MRC Protein Phosphorylation and Ubiquitylation Unit
Francesca Tonelli
MRC PPU Reagents and Services, School of Life Sciences, University of Dundee
Dundee DD1 5EH
https://mrcppureagents.dundee.ac.uk/
RRID: RRID:SCR_023283
Access To Clones
Antibody Characterization
Antibody Development
Antibody Production
Assay Development
Assays and Measurements
Automated Liquid Handling
Biochemical Analysis
CDNA Synthesis
Cloning
CRISPR
CRISPR/Cas Project Design
Dub Profiling
Inhibitor Screening
Kinase Screening
Protein Extraction/Purification
Reagents
Repository
Sequencing - DNA Sequencing
1.) Waschbüsch D, Pal P, Nirujogi RS, Cavin M, Singh J, Alessi DR, Khan AR (2025 Aug 24). Structural basis for binding of RILPL1 to TMEM55B reveals a lysosomal platform for adaptor assembly through a conserved TBM motif. bioRxiv : the preprint server for biology, (), . . PMID: 40894729.
2.) Amnekar RV, Dite T, Lis P, Bell S, Brown F, Johnson C, Wilkinson S, Raggett S, Dorward M, Wightman M, Macartney T, Soares RF, Lamoliatte F, Alessi DR (2025 Jul 18). NRBP1 pseudokinase binds to and activates the WNK pathway in response to osmotic stress. Science advances, 11(29), eadv4636. . PMID: 40668933.
3.) Glennie L, Curnutt N, Cartwright T, Dunbar K, Le Chatelier B, Wood NT, Macartney TJ, Woo CM, Sapkota GP (2025 Jun 17). The contribution of native protein complexes to targeted protein degradation. bioRxiv : the preprint server for biology, (), . . PMID: 40667143.
4.) Singh PK, Agarwal S, Volpi I, Wilhelm LP, Becchi G, Keenlyside A, Macartney T, Toth R, Rousseau A, Masson GR, Ganley IG, Muqit MMK (2025 May 9). Kinome screening identifies integrated stress response kinase EIF2AK1/HRI as a negative regulator of PINK1 mitophagy signaling. Science advances, 11(19), eadn2528. . PMID: 40344059.
5.) Chen Z, Chi G, Balo T, Chen X, Montes BR, Clifford SC, D'Angiolella V, Szabo T, Kiss A, Novak T, Herner A, Kotschy A, Bullock AN (2025 Apr 2). Structural mimicry of UM171 and neomorphic cancer mutants co-opts E3 ligase KBTBD4 for HDAC1/2 recruitment. Nature communications, 16(1), 3144. . PMID: 40175372.
6.) Lim SY, Toh TS, Hor JW, Lim JL, Lit LC, Ahmad-Annuar A, Tay YW, Foo JN, Ng EY, Muthusamy KA, Mohamed Ibrahim N, Ibrahim KA, Tan LCS, Zulkefli J, Khairul Anuar AN, Black K, Lis P, Xie F, Cen Z, Lim KS, Lohmann K, Padmanabhan S, Alessi DR, Luo W, Tan EK, Sammler E, Tan AH (2025 Feb 23). Clinical and functional evidence for the pathogenicity of the LRRK2 p.Arg1067Gln variant. NPJ Parkinson's disease, 11(1), 34. . PMID: 39988587.
7.) Madsen RR, Le Marois A, Mruk ON, Voliotis M, Yin S, Sufi J, Qin X, Zhao SJ, Gorczynska J, Morelli D, Davidson L, Sahai E, Korolchuk VI, Tape CJ, Vanhaesebroeck B (2025 Feb). Oncogenic PIK3CA corrupts growth factor signaling specificity. Molecular systems biology, 21(2), 126-157. . PMID: 39706867.
8.) Bandi V, Rennie M, Koch I, Gill P, Pacheco OD, Berg AD, Cui H, Ward DI, Bustos F (2025 Jan 9). RLIM-specific activity reporters define variant pathogenicity in Tonne-Kalscheuer syndrome. HGG advances, 6(1), 100378. . PMID: 39482882.
9.) Saarela D, Lis P, Gomes S, Nirujogi RS, Dong W, Rawat E, Glendinning S, Zeneviciute K, Bagnoli E, Fasimoye R, Lin C, Nyame K, Boros FA, Zunke F, Lamoliatte F, Elshani S, Jaconelli M, Jans JJ, Huisman MA, Posern C, Westermann LM, Schulz A, van Hasselt PM, Alessi DR, Abu-Remaileh M, Sammler EM (2024 Dec 26). Tagless LysoIP for immunoaffinity enrichment of native lysosomes from clinical samples. The Journal of clinical investigation, 135(4), . . PMID: 39724071.
