Emory University
Ricardo Guerrero-Ferreira
Emerson Site, Cherry L. Emerson Hall, Suite E106, 1521 Dickey Drive NE
BioConnector Site, Biochemistry Connector, Suite G236, 1510 Clifton Rd.
Atlanta, GA 30322
https://www.cores.emory.edu/iemc/
RRID: RRID:SCR_023537
(Equipment No Longer Active At this Facility)
FEI Talos Arctica Cryo-TEM
Transmission electron microscope that is built for delivering high-resolution 3D characterization of biological samples and biomaterials in cell biology, structural biology, and nanotechnology research. [Product Link]
RRID:SCR_019905
(Equipment No Longer Active At this Facility)
ThermoFisher Talos Arctica Cryo-Transmission Electron Microscope
Autoloader system for cryo-EM single particle and tomography data acquisition
ThermoFisher Talos L120C TEM and STEM
Transmission electron microscope for conventional and cryo-EM
Cell Imaging
Computational - High Performance Computing
Correlative Light Electron Microscopy
Cryo Light Microscopy
Cryo-Electron Microscopy
Cryo-Electron Microscopy Of Vitrified Sections (CEMOVIS)
Cryo-Electron Tomography
Cryo-Scanning Electron Microscopy
Cryo-Ultra Microtomy
Electron Microscopy
Freeze Substitution
High-Pressure Freezing
Histology
Immuno Electron Microscopy
Immunofluorescence
Immunohistochemistry
Microscopy
Microstructure Characterization
Microtomy/Electron
Microtomy/Light
Natural Product Analysis
Negative Staining For TEM
Scanning Electron Microscopy
Sputter Coating
Tissue Embed
Transmission Electron Microscopy
1.) Yang Z, Ghorai N, Wu S, He S, Lian T (2025 Jan 14). Direct and Indirect Interfacial Electron Transfer at a Plasmonic p-Cu(7)S(4)/CdS Heterojunction. ACS nano, 19(1), 1547-1556. . PMID: 39743773.
2.) Mazeaud C, Pfister S, Owen JE, Pereira HS, Charbonneau F, Robinson ZE, Anton A, Bemis CL, Sow AA, Patel TR, Neufeldt CJ, Scaturro P, Chatel-Chaix L (2024 Nov 20). Zika virus remodels and hijacks IGF2BP2 ribonucleoprotein complex to promote viral replication organelle biogenesis. eLife, 13(), . . PMID: 39565347.
3.) Kshirsagar SM, Shrestha N, Kipping T, Banga AK (2024 Jul). Formulation development of tazarotene-loaded PLGA nanoparticles for follicular delivery in the treatment of inflammatory skin diseases. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 200(), 114346. doi: 10.1016/j.ejpb.2024.114346. PMID: 38823541.
4.) Kshirsagar SM, Viswaroopan N, Ghosh M, Junaid MSA, Haque S, Khan J, Muzaffar S, Srivastava RK, Athar M, Banga AK (2024 May 27). Development of 4-phenylbutyric acid microsponge gel formulations for the treatment of lewisite-mediated skin injury. Drug delivery and translational research, (), . . PMID: 38802678.
5.) Knippler CM, Arnst JL, Robinson IE, Matsuk V, Khatib TO, Harvey RD, Shanmugam M, Mouw JK, Fu H, Ganesh T, Marcus AI (2024 Mar 27). Bisbiguanide analogs induce mitochondrial stress to inhibit lung cancer cell invasion iScience, 27(4), 109591. . PMCID: 11022046.
6.) McFadden WM, Casey-Moore MC, Bare GAL, Kirby KA, Wen X, Li G, Wang H, Slack RL, Snyder AA, Lorson ZC, Kaufman IL, Cilento ME, Tedbury PR, Gembicky M, Olson AJ, Torbett BE, Sharpless KB, Sarafianos SG (2024 Mar 21). Identification of clickable HIV-1 capsid-targeting probes for viral replication inhibition. Cell chemical biology, 31(3), 477-486.e7. . PMID: 38518746.
7.) Radmard A, Banga AK (2024 Feb 22). Microneedle-Assisted Transdermal Delivery of Lurasidone Nanoparticles. Pharmaceutics, 16(3), . . PMID: 38543202.
8.) Arnst J, Jing Z, Cohen C, Ha SW, Viggeswarapu M, Beck GR Jr (2023 Oct). Bioactive silica nanoparticles target autophagy, NF-κB, and MAPK pathways to inhibit osteoclastogenesis. Biomaterials, 301(), 122238. doi: 10.1016/j.biomaterials.2023.122238. ID: 37441901.
