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Benaroya Research Institute Human Immunophenotyping Core

Benaroya Research Institute

Contact Info:

Alice Wiedeman/Alice Long

1201 9th Ave

Seattle, WA 98101

https://benaroyaresearch.org/our-research/core-labs/human-immunophenotyping-core

Grants and Identifiers:

RRID: RRID:SCR_026355

Instrumentation:

Fluidigm CyTOF Helios Mass Cytometer

Mass cytometer that enables deep profiling of translational and clinical research samples across a range of cell surface and intracellular markers. [Product Link]

RRID:SCR_019916

cite this instrument

Services Provided:

Assay Development

Cell Culture

Cell Sorting

CyTOF

Cytokine Assays

ELISA

Experimental Design

FACS Cell Sorting

Flow Cytometric Analysis

Flow Cytometry

Flow Cytometry Data Analysis

High Dimensional Flow Cytometry Analysis

Immune Monitoring

Luminex Bead Based Cytokine Assays

Mass Cytometry

Multi-color Flow Cytometry

Research Design

Sample Preparation

Spectral Flow Cytometry

Training

Relevant Publications:

Description:

The Human Immunophenotyping (HIP) core at the Benaroya Research Institute (BRI) provides access to cutting-edge technologies and services enabling study of human immune-mediated diseases. We offer expertise in human immunology along with innovative assays and state-of-the-art technology. We specialize in robust, high-parameter single-cell assays utilizing conventional, spectral, and mass cytometry (CyTOF), which can be paired with sophisticated genomic and bioninformatic analyses through our connections with other outstanding BRI cores. Other services include, but are not limited to, cell sorting, short-term in vitro assays, DNA/RNA extraction, qPCR, and ELISA or multiplex protein analysis (Luminex). We tailor our support to meet the unique needs of each researcher. A full spectrum of services is available, from guidance on study design and training on new technologies through to execution, data analysis and interpretation. The HIP core serves investigators from BRI and Virginia Mason Medical Center (VMMC) as well as other institutions, including both non- and for-profit organizations.