University of Rochester Medical Center
Karen Bentley
601 Elmwood Avenue
Box 626
Rochester, NY 14642
https://www.urmc.rochester.edu/research/electron-microscope.aspx
RRID: RRID:SCR_012366
FEI Talos L120C TEM and STEM
Transmission electron microscope that is designed for performance and productivity across a wide range of samples and applications, such as 2D and 3D imaging of cells, cell organelles, asbestos, polymers, and soft materials, both at ambient and cryogenic temperatures. [Product Link]
RRID:SCR_019908
Hitachi Transmission Electron Microscope
H-7650
Leica Ultramicrotome
UC-7
Tousimis Critical Point Dryer
931 Series
Zeiss Scanning Electron Microscope
Auriga
Biological photography/Photomicrography
Cell Imaging
Consultation
Consultations
Cryo-Electron Microscopy
Electron Microscopy
Image Aquisition
Immuno Electron Microscopy
Microscopy
Microtomy/Electron
Sample Preparation
Scanning Electron Microscopy
Transmission Electron Microscopy
1.) Beutner G, Burris JR, Collins MP, Kulkarni CA, Nadtochiy SM, de Mesy Bentley KL, Cohen ED, Brookes PS, Porter GA Jr (2024 Feb 9). Coordinated metabolic responses to cyclophilin D deletion in the developing heart iScience, 27(3), 109157. . PMCID: 10897919.
2.) Shah T, Leurgans SE, Mehta RI, Yang J, Galloway CA, de Mesy Bentley KL, Schneider JA, Mehta RI (2023 Feb 6). Arachnoid granulations are lymphatic conduits that communicate with bone marrow and dura-arachnoid stroma. The Journal of experimental medicine, 220(2), . . ID: 36469302.
3.) Mestre H, Verma N, Greene TD, Lin LA, Ladron-de-Guevara A, Sweeney AM, Liu G, Thomas VK, Galloway CA, de Mesy Bentley KL, Nedergaard M, Mehta RI (2022 Jul 6). Periarteriolar spaces modulate cerebrospinal fluid transport into brain and demonstrate altered morphology in aging and Alzheimer Nature communications, 13(1), 3897. . ID: 35794106.
4.) Ren Y, Xue T, Rainbolt J, Bentley KLM, Galloway CA, Liu Y, Cherian P, Neighbors J, Hofstee MI, Ebetino FH, Moriarty TF, Sun S, Schwarz EM, Xie C (2022). Efficacy of Bisphosphonate-Conjugated Sitafloxacin in a Murine Model of S. aureus Osteomyelitis: Evidence of "Target & Release" Kinetics and Killing of Bacteria Within Canaliculi. Frontiers in cellular and infection microbiology, 12(), 910970. . ID: 35811672.
5.) Adjei-Sowah E, Peng Y, Weeks J, Jonason JH, de Mesy Bentley KL, Masters E, Morita Y, Muthukrishnan G, Cherian P, Hu XE, McKenna CE, Ebetino FH, Sun S, Schwarz EM, Xie C (2021 Jun 17). Development of Bisphosphonate-Conjugated Antibiotics to Overcome Pharmacodynamic Limitations of Local Therapy: Initial Results with Carbamate Linked Sitafloxacin and Tedizolid. Antibiotics (Basel, Switzerland), 10(6), . . ID: 34204351.
6.) Manian KV, Galloway CA, Dalvi S, Emanuel AA, Mereness JA, Black W, Winschel L, Soto C, Li Y, Song Y, DeMaria W, Kumar A, Slukvin I, Schwartz MP, Murphy WL, Anand-Apte B, Chung M, Benoit DSW, Singh R (2021 May 6). 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration. Cell stem cell, 28(5), 978. . ID: 33961770.
7.) Tang C, Han J, Dalvi S, Manian K, Winschel L, Volland S, Soto CA, Galloway CA, Spencer W, Roll M, Milliner C, Bonilha VL, Johnson TB, Latchney L, Weimer JM, Augustine EF, Mink JW, Gullapalli VK, Chung M, Williams DS, Singh R (2021 Feb 5). A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface. Communications biology, 4(1), 161. . ID: 33547385.
8.) Masters EA, Hao SP, Kenney HM, Morita Y, Galloway CA, de Mesy Bentley KL, Ricciardi BF, Boyce BF, Schwarz EM, Oh I (2021 Feb). Distinct vasculotropic versus osteotropic features of S. agalactiae versus S. aureus implant-associated bone infection in mice. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 39(2), 389-401. . ID: 33336806.
9.) de Mesy Bentley KL, Galloway CA, Muthukrishnan G, Echternacht SR, Masters EA, Zeiter S, Schwarz EM, Leckenby JI (2021 Feb). Emerging electron microscopy and 3D methodologies to interrogate Staphylococcus aureus osteomyelitis in murine models. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 39(2), 376-388. . ID: 33377538.
10.) Masters EA, Muthukrishnan G, Ho L, Gill AL, de Mesy Bentley KL, Galloway CA, McGrath JL, Awad HA, Gill SR, Schwarz EM (2021). Staphylococcus aureus Cell Wall Biosynthesis Modulates Bone Invasion and Osteomyelitis Pathogenesis. Frontiers in microbiology, 12(), 723498. . ID: 34484165.