9.) Kelvin JM, Jain J, Thapa A, Qui M, Birnbaum LA, Moore SG, Zecca H, Summers RJ, Switchenko JM, Costanza E, Uricoli B, Wang X, Jui NT, Fu H, Du Y, DeRyckere D, Graham DK, Dreaden EC (2023 Sep 13). Constitutively Synergistic Multiagent Drug Formulations Targeting MERTK, FLT3, and BCL-2 for Treatment of AML. Pharmaceutical research, (), . doi: 10.1007/s11095-023-03596-9. ID: 37704893.
10.) Patel A, Kumar S, Lai L, Keen M, Valanparambil R, Chakravarthy C, Laughlin Z, Frank F, Cheedarla N, Verkerke HP, Neish AS, Roback JD, Davis CW, Wrammert J, Sharma A, Ahmed R, Suthar MS, Murali-Krishna K, Chandele A, Ortlund E (2023 Sep 13). Light chain of a public SARS-CoV-2 class-3 antibody modulates neutralization against Omicron. Cell reports, 42(9), 113150. doi: 10.1016/j.celrep.2023.113150. ID: 37708028.
11.) Ramey-Ward AN, Dong Y, Yang J, Ogasawara H, Bremer-Sai EC, Brazhkina O, Franck C, Davis M, Salaita K (2023 Sep 11). Optomechanically Actuated Hydrogel Platform for Cell Stimulation with Spatial and Temporal Resolution. ACS biomaterials science & engineering, 9(9), 5361-5375. . ID: 37604774.
12.) Kelvin JM, Chimenti ML, Zhang DY, Williams EK, Moore SG, Humber GM, Baxter TA, Birnbaum LA, Qui M, Zecca H, Thapa A, Jain J, Jui NT, Wang X, Fu H, Du Y, Kemp ML, Lam WA, Graham DK, DeRyckere D, Dreaden EC (2023 Sep). Development of constitutively synergistic nanoformulations to enhance chemosensitivity in T-cell leukemia. Journal of controlled release : official journal of the Controlled Release Society, 361(), 470-482. doi: 10.1016/j.jconrel.2023.07.045. ID: 37543290.
13.) Zhao AX, Zhu YI, Chung E, Lee J, Morais S, Yoon H, Emelianov S (2023 Aug 2). Factors Influencing the Repeated Transient Optical Droplet Vaporization Threshold and Lifetimes of Phase Change, Perfluorocarbon Nanodroplets. Nanomaterials (Basel, Switzerland), 13(15), . . ID: 37570555.
14.) He X, Jarrell ZR, Smith MR, Ly VT, Hu X, Sueblinvong V, Liang Y, Orr M, Go YM, Jones DP (2023 Aug 1). Low-dose vanadium pentoxide perturbed lung metabolism associated with inflammation and fibrosis signaling in male animal and in vitro models. American journal of physiology. Lung cellular and molecular physiology, 325(2), L215-L232. . ID: 37310758.
15.) Santiago JV, Natu A, Ramelow CC, Rayaprolu S, Xiao H, Kumar V, Seyfried NT, Rangaraju S (2023 Jul 29). Identification of state-specific proteomic and transcriptomic signatures of microglia-derived extracellular vesicles. bioRxiv : the preprint server for biology, (), . . ID: 37546899.
16.) Beckwith SL, Nomberg EJ, Newman AC, Taylor JV, Guerrero-Ferreira RC, Garfinkel DJ (2023 Jul 25). An interchangeable prion-like domain is required for Ty1 retrotransposition. Proceedings of the National Academy of Sciences of the United States of America, 120(30), e2303358120. . ID: 37459521.
17.) Patel A, Kumar S, Lai L, Chakravarthy C, Valanparambil R, Reddy ES, Gottimukkala K, Bajpai P, Raju DR, Edara VV, Davis-Gardner ME, Linderman S, Dixit K, Sharma P, Mantus G, Cheedarla N, Verkerke HP, Frank F, Neish AS, Roback JD, Davis CW, Wrammert J, Ahmed R, Suthar MS, Sharma A, Murali-Krishna K, Chandele A, Ortlund EA (2023 Jul 6). Molecular basis of SARS-CoV-2 Omicron variant evasion from shared neutralizing antibody response. Structure (London, England : 1993), 31(7), 801-811.e5. . ID: 37167972.
