The CoreMarketplace: Molecular Virology Support Core (MVSC)

Oregon Health and Science University

505 NW 185th Avenue

Beaverton, OR 97006

http://www.ohsu.edu/xd/research/centers-institutes/onprc/research-services/research-support/virology.cfm

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Primary Contact:

Christoph Kahl

Last Updated: 07/02/2013

Facility Details

The Virology mission is to provide essential virology services, reagents, specialized expertise and training to investigators in order to advance a wide array of research, with a particular focus on non-human primates (NHP). These range from basic studies in viral pathogenesis and immunology all the way to viral vaccines, gene transfer, and gene therapy.

Specifically, the MVSC provides specialized expertise and infrastructure in five essential areas:

- Virus identification and quantification in clinical specimens

- Production of virus stocks, virus vectors, and viral antigen preparations

- Serologic assays to assess host antiviral immune responses

- Maintenance of cell stocks and sample processing

- Development of new reagents and standardized assays

Facility Policies

Services are offerred outside of Oregon Health and Science University

Consulting is offerred outside of Oregon Health and Science University

Services offered:

Assays and Measurements

Cell Culture

Data Analysis

Molecular Construct Services

Protein Extraction/Purification

Facility Equipment

J2-21M/E Centrifuge
Sanyo MCO-19AIC Safe Cell UV Incubators
Shaking incubator (Excella NB24)
PCR hood
Eppendorf microfuge
Microscope: Leica inverted microscope with CoolLED pE-100 (470 nm excitation) for fluorescent microscopy
Magnapure Compact for nucleic acid isolation (Roche)
Maxwell 16 for nucleic acid isolation (Promega)
GenoGrinder High Throughput Sample Homogenizer (SPEX)
Magnalyzer Tissue homogenizer (Roche)
Beckman L7-80 ultracentrifuge
Beckman Allegra 6R Table top centrifuge
Beckman Microfuge 22R
LN2 Dewer: CBS Isothermal V1500-AB Series
Sanyo -80°C Freezer
Biosafety Cabinets:
SterilGard (Class II, Type A/BS), The Baker Company
Labgard ES (Class II, Type A2), NuAire
Sonicator: Virsonic Virtis
Plate Washer: Biotek ELX405UCWS
Plate Reader (Fluorescence, Absorbance, Luminescence): Biotek Synergy Mx Mono-based Reader with Gen5 Secure Software
ABI 7500 quantitative PCR machine
ABI 9700 PCR cycler