10.) Lange SM, McFarland MR, Lamoliatte F, Carroll T, Krshnan L, Pérez-Ràfols A, Kwasna D, Shen L, Wallace I, Cole I, Armstrong LA, Knebel A, Johnson C, De Cesare V, Kulathu Y (2024 Dec). VCP/p97-associated proteins are binders and debranching enzymes of K48-K63-branched ubiquitin chains. Nature structural & molecular biology, 31(12), 1872-1887. . PMID: 38977901.
11.) Snelling T (2024 Nov 20). Quantitative Measurement of the Kinase Activity of Wildtype ALPK1 and Disease-Causing ALPK1 Mutants Using Cell-Free Radiometric Phosphorylation Assays. Bio-protocol, 14(22), e5124. . PMID: 39600973.
12.) Snelling T (2024 Nov 20). Measurement of the Activity of Wildtype and Disease-Causing ALPK1 Mutants in Transfected Cells With a 96-Well Format NF-κB/AP-1 Reporter Assay. Bio-protocol, 14(22), e5113. . PMID: 39600975.
13.) Tejwani V, Carroll T, Macartney T, Bandau S, Alabert C, Saredi G, Toth R, Rouse J (2024 Sep 6). PROTAC-mediated conditional degradation of the WRN helicase as a potential strategy for selective killing of cancer cells with microsatellite instability. Scientific reports, 14(1), 20824. . PMID: 39242638.
14.) Polo Rivera C, Deegan TD, Labib KPM (2024 Sep). CMG helicase disassembly is essential and driven by two pathways in budding yeast. The EMBO journal, 43(18), 3818-3845. . PMID: 39039287.
15.) Brewer A, Zhao JF, Fasimoye R, Shpiro N, Macartney TJ, Wood NT, Wightman M, Alessi DR, Sapkota GP (2024 Aug 16). Targeted dephosphorylation of SMAD3 as an approach to impede TGF-β signaling. iScience, 27(8), 110423. . PMID: 39104417.
16.) Szulc NA, Stefaniak F, Piechota M, Soszyńska A, Piórkowska G, Cappannini A, Bujnicki JM, Maniaci C, Pokrzywa W (2024 Jul 5). DEGRONOPEDIA: a web server for proteome-wide inspection of degrons. Nucleic acids research, 52(), W221-W232. . PMID: 38567734.
17.) Raimi OG, Ojha H, Ehses K, Dederer V, Lange SM, Rivera CP, Deegan TD, Chen Y, Wightman M, Toth R, Labib KPM, Mathea S, Ranson N, Fernández-Busnadiego R, Muqit MMK (2024 Jun 7). Mechanism of human PINK1 activation at the TOM complex in a reconstituted system. Science advances, 10(23), eadn7191. . PMID: 38848361.
18.) Abdul Rehman SA, Cazzaniga C, Di Nisio E, Antico O, Knebel A, Johnson C, Şahin AT, Ibrahim PEGF, Lamoliatte F, Negri R, Muqit MMK, De Cesare V (2024 Mar 29). Discovery and characterization of noncanonical E2-conjugating enzymes. Science advances, 10(13), eadh0123. . PMID: 38536929.
19.) Sanchez-Lopez I, Orantos-Aguilera Y, Pozo-Guisado E, Alvarez-Barrientos A, Lilla S, Zanivan S, Lachaud C, Martin-Romero FJ (2024 Mar 21). STIM1 translocation to the nucleus protects cells from DNA damage. Nucleic acids research, 52(5), 2389-2415. . PMID: 38224453.
20.) Brewer A, Sathe G, Pflug BE, Clarke RG, Macartney TJ, Sapkota GP (2024 Mar 15). Mapping the substrate landscape of protein phosphatase 2A catalytic subunit PPP2CA. iScience, 27(3), 109302. . PMID: 38450154.
21.) Koch I, Slovik M, Zhang Y, Liu B, Rennie M, Konz E, Cogne B, Daana M, Davids L, Diets IJ, Gold NB, Holtz AM, Isidor B, Mor-Shaked H, Neira Fresneda J, Niederhoffer KY, Nizon M, Pfundt R, Simon M, Stegmann A, Guillen Sacoto MJ, Wevers M, Barakat TS, Yanovsky-Dagan S, Atanassov BS, Toth R, Gao C, Bustos F, Harel T (2024 Mar). USP27X variants underlying X-linked intellectual disability disrupt protein function via distinct mechanisms. Life science alliance, 7(3), . . PMID: 38182161.
