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Advanced Cell Analysis Service Center

University of Cincinnati

Contact Info:

Birgit Ehmer

3125 Eden Ave

Cincinnati, OH 45219

https://med.uc.edu/research/core/Index/36/Facility/

Grants and Identifiers:

RRID: RRID:SCR_025797

Instrumentation:

BD LSRFortessa Fortessa Flow Cytometer

BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]

RRID:SCR_019601

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(Equipment No Longer Active At this Facility)

Zeiss Axioplan 2 imaginig system

Upright microscopy imaging platform for complex research that is optimized for light transmission and extremely high contrast fluorescence imaging. This complete system comes fully configured for best performance, range and functionality. [Product Link]

RRID:SCR_020918

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Zeiss LSM 710 Confocal Inverted Microscope

LSM 710 is a confocal is an inverted microscope that enables confocal microscopy for a wide variety of applications. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. With the inverse Axio Observer from Carl Zeiss, LSM 710 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard. [Product Link]

RRID:SCR_018063

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BD BD FACS Aria III

Cell sorter for 2 or 4 way sort or sorting onto plates.

Nikon Nikon SIMe

Super-Resolution Microscope based on structural illumination

Sartoris Incucyte Zoom

Microscope inside an incubator suitable for long-term time lapses

Zeiss Axiovert100TV

inverted widefield microscope with B&W camera

Zeiss Zeiss Axioplan Imaging 2e

upright microscope with color and B&W camera

Services Provided:

Analysis Workstations

Brightfield Microscopy

Cell Imaging

Cell Sorting

Confocal Microscopy

Consultations

Data Visualization

FACS Cell Sorting

Flow Cytometry

Fluorescence Microscope With Optical Sectioning Capability

Image Aquisition

Image Processing And Analysis

Incucyte

Live Cell Imaging

Microscopy

Stereomicroscopy

Super-resolution Microscopy

Training

Widefield Microscopy

Relevant Publications:

Description:

The Department of Cancer Biology houses the Advanced Cell Analysis Service Center (ACASC) which offers access to a variety of microscopes and flow cytometry equipment for researchers. Services offered: Upright and inverted widefield microscopy Confocal Microscopy (Zeiss LSM 710) Super-Resolution Microscopy (Nikon SIMe) Live Cell Microscopy (Incucylte) Flow Cytometry (BD LSR Fortessa) Cell Sorting (BD Aria III)