Oregon Health & Science University

Contact Info:

Robert Searles

L581

3181 Sam Jackson Park Rd

Portland, OR 97239

https://www.ohsu.edu/integrated-genomics-laboratory

Grants and Identifiers:

RRID: RRID:SCR_022651

Instrumentation:

Other Equipment

See www.ohsu.edu/gpsr, www.ohsu.edu/mpssr, and www.ohsu.edu/dnaseq for a listing of major equipment.

Services Provided:

10x Genomics

Cell Line Authentication

CITE-seq

Consultations

Copy Number Variation (CNV)

DNA Analysis

DNA Isolation

Genomics

Genotyping

Nanostring NCounter

Nucleic Acid Extraction

Outreach

PCR Arrays

Real-time qPCR

RNA analysis

RNA Integrity

RNA Isolation

RNAseq

Sequencing - DNA Sequencing

Sequencing - Next Generation Sequencing (NGS)

Single-Cell Analysis

Single-cell Sequencing

TaqMan Arrays

Training

Whole Exome Sequencing

Whole Genome Sequencing

Relevant Publications:

1.) Su XT, Reyes JV, Lackey AE, Demirci H, Bachmann S, Maeoka Y, Cornelius RJ, McCormick JA, Yang CL, Jung HJ, Welling PA, Nelson JW, Ellison DH (2024 Jan 19). Enriched Single-Nucleus RNA-Sequencing Reveals Unique Attributes of Distal Convoluted Tubule Cells Journal of the American Society of Nephrology : JASN, 35(4), 426-440. . PMCID: 11000721.

Description:

The Integrated Genomics Lab (IGL) provides a range of genomics services. These include nucleic acid isolation, qPCR, and sequencing library preparation for bulk RNA and DNA and for single-cell RNA-seq using the 10x Genomics Chromium iX. Sequencing is done using either a NovaSeq 6000 or a NextSeq 2000. The IGL also offers alignment of bulk RNA sequencing data and single-cell RNA-seq data. In addition, the IGL provides an oligonucleotide ordering service through IDT and a cell line authentication service.