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UM Sylvester Onco-Genomics Shared Resource

University of Miami

Contact Info:

Oncogenomics Core Facility (OCF) General Info

Papanicolaou Cancer Research Building, Room 414

1550 N.W. 10th Avenue

Miami, FL 33136

https://www.sylvester.org/OGSR

Grants and Identifiers:

RRID: RRID:SCR_022502

Other Citation Identifiers

P30CA240139

Instrumentation:

10X Genomics Chromium Plus Genetic Analyzer

Genetic analyzer, referred to as Chromium Controller for combining large partition numbers with a diverse barcode library to generate >100,000 barcode-containing partitions. [Product Link]

RRID:SCR_019326

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Agilent 2100 Bioanalyzer Instrument

This Bioanalyzer Instrument is used for sample quality control of biomolecules. [Product Link]

RRID:SCR_019389

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Agilent 5200 Fragment Analyzer System

Fragment Analyzer System allows you to separate 12 samples in parallel in as little as 15 minutes. [Product Link]

RRID:SCR_019417

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Bio-Rad C-1000 Thermal Cycler

Thermal cycler with a touchscreen interface and dual 48 well reaction module that allows multiple independent protocols to be run simultaneously. [Product Link]

RRID:SCR_019688

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Bio-Rad CFX96 Real-Time PCR Detection System

The CFX96 Touch System is a real-time PCR detection system that combines optical technology with temperature control. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, thermal cycler performance, unrivaled stand-alone functionality, and easy-to-use software, the CFX96 Touch System is designed to advance your qPCR. [Product Link]

RRID:SCR_018064

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BioTek Synergy H1 Hybrid Multi-Mode Microplate Reader

Configurable multi mode microplate reader. [Product Link]

RRID:SCR_019748

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Illumina iSeq 100 Sequencing System

Makes next generation sequencing easier. Designed for simplicity, it allows labs of all sizes to sequence DNA and RNA at push of button. [Product Link]

RRID:SCR_020125

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Illumina NovaSeq 6000 Sequencing System

Unleashes groundbreaking innovations that leverage our technology Now you can get scalable throughput and flexibility for virtually any sequencing method, genome, and scale of project. [Product Link]

RRID:SCR_020150

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Miltenyi gentleMACS Dissociator Tissue Dissociator

Benchtop instrument for semi automated dissociation of tissues into single cell suspensions or thorough homogenates. Single sample or two samples in parallel can be processed. [Product Link]

RRID:SCR_020267

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Nanodrop 8000 Spectrophotometer

Microvolume UV Vis spectrophotometer for nucleic acid and protein quantification that can measure multiple samples using 1 to 2 uL of samples. It can analyze spectrum of 220nm to 750nm. [Product Link]

RRID:SCR_018600

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Perkin Elmer Zephy G3 NGS liquid handler

Benchtop liquid handler designed to automate construction of 48 to 96 next generation sequencing libraries per day. [Product Link]

RRID:SCR_018616

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Other Equipment

NextSeq 500 Mission Bio Tapestri Nexcelom K2 cell counter Nanostring Max Covaris CryoPrep CP02

Services Provided:

10x Genomics

Genomics

Nanostring

Nanostring NCounter

Nucleic Acid Extraction

Sequencing - Next Generation Sequencing (NGS)

Single-cell Sequencing

Spatial Transcriptomics

Relevant Publications:

1.) Ortabozkoyun H, Huang PY, Gonzalez-Buendia E, Cho H, Kim SY, Tsirigos A, Mazzoni EO, Reinberg D (2024 Sep 19). Members of an array of zinc-finger proteins specify distinct Hox chromatin boundaries. Molecular cell, 84(18), 3406-3422.e6. . PMID: 39173638.


2.) Manara P, Newsam AD, Saralamma VV, Russo MV, Martinez AB, Fattakhov N, Cunningham TA, Alaoui AY, Chahar D, Carbone AM, Lightfuss OB, Barroso AM, Hoffman KS, Maura F, Bilbao D, Schatz JH (2024 Sep 13). NRF2 translation block by inhibition of cap-dependent initiation sensitizes lymphoma cells to ferroptosis and CAR-T immunotherapy. bioRxiv : the preprint server for biology, (), . . PMID: 39314323.


3.) Kurtenbach S, Sanchez MI, Kuznetsoff J, Rodriguez DA, Weich N, Dollar JJ, Cruz A, Kurtenbach S, Field MG, Durante MA, Decatur C, Sorouri M, Lai F, Yenisehirli G, Fang B, Shiekhattar R, Pelaez D, Correa ZM, Verdun RE, Harbour JW (2024 Feb). PRAME induces genomic instability in uveal melanoma. Oncogene, 43(8), 555-565. . PMID: 38030788.


Description:

The Onco-Genomics Shared Resource (OGSR) provides access to a wide range of technologies and services for the study of the genomics and epigenomics of cancer, in addition to providing technical expertise for project design, trouble-shooting and pre- and post-award support. Principle service areas encompass: 1) next generation sequencing (NGS), 2) single cell genomics 3) Spatial Genomics 4) gene expression assays and molecular quantitation, 5) services for sample extraction and QC.