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WSU Proteomics Core

Wayne State University

Contact Info:

Paul Stemmer

540 E. Canfield, Scott Hall, R. 2105

Detroit, MI 48201

https://research.wayne.edu/proteomics

Grants and Identifiers:

RRID: RRID:SCR_022879

Instrumentation:

Thermo Orbitrap Eclipse nanoLC/MS system

System includes Thermo Orbitrap Eclipse Tribid mass spectrometer and Waters M-Class Acquity nanoUPLC. This platform features sequential acquisition capabilities and multiple fragmentation types, enabling complex, in depth proteomic experiments. [Product Link]

RRID:SCR_022212

cite this instrument

Thermo Orbitrap Fusion nanoLC/MS system

System includes Thermo Orbitrap Fusion Tribrid mass spectrometer and Waters M-Class Acquity nanoUPLC. This platform features sequential acquisition capabilities and multiple fragmentation types, enabling complex, in depth proteomic experiments. [Product Link]

RRID:SCR_018702

cite this instrument

Thermo Orbitrap Eclipse

Mass spectrometer with MS3 capabilities for isobaric tag quantitative analysis

Thermo Orbitrap Fusion

Mass spectroemter capable of high depth profiling using multiplexing or data dependent analysis

Other Equipment

Orbitrap Fusion with ETD Orbitrap Q Exactive LTQ-XL with ETD ion trap LC-MS/MS TSQ Vantage tiplequad LC-MS/MS

Services Provided:

Data Analysis

Mass Spectrometry

Proteomics

SDS-PAGE

Western Blot

Relevant Publications:

Description:

Protein identification and proteome analysis using Orbitrao Eclipse, Fusion and Q Exactive LC-MS/MS platforms. Affinity selection for phosphopeptides with analysis using HCD, CID and ETD. Validation and quantitation of proteins using MRM with a TSQ Vantage triple quad.