Hitachi HT7700 120 kV TEM

The Hitachi HT7700 TEM has a Tungsten filament and it is used for imaging biological and materials samples. It is equipped with an AMT CCD camera. This instrument is capable of tilt imaging with a motorized goniometer which allows +/- 70° sample tilting.

Citation IDs: Georgia Clinical and Translational Science Alliance under award number UL1TR002378

cite this instrument

JEOL JEM-1400 Transmission Electron Microscope

The JEOL JEM-1400 120 kV LaB6TEM is equipped with a Gatan, 2k x 2k, CCD camera. There are two Gatan 626 cryo holders available for acquisition of cryo-TEM data. The JEOL JEM-1400 is capable of several modes of TEM, including tomography of sectioned materials, cryo-TEM, and room temperature TEM. Semi-automated data acquisition using Serial EM is available for testing cryo-TEM grids, and a Minimum Dose System (MDS) funtion allows for imaging of beam-sensitive samples. The beam blocker allows for electron diffraction experiments.

Citation IDs: National Institutes of Health Grant S10 RR025679

cite this instrument

JEOL JEM-2200FS 200 kV TEM

The JEOL JEM-2200FS 200kV Field Emission TEM has two direct electron detectors, a Direct Electron DE-20 and a Gatan K2. Images can be acquired with improved contrast by the use of Zernike or hole-free phase plates. An in-column energy filter (Omega filter) is specially important for high-resolution cryo-electron tomography and cryo-electron microscopy of thick samples. SerialEM software is available for semi-automated acquisition of cryo-EM, cryo-ET, and microcrystal electron difraction data. Two Gatan 914 cryo holders are available for tilt series acquisition and imaging of cryo-TEM specimens.

Citation IDs: National Science Foundation Major Research Instrumentation Grant 0923395

cite this instrument

JEOL JSM-IT700HR SEM

The JEOL JSM-IT700HR is an analytical, high-resolution, field emission scanning electron microscope (SEM) capable of imaging under high and low vacuum conditions. It is used for imaging biological samples and materials. A specialized MP-94370LSED Low Vacuum Secondary Electron Detector allows acquisition of structural data for soft, uncoated, hydrated samples. It is equipped with both a secondary electron detector and a high-sensitivity, multi-segment, solid-state backscattered electron detector. This instrument features a large analytical chamber and a fully embedded JEOL JED-2300 energy-dispersive micro-analysis system (EDS) with a silicon drift detector (SDD).

Leica DM6 FS cryo-CLEM fluorescence microscope

The Leica Cryo-CLEM system for Correlated Light and Electron Microscopy (CLEM) is a cryo fluorescence microscope which allows the observation of frozen-hydrated samples from viruses to cells on cryo-electron microscopy (cryo-TEM) grids. Data acquisition on this instrument permits mapping of cryo-EM grids and transferring of coordinates to a transmission electron microscope for further, high-resolution, data collection with an automated system such as Serial EM.

ThermoFisher Hydra Bio Plasma FIB-SEM

Thermo Scientific Hydra Bio Plasma Focused Ion Beam-Scanning Electron Microscope with a Delmic METEOR integrated Fluorescent Light Microscope (iFLM) for Correlated Light and Electron Microscopy. This instrument is equipped with 4 ion sources (Xenon, Argon, Oxygen, and Nitrogen) to allow for multiple FIB-SEM applications on biological samples and materials. Spin-Mill capabilities increases throughput in room temperature volume EM by acquiring data in multiple regions of interest within a single sample. Cryo applications convert this instrument in a sample prepartion tool for lamella milling, as well as a data acquisition tool for cryo volume EM.

Citation IDs: National Institutes of Health Grant S10 1S10OD034303-01

cite this instrument

ThermoFisher Talos Arctica 200 kv TEM

The ThermoFisher Talos Arctica Field Emission TEM has a BioQuantum Gatan K3 direct electron detector. Images are acquired using a Gatan Image Filter system (GIF) which is especially useful for high-resolution cryo-electron tomography, cryo-electron microscopy of thick samples, and to improve contrast on cryo-electron microscopy specimens. This instrument is equipped with an autoloader system, capable of loading 12 samples at a time, which can be maintained under liquid nitrogen temperatures for easy sample exchanges. Single particle data and tilt series acquisition are done using Serial EM. The ThermoFisher EPU software can also be used for the automated acquisition of single-particle cryo-EM data.

ThermoFisher Talos L120C TEM and STEM

Transmission electron microscope for conventional and cryo-EM