Facility Details

This core provides investigators with multi-parameter flow cytometric analysis capabilities, training and service. Highly trained research staff are at hand, as are dedicated workstations with FloJo and Winlist software systems. In parallel, this core also provides services in cell separation procedures including fluorescence activated cell sorting via an upgraded FACS ARIA II system, and magnetic activated cell sorting systems (including large-scale AutoMACS procedures). Training and/or services in a number of cell-based assays also are provided. These include multiwell fluorimetry and the luminometry, Evos microscopy, Vicell analysis, cytospin preparations and processing, and colony forming assay scanning and quantitative analyses. ES cell components of this core include hands-on training and technical support for ES cell culture, manipulation and research (including transfection, transduction and differentiation). Select collections of mouse ES cells and NIH-approved human ES cell lines are maintained (with appropriate quality assurances). This core also maintains and provides appropriate feeder cell preparations for ES and IPS cell culture, and corresponding reagents. Also available are genomic DNA, RNA and protein preparations. In addition our ES core component maintains and provides fluorescent microscopes as well as a dedicated ES cell tissue culture facility (including tissue culture hoods, clinical centrifuges, CO2 incubators and LN2 storage).