Mayo Clinic in Florida
Contact Info:
Oksana Kosiuk
Mayo Clinic
200 First Street SW
Rochester, MN 55905
https://www.mayo.edu/research/core-resources/immunochemical-core/overview
Grants and Identifiers:
RRID: RRID:SCR_017867
Instrumentation:
API 5000 Triple Quadrupole Liquid Chromatography Mass Spectrometer System
Triple quadrupole liquid chromatography mass spectrometer for analysis of bioanalytical samples. It can be used for DMPK and ADME studies. [Product Link]
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Luminex® 100 or 200™ Flow Cytometry Analyzer System
The Luminex® 100 or 200™ System is a flow cytometry analyzer system for individual labs that performs up to 100 different tests in a single reaction volume and can use either magnetic beads or plain polystyrene beads. Luminex 100 or 200 offers higher sample throughput with a 96-well plate read time of only 45 minutes. [Product Link]
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MESO QuickPlex SQ 120
QuickPlex instrument is the newest multiplexing instrument from MSD, offering access to high-performance, electrochemiluminescence immunoassays. This compact system has been engineered for reliability, ease of use, and low cost of ownership. The combination of rapid read times and the ability to perform multiple, simultaneous tests on a single sample increases productivity, saves sample, and delivers results quickly. The QuickPlex has a wide menu of commercially available assay kits and a full line of components and reagents for developing your own assays. [Product Link]
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Molecular Devices SpectraMax Gemini Microplate Reader
Microplate reader built on Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. This reader's dual monochromators for excitation and emission cover the wavelength selection from 250 to 850 nm. Utilize both top and bottom fluorescence reading for the best optical sensitivity, and read up to four wavelength pairs in endpoint and kinetic measurements. The Gemini EM offers superb accuracy and reproducibility of results, possible with AutoPMT electronics, calibration for comparing RFUs between samples, consistent temperature regulation, programmable Automix, gentle movement, seamless automation, and comprehensive reader validation. Applications for the Gemini EM include enzyme assays, protein quantitation, microbial growth, ELISAs and immunoassays, transporter assays, nucleic acid quantitation, phosphotases/kinases, reporter gene assays, and cell viability, proliferation, and cytotoxicity. [Product Link]
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Packard Cobra 5000 Gamma Counter
Gamma counter available in single, five, or ten detector configurations. It can count RIA tubes micro tubes using adapter. This instrument has dot Matrix Printer, tube racks, and protocol ID tags. [Product Link]
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Roche cobas e 411 analyzer
Fully automated analyzer for immunoassay analysis by Roche. Designed for in vitro assay determinations. Applications including anemia, bone, cardiac and tumor markers, critical care, fertility hormones, and infectious diseases. Available as rack or disk sample handling system. [Product Link]
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Thermo Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer
Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer has increased performance and new options that enhance applications from confident DMPK qual/quan screening studies to characterization of intact monoclonal antibodies. [Product Link]
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UniCel DxI 800 Access Immunoassay System
Access immunoassay system analyzer by Beckman Coulter with standardized test menus, assay protocols, instrument processes and reference ranges. Sample types include serum, plasma, urine, amniotic fluid, and whole blood. [Product Link]
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Other Equipment
Roche cobas c 311 chemistry analyzer Agilent 6490 Triple Quadrupole LC-MS/MS
Services Provided:
Relevant Publications:
Description:
The Immunochemical Core is a specialized facility that provides immunochemical, chemistry and immune testing at an affordable cost to clinical and basic science researchers, develops and validates new assays, and improves current assay methodology. Services include: •Extensive test menu (see facility website) •Assay development •Automated chemistry analysis •Immunoassay analysis •Small molecule analysis •Mass Spectrometry, LC-MS/MS and HRAM LC-MS, both high-flow and nano-flow •Enzyme-linked immunosorbent assays (ELISA), Meso Scale Discovery (MSD), Luminex, and radioimmunoassay (RIA) procedures for various analytes •Ability to perform most commercially available assays or kits •Expertise in quantitation of peptides, steroids, hormone, bile acids, sugars, other small molecules
