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Stable Isotope & Metabolomics Core

Albert Einstein College of Medicine

1301 Morris Park Ave

Price Bldg., Room 368

Bronx, NY 10461

United States of America

http://www.einstein.yu.edu/diabetes/page.aspx?id=1566

Primary Contact:

Gustavo Palacios/ PhD

Last Updated: More than One Year ago

Facility Details

The newly established Einstein Stable Isotope & Metabolomics Core uses stable isotope flux and metabolite profiling to help formulate and test hypotheses about the metabolic consequences of various changes in gene expression and protein function, in order to guide further integrative systems biology analyses of the underlying mechanisms in diabetes, insulin resistance, obesity, and diabetic complications.

The Stable Isotope & Metabolomics Core’s evaluation framework complements the analyses performed in the Animal Physiology Core, such as measurements of whole body carbohydrate/fatty acid oxidation, energy expenditure, tissue-specific insulin sensitivity, glucose, protein and fatty acid metabolism.

Objectives

1.In vivo stable isotope substrate flux assays to determine rates of protein synthesis, lipogenesis, peripheral glucose disposal, hepatic glucose recycling, glucose-glycerol cycling and Cori cycling.
2.In vitro assessments of glycolysis (extracellular acidification rates) and mitochondrial oxygen consumption (mitochondrial respiration) in tissue explants, primary isolated and tissue culture cells using Seahorse Biosciences Flux Analyzers, as well as more comprehensive stable isotope flux assessments.
3.Assessments of plasma and tissue metabolite profiles for diagnosis/characterization of physiological and pathophysiological states. These include key metabolites in the glycolytic, gluconeogenic, pentose phosphate, and tricarboxylic (TCA) cycle pathways, fatty acid oxidation and complex lipids, such as ceramides, diacylgylcerols (DAGs), triacylglycerols (TAGs), and phospholipids.

Services

The metabolic assessments offered by the Stable Isotope & Metabolomics Core center on the application of 3 interacting methodologies, the Seahorse Biosciences Flux Analyzer, gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS).

Services are divided into four components:

1.Stable isotope substrate flux dynamics (mainly GC/MS based)
2.Metabolite quantification (GC/MS and/or LC/MS based)
3.Cellular/Organelle mitochondrial respiration and glycolysis rates (Seahorse Biosciences Flux Analyzer)

Other Facility Information

Prioritization

Use of this Core is first given to investigators engaged in diabetes-related research at Albert Einstein College of Medicine and its affiliated Institutions. In addition, the Core will try to acept requests from investigators at other institutions where such services are not readily available. The Core generally supports projects funded by peer-reviewed grants, generally from the NIH, ADA, or JDRF.

Facility Policies

Services are offerred outside of Albert Einstein College of Medicine