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BIDMC-Flow Cytometry Core

Beth Israel Deaconess Medical Center

Contact Info:

John C Tigges

3 Blackfan Road

Boston, MA 02215

https://www.bidmc.org/research/core-facilities/flow-cytometry-core

Grants and Identifiers:

RRID: RRID:SCR_012305

Instrumentation:

Beckman Coulter CytoFLEX Flow Cytometer

Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]

RRID:SCR_019627

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Cytek Aurora Spectral Analyzer

Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]

RRID:SCR_019826

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(Equipment No Longer Active At this Facility)

Beckman Coulter CytoFLEX Nano

Sub-micron analyzer

Citation IDs: CytoFLEX Nano

cite this instrument

Beckman Coulter CytoFLEX Nano

Specifically designed for the analysis of nanoparticles, the CytoFLEX nano Flow Cytometer overcomes the limitations of traditional flow cytometry, giving researchers a sensitive, consistent and flexible solution that can help them advance their research. The CytoFLEX nano Flow Cytometer is a pivotal development in flow cytometry that allows users to analyze extracellular vesicles (EVs) at least as small as 40 nm* with ease while simultaneously offering up to 6 separate fluorescent channels of detection and 5 side scatter channels, to deliver full characterization and open additional avenues for your nanoparticle research.

(Equipment No Longer Active At this Facility)

Beckman Coulter CytoFLEX SRT

4 Laser, 14 paramter cell sorter

Beckman Coulter CytoFLEX SRT

CytoFLEX SRT Cell Sorter is a benchtop sorter. It is capable of meeting requirements for a wide range of sorting needs. And like the CytoFLEX Platform, it includes innovative technologies that simplify the setup and operation, empowering investigators to focus on the research questions. The Violet-Blue-Yellow Green-Red (V-B-Y-R) Series has 15 fluorescent detectors when fully activated. It can be purchased with as few as five with an option to activate additional lasers and detectors with an activation key. It is capable of complex sort logic with different combinations of sort settings on each of four streams, including the ability to catch aborts of one of the other streams. An optional Biosafety Cabinet including aerosol evacuation or a standalone Aerosol Evacuation Unit is available. Capable of complex sort logic, including 4-way sorting, Mixed Mode sorting, and the ability to catch aborts and preserve precious cells Setup is smart and simplified, using automation to establish and maintain the sort stream Remote support ready using BeckmanConnect for on demand access to our technical specialists, perform many routine maintenance procedures independently Explore more resources about the CytoFLEX SRT benchtop sorter here.

Becton Dickinson FACSAria Symphony S6

The BD FACSymphony™ S6 System adds cutting-edge technology to the performance of the BD FACSAria™ Fusion Cell Sorter and the innovative optics of the BD FACSymphony™ A5 Cell Analyzer to power your scientific discovery. Configurable up to 50 parameters to define phenotypically distinct subpopulations and isolate populations previously only available for analysis Flexibility to sort subpopulations up to 6 ways or into plates or slides

(Equipment No Longer Active At this Facility)

Becton Dickinson Symphony S6

Multiparametric, 6-way cell sorter

(Equipment No Longer Active At this Facility)

Nexcelom Celigo

3 parameter imaging cytometer

Revvity Celigo

Celigo is a plate-based benchtop brightfield and fluorescent imaging system designed for whole-well live-cell analysis and cell sample characterization. The Celigo system images and analyzes cells in various types of vessels including 6 – 1536 well plates, T25, T75 flasks, 10 cm dishes, and glass slides without disturbing their natural state. Individual cell level analysis is easily generated, providing cell level insights unlike ELISA or protein-based assays, and at a faster rate than flow cytometry.

Services Provided:

Exosomes Characterisation

FACS Cell Sorting

Flow Cytometric Analysis

Flow Cytometry Data Analysis

Flow Cytometry Training

Full Spectrum Flow Cytometry

High Dimensional Flow Cytometry Analysis

Imaging Flow Cytometry

NanoFACS

NanoFlow Cytometry Training Course

RNA Flow Cytometry

Single Particle Extracellular Vesicle Flow Cytometry

Spectral Flow Cytometry

Relevant Publications:

1.) Tran V, de Oliveira-Jr GP, Chidester S, Lu S, Pleet ML, Ivanov AR, Tigges J, Yang M, Jacobson S, Gonçalves MCB, Schmaier AA, Jones J, Ghiran IC (2024 Oct). Choice of blood collection methods influences extracellular vesicles counts and miRNA profiling. Journal of extracellular biology, 3(10), e70008. . ID: 39440167.


