four 5-ml tubes. The Aria can purify samples that are simply positive and negative for a single fluorophore or as complex as nine-color samples with intricate gating schemes. The BD FACSAria IIu cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has three spatially separated lasers - 405 nm, 488 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a single fluorophore or as complex as multi-color samples with complex gating strategies. Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 ?m, 85 ?m and 100 ?m nozzles. If you need a yellow-green (561 nm) laser, check the BD FACSAria III instead. [Product Link]
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BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
Citation IDs: Center for AIDS Research award number 5P30AI050410
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(Equipment No Longer Active At this Facility)
BD LSRFortessa X-20 Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
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(Equipment No Longer Active At this Facility)
Amnis Amnis ImageStreamX MkII with 2 cameras
Imaging Flow Cytometer
(Equipment No Longer Active At this Facility)
BD FACSAria III High Speed Cell Sorter
electrostatic droplet cell sorter
Cytek Cytek Aurora
5-Laser Aurora Full Spectrum Analyzer
Citation IDs: S10OD032325
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Other Equipment
- BD FACSAria 12-color high-speed cell sorter with 407 nm, 488 nm, and 633 nm laser lines - BD FACSAriaIIu 14-color high-speed cell sorter with 405 nm, 488 nm, 561nm, and 640 nm lasers - BD LSRFortessa 14-color FACS analyzer with HTS plate loader for 96 or 384-well plates and four solid state lasers (405 nm, 488 nm, 561 nm, 640 nm) - Amnis ImageStream MkII Imaging Flow Cytometer with two cameras and 405 nm, 488 nm, and 647 nm lasers - BD FACSCanto 6-color FACS analyzer with 488 nm and 633 nm lasers - BD FACSCalibur 4-color analyzer with 488 nm and 635 nm lasers - Muse Cell Analyzer
Services Provided:
Data Analysis And Interpretation
High Dimensional Flow Cytometry Analysis
Shared Instrumentation Oversight & Maintenance
Single Particle Extracellular Vesicle Flow Cytometry
Relevant Publications:
Description:
The facility provides access to high-speed cell sorting, analytical flow and imaging flow cytometry on the SBP campus in La Jolla. Trained SBP investigators have 24-hr/day access to a variety of analytical flow cytometers available for independent use. Trained external users have access during business hours. Core staff provide technical expertise, hardware and software training, operate the facility\'s cell sorters and are available to assist with analysis experiments for those who prefer to have their samples run by an expert cytometrist. Scientists planning a flow cytometry experiment are encouraged to consult facility staff for assistance with protocols, fluorochrome selection or other aspects of experiment design.