22.) Makhlouf L, Peter JJ, Magnussen HM, Thakur R, Millrine D, Minshull TC, Harrison G, Varghese J, Lamoliatte F, Foglizzo M, Macartney T, Calabrese AN, Zeqiraj E, Kulathu Y (2024 Mar). The UFM1 E3 ligase recognizes and releases 60S ribosomes from ER translocons. Nature, 627(8003), 437-444. . PMID: 38383789.
23.) Traynor R, Moran J, Stevens M, Antico O, Knebel A, Behrouz B, Merchant K, Hastie CJ, Davies P, Muqit MMK, De Cesare V (2024 Feb 26). Design and high-throughput implementation of MALDI-TOF/MS-based assays for Parkin E3 ligase activity. Cell reports methods, 4(2), 100712. . PMID: 38382522.
24.) Phung TK, Berndsen K, Shastry R, Phan TLCHB, Muqit MMK, Alessi DR, Nirujogi RS (2024 Feb 13). CURTAIN-A unique web-based tool for exploration and sharing of MS-based proteomics data. Proceedings of the National Academy of Sciences of the United States of America, 121(7), e2312676121. . PMID: 38324566.
25.) Zhu H, Tonelli F, Turk M, Prescott A, Alessi DR, Sun J (2023 Dec 22). Rab29-dependent asymmetrical activation of leucine-rich repeat kinase 2. Science (New York, N.Y.), 382(6677), 1404-1411. . PMID: 38127736.
26.) Pal P, Taylor M, Lam PY, Tonelli F, Hecht CA, Lis P, Nirujogi RS, Phung TK, Yeshaw WM, Jaimon E, Fasimoye R, Dickie EA, Wightman M, Macartney T, Pfeffer SR, Alessi DR (2023 Dec 15). Parkinson's VPS35[D620N] mutation induces LRRK2-mediated lysosomal association of RILPL1 and TMEM55B. Science advances, 9(50), eadj1205. . PMID: 38091401.
27.) Ross FA, Hawley SA, Russell FM, Goodman N, Hardie DG (2023 Dec 13). Frequent loss-of-function mutations in the AMPK-α2 catalytic subunit suggest a tumour suppressor role in human skin cancers. The Biochemical journal, 480(23), 1951-1968. . PMID: 37962491.
28.) Evrin C, Alvarez V, Ainsworth J, Fujisawa R, Alabert C, Labib KP (2023 Nov 6). DONSON is required for CMG helicase assembly in the mammalian cell cycle. EMBO reports, 24(11), e57677. . PMID: 37781960.
29.) Xia Y, Sonneville R, Jenkyn-Bedford M, Ji L, Alabert C, Hong Y, Yeeles JTP, Labib KPM (2023 Sep 22). DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans. Science (New York, N.Y.), 381(6664), eadi4932. . PMID: 37590372.
30.) Gregorczyk M, Pastore G, Muñoz I, Carroll T, Streubel J, Munro M, Lis P, Lange S, Lamoliatte F, Macartney T, Toth R, Brown F, Hastie J, Pereira G, Durocher D, Rouse J (2023 Jul). Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases. Life science alliance, 6(7), . . PMID: 37188479.
31.) Fasimoye R, Dong W, Nirujogi RS, Rawat ES, Iguchi M, Nyame K, Phung TK, Bagnoli E, Prescott AR, Alessi DR, Abu-Remaileh M (2023 May 16). Golgi-IP, a tool for multimodal analysis of Golgi molecular content. Proceedings of the National Academy of Sciences of the United States of America, 120(20), e2219953120. . PMID: 37155866.
32.) Hanna JC, Corpas-Lopez V, Seizova S, Colon BL, Bacchetti R, Hall GMJ, Sands EM, Robinson L, Baragaña B, Wyllie S, Pawlowic MC (2023). Mode of action studies confirm on-target engagement of lysyl-tRNA synthetase inhibitor and lead to new selection marker for Cryptosporidium. Frontiers in cellular and infection microbiology, 13(), 1236814. . PMID: 37600947.
33.) Blanca Ramírez M, Lara Ordóñez AJ, Fdez E, Madero-Pérez J, Gonnelli A, Drouyer M, Chartier-Harlin MC, Taymans JM, Bubacco L, Greggio E, Hilfiker S (2017 Jul 15). GTP binding regulates cellular localization of Parkinson's disease-associated LRRK2. Human molecular genetics, 26(14), 2747-2767. . PMID: 28453723.
As one of the world\'s leading centres focused on cell signalling pathways, we provide access to the very same reagents and services used by MRC PPU investigators to accelerate research worldwide.