18.) Srinivas P, Nosrati M, Zelinskaya N, Dey D, Comstock LR, Dunham CM, Conn GL (2023 Jun 20). 30S subunit recognition and G1405 modification by the aminoglycoside-resistance 16S ribosomal RNA methyltransferase RmtC. Proceedings of the National Academy of Sciences of the United States of America, 120(25), e2304128120. . ID: 37307464.
19.) Ohanele C, Peoples JN, Karlstaedt A, Geiger JT, Gayle AD, Ghazal N, Sohani F, Brown ME, Davis ME, Porter GA, Faundez V, Kwong JQ (2023 May 22). Mitochondrial citrate carrier SLC25A1 is a dosage-dependent regulator of metabolic reprogramming and morphogenesis in the developing heart. bioRxiv : the preprint server for biology, (), . . ID: 37292906.
20.) Salazar-Noratto GE, Nations CC, Stevens HY, Xu M, Gaynard S, Dooley C, de Nijs N, McDonagh K, Shen S, Willimon SC, Barry F, Guldberg RE (2023 May 15). Patient-Specific iPSC-Derived Models Link Aberrant Endoplasmic Reticulum Stress Sensing and Response to Juvenile Osteochondritis Dissecans Etiology. Stem cells translational medicine, 12(5), 293-306. . ID: 37184892.
21.) Zhang L, Hajebrahimi S, Tong S, Gao X, Cheng H, Zhang Q, Hinojosa DT, Jiang K, Hong L, Huard J, Bao G (2023 May 5). Force-Mediated Endocytosis of Iron Oxide Nanoparticles for Magnetic Targeting of Stem Cells. ACS applied materials & interfaces, (), . doi: 10.1021/acsami.2c20265. ID: 37145890.
22.) Hellen DJ, Bennett A, Malla S, Klindt C, Rao A, Dawson PA, Karpen SJ (2023 Apr 1). Liver-restricted deletion of the biliary atresia candidate gene Pkd1l1 causes bile duct dysmorphogenesis and ciliopathy. Hepatology (Baltimore, Md.), 77(4), 1274-1286. doi: 10.1097/HEP.0000000000000029. ID: 36645229.
23.) Park HJ, Hoffman JR, Brown ME, Bheri S, Brazhkina O, Son YH, Davis ME (2023 Mar 3). Knockdown of deleterious miRNA in progenitor cell-derived small extracellular vesicles enhances tissue repair in myocardial infarction. Science advances, 9(9), eabo4616. . ID: 36867699.
24.) Rotolo L, Vanover D, Bruno NC, Peck HE, Zurla C, Murray J, Noel RK, O (2023 Mar). Species-agnostic polymeric formulations for inhalable messenger RNA delivery to the lung. Nature materials, 22(3), 369-379. doi: 10.1038/s41563-022-01404-0. ID: 36443576.
25.) Gao Y, Raghavan A, Deng B, Lee J, Liang B (2023 Jan 25). Optimal Conditions for In Vitro Assembly of Respiratory Syncytial Virus Nucleocapsid-like Particles. Viruses, 15(2), . . ID: 36851557.
26.) Xiao X, Robang AS, Sarma S, Le JV, Helmicki ME, Lambert MJ, Guerrero-Ferreira R, Arboleda-Echavarria J, Paravastu AK, Hall CK (2022 Nov). Sequence patterns and signatures: Computational and experimental discovery of amyloid-forming peptides. PNAS nexus, 1(5), pgac263. . ID: 36712347.
27.) Kreutzberger MAB, Wang S, Beltran LC, Tuachi A, Zuo X, Egelman EH, Conticello VP (2022 May 17). Phenol-soluble modulins PSMα3 and PSMβ2 form nanotubes that are cross-α amyloids. Proceedings of the National Academy of Sciences of the United States of America, 119(20), e2121586119. . ID: 35533283.
28.) Srinivas P, Steiner RE, Pavelich IJ, Guerrero-Ferreira R, Juneja P, Ibba M, Dunham CM (2021 Nov 18). Oxidation alters the architecture of the phenylalanyl-tRNA synthetase editing domain to confer hyperaccuracy. Nucleic acids research, 49(20), 11800-11809. . ID: 34581811.
29.) Wang F, Gnewou O, Wang S, Osinski T, Zuo X, Egelman EH, Conticello VP (2021 Oct 6). Deterministic chaos in the self-assembly of β sheet nanotubes from an amphipathic oligopeptide. Matter, 4(10), 3217-3231. . ID: 34632372.
30.) Martin MD, Huard DJE, Guerrero-Ferreira RC, Desai IM, Barlow BM, Lieberman RL (2021 Oct). Molecular architecture and modifications of full-length myocilin. Experimental eye research, 211(), 108729. . ID: 34400147.
