University of Nebraska Medical Center

Contact Info:

Heather Jensen-Smith

985875 Nebraska Medical Center DRC I 1056

Omaha, NE 68198-5875

https://www.unmc.edu/vcr/cores/vcr-cores/confocal-microscopy/index.html

Grants and Identifiers:

RRID: RRID:SCR_022467

P20GM103427 (NIGMS, NE-INBRE)

P30GM106397 (NIGMS, NCCS)

P20GM130447 (NIGMS, CoNDA)

P30CA036727 (NCI, Buffett Cancer Center)

S10RR027301(NIH)

S10OD030486 (NIH)

Nebraska Research Initiative

UNMC Vice Chancellor for Research Office

Instrumentation:

Other Equipment

Zeiss LSM 800 with Airyscan Confocal Inverted Microscope. Zeiss Elyra PS.1 Super Resolution Inverted Microscope Zeiss Cell Discoverer7 Zeiss Axioscan7 Whole Slide Imager Miltenyi Ultramicroscope II Light Sheet Fluorescence Microscope HALO-Equipped Image Analysis Workstation IMARIS-Equipped Image Analysis Workstation

Services Provided:

3D & 4D Image Processing Stations

3D Visualization

Analysis Workstations

Cell Imaging

Confocal Microscopy

Confocal/ Super Resolution Microscope

Consultation

Data Analysis

Data Analysis And Interpretation

Deconvolution

F.I.S.H.

Fluorescence Microscope With Optical Sectioning Capability

Fluorescence Spectroscopy

High-throughput Screening

Image Aquisition

Image Processing And Analysis

Immunofluorescence

Light-sheet Microscopy

Live Cell Imaging

Microscopy

Scanning 20X Brightfield

Scanning 20X Fluorescent

Scanning 40X Brightfield

Scanning 40X Fluorescent

Single-molecule Imaging

Slide Scanning

Spatial Transcriptomics

Super-resolution Microscopy

TIRF Microscopy

Training

Whole Slide Imaging

Relevant Publications:

1.) Jogdeo CM, Panja S, Kumari N, Tang W, Kapoor E, Siddhanta K, Das A, Boesen EI, Foster KW, Oupický D (2024 Dec). Inulin-based nanoparticles for targeted siRNA delivery in acute kidney injury. Journal of controlled release : official journal of the Controlled Release Society, 376(), 577-592. doi: 10.1016/j.jconrel.2024.10.027. PMID: 39419450.

2.) Muehlebach ME, Haney SL, Chhonker YS, Rashid M, Murry DJ, Talmon G, Holstein SA (2024 Oct 23). Geranylgeranyl diphosphate synthase inhibition impairs osteoclast differentiation, morphology, and resorptive activity JBMR Plus, 9(1), ziae133. . PMCID: 11653010.

3.) Zallocchi M, Vijayakumar S, Fleegel J, Batalkina L, Brunette KE, Shukal D, Chen Z, Devuyst O, Liu H, He DZZ, Imami AS, Hamoud AA, McCullumsmith R, Conda-Sheridan M, De Campos LJ, Zuo J (2024 Aug 7). Piplartine attenuates aminoglycoside-induced TRPV1 activity and protects from hearing loss in mice. Science translational medicine, 16(759), eadn2140. . PMID: 39110778.

4.) Clark KL, Shukla M, George JW, Gustin S, Rowley MJ, Davis JS (2024 Jun 26). An environmentally relevant mixture of per- and polyfluoroalkyl substances (PFAS) impacts proliferation, steroid hormone synthesis, and gene transcription in primary human granulosa cells. Toxicological sciences : an official journal of the Society of Toxicology, 200(1), 57-69. . PMID: 38603627.

5.) Krishnan MA, Alimi OA, Pan T, Kuss M, Korade Z, Hu G, Liu B, Duan B (2024 Jan 12). Engineering Neurotoxin-Functionalized Exosomes for Targeted Delivery to the Peripheral Nervous System Pharmaceutics, 16(1), 102. . PMCID: 10818718.

6.) Kabakci R, Clark KL, Plewes MR, Monaco CF, Davis JS (2023 Dec 1). Perfluorooctanoic acid (PFOA) inhibits steroidogenesis and mitochondrial function in bovine granulosa cells in vitro. Environmental pollution (Barking, Essex : 1987), 338(), 122698. . PMID: 37832777.

7.) Bhattacharya DS, Bapat A, Svechkarev D, Mohs AM (2021 Oct 19). Correction to "Water-Soluble Blue Fluorescent Nonconjugated Polymer Dots from Hyaluronic Acid and Hydrophobic Amino Acids". ACS omega, 6(41), 27598. doi: 10.1021/acsomega.1c05070. ID: 34693181.

8.) Al-Mugotir M, Lovelace JJ, George J, Bessho M, Pal D, Struble L, Kolar C, Rana S, Natarajan A, Bessho T, Borgstahl GEO (2021). Selective killing of homologous recombination-deficient cancer cell lines by inhibitors of the RPA:RAD52 protein-protein interaction. PloS one, 16(3), e0248941. doi: 10.1371/journal.pone.0248941. ID: 33784323.

Description:

The Advanced Microscopy Core Facility (AMCF) at UNMC houses state-of-the-art imaging technologies ranging from super-resolution (~ 0.120 um to 0.020 um) to microscopic (~ 0.300 um) to mesoscopic (~ 1 um) biomedical imaging. Imaging specialists provide training and/or actively assist researchers collecting images across the diverse array of imaging instrumentation available in the AMCF with competitively priced usage fees for internal and external facility users. The Zeiss ELYRA PS.1 is an inverted microscope for super-resolution (SR) structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) including, PhotoActivated Localization Microscopy (PALM) using photo-switchable/convertible fluorescent proteins, Total Internal Reflection Fluorescence (TIRF) and STochastic Optical Reconstruction Microscopy (STORM). The Zeiss 800 CLSM with Airyscan (Funding: NIH P30 GM106397) is an inverted microscope dramatically increasing conventional confocal image resolution to ~180 nm using Airyscan technology. The Zeiss 710 LSM (Funding: NIH S10 RR027301) is an inverted microscope supporting most basic imaging applications, multi-channel and spectral, co-localization, live cell, 3D, and time series imaging. The Zeiss Celldiscoverer 7 (Funding: NIH P30 GM106397) is a widefield imaging system for automated, time-lapse imaging of live samples. The Zeiss Axioscan 7 (Funding: NIH P20 GM103427) is a high-performance whole slide scanning system for fluorescence and brightfield imaging. The Miltenyi Biotec Ultramicroscope II Light Sheet fluorescence microscope (Funding: NIH S10 OD030486) extends fluorescent imaging into true 3D, large-scale volumetric imaging of intact tissues, organs, and small organisms. The AMCF also houses several high-end data analysis workstations with premier image analysis software including HALO (Funding: NIH P20 GM103427) and IMARIS (Funding: NIH S10 OD030486) facilitating data rendering, analyses, and presentation options.