University of Pittsburgh
Contact Info:
William MacDonald
4401 Penn Ave.
Rangos Research Center Rm 8145
Pittsburgh, PA 15224
Grants and Identifiers:
RRID: RRID:SCR_023116
Instrumentation:
Agilent 4200 TapeStation System
TapeStation System is used to analyze DNA and RNA samples for quality control. [Product Link]
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Agilent 5400 Fragment Analyzer System
Fragment Analyzer System uses automated parallel capillary electrophoresis to redefine bounds of nucleic acid QC analysis [Product Link]
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Illumina MiSeq System
Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths. [Product Link]
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Illumina NextSeq 1000 and NextSeq 2000 Sequencing Systems
Benchtop sequencers that allow you to explore new science across variety of current and emerging applications, with higher efficiency and fewer restraints. [Product Link]
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Illumina NextSeq 550 System
With tunable output and high data quality, it provides flexible power you need for whole genome, transcriptome, and targeted resequencing plus ability to scan microarrays including Infinium MethylationEPIC BeadChip and other select BeadChips. [Product Link]
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(Equipment No Longer Active At this Facility)
Illumina NextSeq 550Dx System
FDA regulated and CE in vitro diagnostic IVD marked, enabling clinical laboratories to develop and perform wide range of applications, from NGS IVD assays using targeted panels, to clinical research applications that include methods from targeted panels to whole genomes. [Product Link]
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Nanodrop Qubit 3 Fluorometer
Benchtop fluorometer that can be used for the quantitation of DNA, RNA, microRNA, and protein using the highly sensitive and accurate fluorescence-based Qubit quantitation assays. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 3.0 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion Personal Genome Machine (PGM) Sequencer. You can also use the Qubit 3.0 Fluorometer to directly measure the fluorescence of samples or to create new assay(s) using the MyQubit software pre-programmed into the instrument. [Product Link]
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Services Provided:
Sequencing - Next Generation Sequencing (NGS)
Relevant Publications:
Description:
The Health Sciences Sequencing Core at UPMC Children’s Hospital of Pittsburgh offers Illumina next generation sequencing services to the University of Pittsburgh research community. Our personnel are experts in next generation sequencing technologies and are committed to remaining abreast of advances that will impact the research effort of investigators. We encourage you to contact our Assistant Director to arrange for a consultation. Our staff is available to share their experience and expertise to assist with development of projects of all sizes, standard or custom assay design, and other specialized application needs.