11.) Nishitani K, Ishikawa M, Morita Y, Yokogawa N, Xie C, de Mesy Bentley KL, Ito H, Kates SL, Daiss JL, Schwarz EM (2020 Oct 2). IsdB antibody-mediated sepsis following S. aureus surgical site infection. JCI insight, 5(19), . . ID: 33004694.
12.) Masters EA, de Mesy Bentley KL, Gill AL, Hao SP, Galloway CA, Salminen AT, Guy DR, McGrath JL, Awad HA, Gill SR, Schwarz EM (2020 Oct). Identification of Penicillin Binding Protein 4 (PBP4) as a critical factor for Staphylococcus aureus bone invasion during osteomyelitis in mice. PLoS pathogens, 16(10), e1008988. . ID: 33091079.
13.) Trombetta RP, de Mesy Bentley KL, Schwarz EM, Kate SL, Awad HA (2019 Jun 27). A murine femoral ostectomy model with hardware exchange to assess antibiotic-impregnated spacers for implant-associated osteomyelitis. European cells & materials, 37(), 431-443. doi: 10.22203/eCM.v037a26. ID: 31243755.
14.) Chen W, Anokhina V, Dieudonne G, Abramowitz MK, Kashyap R, Yan C, Wu TT, de Mesy Bentley KL, Miller BL, Bushinsky DA (2019 Jun 1). Patients with advanced chronic kidney disease and vascular calcification have a large hydrodynamic radius of secondary calciprotein particles. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 34(6), 992-1000. . ID: 29788425.
15.) Trombetta RP, Ninomiya MJ, El-Atawneh IM, Knapp EK, de Mesy Bentley KL, Dunman PM, Schwarz EM, Kates SL, Awad HA (2019 Feb 22). Calcium Phosphate Spacers for the Local Delivery of Sitafloxacin and Rifampin to Treat Orthopedic Infections: Efficacy and Proof of Concept in a Mouse Model of Single-Stage Revision of Device-Associated Osteomyelitis. Pharmaceutics, 11(2), . . ID: 30813284.
16.) Masters EA, Trombetta RP, de Mesy Bentley KL, Boyce BF, Gill AL, Gill SR, Nishitani K, Ishikawa M, Morita Y, Ito H, Bello-Irizarry SN, Ninomiya M, Brodell JD Jr, Lee CC, Hao SP, Oh I, Xie C, Awad HA, Daiss JL, Owen JR, Kates SL, Schwarz EM, Muthukrishnan G (2019). Evolving concepts in bone infection: redefining "biofilm", "acute vs. chronic osteomyelitis", "the immune proteome" and "local antibiotic therapy". Bone research, 7(), 20. . ID: 31646012.
17.) Carli AV, Bhimani S, Yang X, de Mesy Bentley KL, Ross FP, Bostrom MPG (2018 Jun 6). Vancomycin-Loaded Polymethylmethacrylate Spacers Fail to Eradicate Periprosthetic Joint Infection in a Clinically Representative Mouse Model. The Journal of bone and joint surgery. American volume, 100(11), e76. doi: 10.2106/JBJS.17.01100. ID: 29870449.
18.) Ishikawa M, de Mesy Bentley KL, McEntire BJ, Bal BS, Schwarz EM, Xie C (2017 Dec). Surface topography of silicon nitride affects antimicrobial and osseointegrative properties of tibial implants in a murine model. Journal of biomedical materials research. Part A, 105(12), 3413-3421. . ID: 28865177.
19.) Galloway CA, Dalvi S, Hung SSC, MacDonald LA, Latchney LR, Wong RCB, Guymer RH, Mackey DA, Williams DS, Chung MM, Gamm DM, Pébay A, Hewitt AW, Singh R (2017 Sep 26). Drusen in patient-derived hiPSC-RPE models of macular dystrophies. Proceedings of the National Academy of Sciences of the United States of America, 114(39), E8214-E8223. . ID: 28878022.
20.) Barnett RC, Lin X, Barravecchia M, Norman RA, de Mesy Bentley KL, Fazal F, Young JL, Dean DA (2017 Jul). Featured Article: Electroporation-mediated gene delivery of surfactant protein B (SP-B) restores expression and improves survival in mouse model of SP-B deficiency. Experimental biology and medicine (Maywood, N.J.), 242(13), 1345-1354. . ID: 28581337.
21.) Galloway CA, Lee H, Brookes PS, Yoon Y (2014 Sep 15). Decreasing mitochondrial fission alleviates hepatic steatosis in a murine model of nonalcoholic fatty liver disease. American journal of physiology. Gastrointestinal and liver physiology, 307(6), G632-41. . ID: 25080922.
22.) Galloway CA, Lee H, Nejjar S, Jhun BS, Yu T, Hsu W, Yoon Y (2012 Aug). Transgenic control of mitochondrial fission induces mitochondrial uncoupling and relieves diabetic oxidative stress. Diabetes, 61(8), 2093-104. . ID: 22698920.
23.) de Mesy Bentley KL, MacDonald A, Schwarz EM, Oh I (2018 Jan-Mar). Chronic Osteomyelitis with Staphylococcus aureus Deformation in Submicron Canaliculi of Osteocytes: A Case Report. JBJS case connector, 8(1), e8. . ID: 29443819.
The Electron Microscopy Resource supports ultra-structural interrogation of tissues, biologic solutions and nanomaterials for investigators utilizing transmission electron microscopy (TEM), scanning electron microscopy (SEM) and cryo-TEM.