2.) Kim J, Xu S, Jung SR, Nguyen A, Cheng Y, Zhao M, Fujimoto BS, Nelson W, Schiro P, Franklin JL, Higginbotham JN, Coffey RJ, Shi M, Vojtech LN, Hladik F, Tewari M, Tigges J, Ghiran I, Jovanovic-Talisman T, Laurent LC, Das S, Gololobova O, Witwer KW, Xu T, Charest A, Jensen KVK, Raffai RL, Jones JC, Welsh JA, Nolan JP, Chiu DT (2024 Aug). Comparison of EV characterization by commercial high-sensitivity flow cytometers and a custom single-molecule flow cytometer. Journal of extracellular vesicles, 13(8), e12498. . ID: 39140467.


3.) Zimmerman AJ, de Oliveira GP Jr, Su X, Wood J, Fu Z, Pinckney B, Tigges J, Ghiran I, Ivanov AR (2024 Mar). Multimode chromatography-based techniques for high purity isolation of extracellular vesicles from human blood plasma. Journal of extracellular biology, 3(3), . . ID: 38751711.


4.) de Oliveira Junior GP, Welsh JA, Pinckney B, Palu CC, Lu S, Zimmerman A, Barbosa RH, Sahu P, Noshin M, Gummuluru S, Tigges J, Jones JC, Ivanov AR, Ghiran IC (2023 Nov). Human red blood cells release microvesicles with distinct sizes and protein composition that alter neutrophil phagocytosis. Journal of extracellular biology, 2(11), . . ID: 37942280.


5.) Arce JE, Welsh JA, Cook S, Tigges J, Ghiran I, Jones JC, Jackson A, Roth M, Milosavljevic A (2023 Jun 1). The NanoFlow Repository. Bioinformatics (Oxford, England), 39(6), . . ID: 37285317.


6.) Valkov N, Das A, Tucker NR, Li G, Salvador AM, Chaffin MD, Pereira De Oliveira Junior G, Kur I, Gokulnath P, Ziegler O, Yeri A, Lu S, Khamesra A, Xiao C, Rodosthenous R, Srinivasan S, Toxavidis V, Tigges J, Laurent LC, Momma S, Kitchen R, Ellinor P, Ghiran I, Das S (2021 Dec). SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart. Life science alliance, 4(12), . . ID: 34663679.


7.) Oliveira-Jr GP, Barbosa RH, Thompson L, Pinckney B, Murphy-Thornley M, Lu S, Jones J, Hansen CH, Tigges J, Wong WP, Ghiran IC (2021 Oct 1). Electrophoretic mobility shift as a molecular beacon-based readout for miRNA detection. Biosensors & bioelectronics, 189(), 113307. . ID: 34062334.


8.) Thompson L, Pinckney B, Lu S, Gregory M, Tigges J, Ghiran I (2021 May 17). Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation. Journal of visualized experiments : JoVE, (171), . doi: 10.3791/62550. ID: 34057455.


9.) Li J, Salvador AM, Li G, Valkov N, Ziegler O, Yeri A, Yang Xiao C, Meechoovet B, Alsop E, Rodosthenous RS, Kundu P, Huan T, Levy D, Tigges J, Pico AR, Ghiran I, Silverman MG, Meng X, Kitchen R, Xu J, Van Keuren-Jensen K, Shah R, Xiao J, Das S (2021 Jan 8). Mir-30d Regulates Cardiac Remodeling by Intracellular and Paracrine Signaling. Circulation research, 128(1), e1-e23. . ID: 33092465.


10.) Oliveira GP Jr, Zigon E, Rogers G, Davodian D, Lu S, Jovanovic-Talisman T, Jones J, Tigges J, Tyagi S, Ghiran IC (2020 Jan 24). Detection of Extracellular Vesicle RNA Using Molecular Beacons. iScience, 23(1), 100782. . ID: 31958756.