31.) Cho J, Kim S, Lee H, Rah W, Cho HC, Kim NK, Bae S, Shin DH, Lee MG, Park IH, Tanaka Y, Shin E, Yi H, Han JW, Hwang PTJ, Jun HW, Park HJ, Cho K, Lee SW, Jung JK, Levit RD, Sussman MA, Harvey RP, Yoon YS (2021 Aug). Regeneration of infarcted mouse hearts by cardiovascular tissue formed via the direct reprogramming of mouse fibroblasts. Nature biomedical engineering, 5(8), 880-896. . ID: 34426676.
32.) Wang F, Gnewou O, Modlin C, Beltran LC, Xu C, Su Z, Juneja P, Grigoryan G, Egelman EH, Conticello VP (2021 Jan 18). Structural analysis of cross α-helical nanotubes provides insight into the designability of filamentous peptide nanomaterials. Nature communications, 12(1), 407. . ID: 33462223.
33.) Merg AD, Touponse G, Genderen EV, Blum TB, Zuo X, Bazrafshan A, Siaw HMH, McCanna A, Brian Dyer R, Salaita K, Abrahams JP, Conticello VP (2020 Nov 25). Shape-Shifting Peptide Nanomaterials: Surface Asymmetry Enables pH-Dependent Formation and Interconversion of Collagen Tubes and Sheets. Journal of the American Chemical Society, 142(47), 19956-19968. doi: 10.1021/jacs.0c08174. ID: 33170675.
34.) Tillman MC, Imai N, Li Y, Khadka M, Okafor CD, Juneja P, Adhiyaman A, Hagen SJ, Cohen DE, Ortlund EA (2020 Sep 8). Allosteric regulation of thioesterase superfamily member 1 by lipid sensor domain binding fatty acids and lysophosphatidylcholine. Proceedings of the National Academy of Sciences of the United States of America, 117(36), 22080-22089. . ID: 32820071.
35.) Cao D, Gao Y, Roesler C, Rice S, D (2020 Jan 17). Cryo-EM structure of the respiratory syncytial virus RNA polymerase. Nature communications, 11(1), 368. . ID: 31953395.
36.) Xu Y, Wu H, Xiong Q, Ji B, Yi H, Duan H, Mao H (2019 Aug 19). Size-Controllable Magnetic Iron Oxide Nanorods for Biomarker Targeting and Improving Microfluidic Mixing. ACS applied bio materials, 2(8), 3362-3371. doi: 10.1021/acsabm.9b00359. ID: 35030778.
37.) Ke Z, Dillard RS, Chirkova T, Leon F, Stobart CC, Hampton CM, Strauss JD, Rajan D, Rostad CA, Taylor JV, Yi H, Shah R, Jin M, Hartert TV, Peebles RS Jr, Graham BS, Moore ML, Anderson LJ, Wright ER (2018 Aug 20). The Morphology and Assembly of Respiratory Syncytial Virus Revealed by Cryo-Electron Tomography. Viruses, 10(8), . . ID: 30127286.
38.) Abreu-Velez AM, Howard MS, Yi H, Florez-Vargas AA (2018 Aug). Patients affected by a new variant of endemic pemphigus foliaceus have autoantibodies colocalizing with MYZAP, p0071, desmoplakins 1-2 and ARVCF, causing renal damage. Clinical and experimental dermatology, 43(6), 692-702. doi: 10.1111/ced.13566. ID: 29768670.
39.) Ke Z, Strauss JD, Hampton CM, Brindley MA, Dillard RS, Leon F, Lamb KM, Plemper RK, Wright ER (2018 Apr 30). Promotion of virus assembly and organization by the measles virus matrix protein. Nature communications, 9(1), 1736. . ID: 29712906.
40.) Abreu-Velez AM, Yi H, Howard MS (2017 Oct). Cell junction protein armadillo repeat gene deleted in velo-cardio-facial syndrome is expressed in the skin and colocalizes with autoantibodies of patients affected by a new variant of endemic pemphigus foliaceus in Colombia. Dermatology practical & conceptual, 7(4), 3-8. . ID: 29214101.
The Robert P. Apkarian Integrated Electron Microscopy Core, one of the Emory Integrated Core Facilities (EICF), is supported by the National Institutes of Health, the National Science Foundation, the Georgia Clinical and Translational Science Alliance and the Emory University School of Medicine. We help investigators use the latest technologies on structural research in their projects. Please contact us so that we can discuss your experimental needs.
