University of Nebraska Medical Center
Contact Info:
Heather Jensen-Smith
985875 Nebraska Medical Center DRC I 1056
Omaha, NE 68198-5875
https://www.unmc.edu/vcr/cores/vcr-cores/confocal-microscopy/index.html
Grants and Identifiers:
RRID: RRID:SCR_022467
Other Citation IdentifiersP20GM103427 (NIGMS, NE-INBRE)
P30GM106397 (NIGMS, NCCS)
P20GM130447 (NIGMS, CoNDA)
P30CA036727 (NCI, Buffett Cancer Center)
S10RR027301(NIH)
S10OD030486 (NIH)
Nebraska Research Initiative
UNMC Vice Chancellor for Research Office
Instrumentation:
Zeiss LSM 710 Confocal Inverted Microscope
LSM 710 is a confocal is an inverted microscope that enables confocal microscopy for a wide variety of applications. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. With the inverse Axio Observer from Carl Zeiss, LSM 710 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard. [Product Link]
Citation IDs: S10RR027301 (NIH)
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X-Clarity Tissue Clearing System
automated tissue clearing system
Citation IDs: S10OD030486 (NIH)
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Indica Lab HALO Image Analysis Workstation
2D image analysis workstation
Citation IDs: P20GM103427 (NIGMS, NE-INBRE)
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Miltenyi Biotec Ultramicroscope II Light Sheet fluorescence microscope
Lightsheet microscope extending fluorescent imaging into true 3D, large-scale volumetric imaging of intact tissues, organs, and small organisms.
Citation IDs: S10OD030486 (NIH),P20GM130447 (NIGMS, CoNDA)
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Oxford/Andor IMARIS 3D image analysis workstation
3D image analysis workstation
Citation IDs: S10OD030486 (NIH)
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Zeiss 800 CLSM with Airyscan
Inverted microscope dramatically increasing conventional confocal image resolution to ~180 nm using Airyscan technology.
Citation IDs: P30GM106397 (NIGMS, NCCS)
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Zeiss Axioscan 7
a high-performance whole slide scanning system for fluorescence and brightfield imaging.
Citation IDs: P20GM103427 (NIGMS, NE-INBRE)
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Zeiss Celldiscoverer 7
widefield imaging system for automated, time-lapse imaging of live samples.
Citation IDs: P30GM106397 (NIGMS, NCCS),P20GM103427 (NIGMS, NE-INBRE)
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Zeiss ELYRA PS.1
Super Resolution Microscope
Citation IDs: P20GM103427 (NIGMS, NE-INBRE)
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Other Equipment
Zeiss LSM 800 with Airyscan Confocal Inverted Microscope. Zeiss Elyra PS.1 Super Resolution Inverted Microscope Zeiss Cell Discoverer7 Zeiss Axioscan7 Whole Slide Imager Miltenyi Ultramicroscope II Light Sheet Fluorescence Microscope HALO-Equipped Image Analysis Workstation IMARIS-Equipped Image Analysis Workstation
Services Provided:
3D & 4D Image Processing Stations
Confocal/ Super Resolution Microscope
Data Analysis And Interpretation
Fluorescence Microscope With Optical Sectioning Capability
Relevant Publications:
Description:
The Advanced Microscopy Core Facility (AMCF) at UNMC houses state-of-the-art imaging technologies ranging from super-resolution (~ 0.120 um to 0.020 um) to microscopic (~ 0.300 um) to mesoscopic (~ 1 um) biomedical imaging. Imaging specialists provide training and/or actively assist researchers collecting images across the diverse array of imaging instrumentation available in the AMCF with competitively priced usage fees for internal and external facility users. The Zeiss ELYRA PS.1 is an inverted microscope for super-resolution (SR) structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) including, PhotoActivated Localization Microscopy (PALM) using photo-switchable/convertible fluorescent proteins, Total Internal Reflection Fluorescence (TIRF) and STochastic Optical Reconstruction Microscopy (STORM). The Zeiss 800 CLSM with Airyscan (Funding: NIH P30 GM106397) is an inverted microscope dramatically increasing conventional confocal image resolution to ~180 nm using Airyscan technology. The Zeiss 710 LSM (Funding: NIH S10 RR027301) is an inverted microscope supporting most basic imaging applications, multi-channel and spectral, co-localization, live cell, 3D, and time series imaging. The Zeiss Celldiscoverer 7 (Funding: NIH P30 GM106397) is a widefield imaging system for automated, time-lapse imaging of live samples. The Zeiss Axioscan 7 (Funding: NIH P20 GM103427) is a high-performance whole slide scanning system for fluorescence and brightfield imaging. The Miltenyi Biotec Ultramicroscope II Light Sheet fluorescence microscope (Funding: NIH S10 OD030486) extends fluorescent imaging into true 3D, large-scale volumetric imaging of intact tissues, organs, and small organisms. The AMCF also houses several high-end data analysis workstations with premier image analysis software including HALO (Funding: NIH P20 GM103427) and IMARIS (Funding: NIH S10 OD030486) facilitating data rendering, analyses, and presentation options.