11.) Welsh JA, Van Der Pol E, Arkesteijn GJA, Bremer M, Brisson A, Coumans F, Dignat-George F, Duggan E, Ghiran I, Giebel B, Görgens A, Hendrix A, Lacroix R, Lannigan J, Libregts SFWM, Lozano-Andrés E, Morales-Kastresana A, Robert S, De Rond L, Tertel T, Tigges J, De Wever O, Yan X, Nieuwland R, Wauben MHM, Nolan JP, Jones JC (2020). MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments. Journal of extracellular vesicles, 9(1), 1713526. . ID: 32128070.


12.) Jones PS, Yekula A, Lansbury E, Small JL, Ayinon C, Mordecai S, Hochberg FH, Tigges J, Delcuze B, Charest A, Ghiran I, Balaj L, Carter BS (2019 Oct). Characterization of plasma-derived protoporphyrin-IX-positive extracellular vesicles following 5-ALA use in patients with malignant glioma. EBioMedicine, 48(), 23-35. . ID: 31628025.


13.) Tracy SA, Ahmed A, Tigges JC, Ericsson M, Pal AK, Zurakowski D, Fauza DO (2019 Jan). A comparison of clinically relevant sources of mesenchymal stem cell-derived exosomes: Bone marrow and amniotic fluid. Journal of pediatric surgery, 54(1), 86-90. doi: 10.1016/j.jpedsurg.2018.10.020. ID: 30361074.


14.) Morales-Kastresana A, Musich TA, Welsh JA, Telford W, Demberg T, Wood JCS, Bigos M, Ross CD, Kachynski A, Dean A, Felton EJ, Van Dyke J, Tigges J, Toxavidis V, Parks DR, Overton WR, Kesarwala AH, Freeman GJ, Rosner A, Perfetto SP, Pasquet L, Terabe M, McKinnon K, Kapoor V, Trepel JB, Puri A, Kobayashi H, Yung B, Chen X, Guion P, Choyke P, Knox SJ, Ghiran I, Robert-Guroff M, Berzofsky JA, Jones JC (2019). High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer. Journal of extracellular vesicles, 8(1), 1597603. . ID: 31258878.


15.) Zigon ES, Purseglove SM, Toxavidis V, Rice W, Tigges J, Chan LL (2018 Oct). A rapid single cell sorting verification method using plate-based image cytometry. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 93(10), 1060-1065. doi: 10.1002/cyto.a.23520. ID: 30071132.


16.) Bei Y, Xu T, Lv D, Yu P, Xu J, Che L, Das A, Tigges J, Toxavidis V, Ghiran I, Shah R, Li Y, Zhang Y, Das S, Xiao J (2017 Jul). Exercise-induced circulating extracellular vesicles protect against cardiac ischemia-reperfusion injury. Basic research in cardiology, 112(4), 38. . ID: 28534118.


17.) Kuo WP, Tigges JC, Toxavidis V, Ghiran I (2017). Red Blood Cells: A Source of Extracellular Vesicles. Methods in molecular biology (Clifton, N.J.), 1660(), 15-22. doi: 10.1007/978-1-4939-7253-1_2. ID: 28828644.


18.) Camacho V, Toxavidis V, Tigges JC (2017). Characterization of Extracellular Vesicles by Flow Cytometry. Methods in molecular biology (Clifton, N.J.), 1660(), 175-190. doi: 10.1007/978-1-4939-7253-1_14. ID: 28828656.


19.) Akuthota P, Carmo LA, Bonjour K, Murphy RO, Silva TP, Gamalier JP, Capron KL, Tigges J, Toxavidis V, Camacho V, Ghiran I, Ueki S, Weller PF, Melo RC (2016). Extracellular Microvesicle Production by Human Eosinophils Activated by "Inflammatory" Stimuli. Frontiers in cell and developmental biology, 4(), 117. . ID: 27833910.


Description:

Committed to meet all of the flow cytometry needs for the BIDMC and the external research community, the Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cell sorting. It is continuously expanding with the newest software and machinery for both sorting and analysis, with the capability of up to 48 fluorescent parameters.