primary_duns:
878648294
975 NE 10th street
BRC1471
Oklahoma City, OK 73104
United Stateshttps://www.ouhealth.com/stephenson-cancer-center/cancer-research/cancer-shared-resources-services/tissue-pathology-biospecimen-shared-resource/cite this facility
Muralidharan Jayaraman
0000-0003-3274-877XThe goal of the Cancer Tissue Pathology Core is to provide high-quality tissue processing, histology and staining services to SCC members and other investigators. The Core provides tissue processing, embedding, sectioning, histochemical staining of mounted slides, immunohistochemical (IHC) staining for paraffin embedded and frozen tissues, immunocytochemical (ICC) staining for cultured cells (as tissue sections or cytospin slides), evaluation of new antibodies for IHC staining, enzyme histochemistry and special staining. The Core also provides defined analyses including RNA / DNA preparation, reverse transcription and cDNA synthesis from total RNA, construction, staining and analysis of tissue microarrays, and construction and analysis of reverse proteomics array from user-defined biospecimens. The Core is flexible to accommodate the development of new techniques and expanding its services based on the research requirements of SCC members and other investigators.
is_active:
Yesbudget_end:
06/30/2026date_added:
08/23/2025agency_code:
NIHfiscal_year:
2025project_num:
5P30GM154635-02award_amount:
$317,460.00budget_start:
07/01/2025org_name:
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTRorg_state:
OKorg_country:
UNITED STATESorg_zipcode:
731043609primary_duns:
878648294abstract_text:
Abstract The Cell and Tissue Analysis (CTA) Core, a pivotal component of COBRE Phase 3, pioneers molecular, cellular, and tissue analysis technologies, advancing our understanding of cancer biology, therapeutics, and diagnostics. Serving as a central hub, the CTA Core extends its multifaceted services, expertise, and advanced instrumentation to COBRE investigators as well as other cancer researchers in Oklahoma. Under the leadership of Dr. Muralidharan Jayaraman, a seasoned cancer researcher serving as Director, and Dr. Kar-Ming Fung, an accomplished Core Pathologist as Co-director, the CTA Core is dedicated to scientific innovation. Core services include: 1. Experimental Design for Cell and Tissue Analysis: The CTA Core will closely collaborate with researchers to optimize cancer sample analysis, selecting techniques, sample preparation, and statistical approaches. It will ensure meticulous planning for robust, meaningful results and provide guidance on integrating advanced technologies for enhanced cellular and tissue-level investigations in cancer research. 2. Comprehensive Cell and Tissue Analysis: The CTA Core offers precision tissue microarray (TMA) construction and analysis, histochemical staining for mounted slides, comprehensive immunohistochemical (IHC) staining for paraffin-embedded and frozen tissues, immunocytochemical (ICC) staining for cultured cells, various staining techniques, as well as RNAscope- and BaseScope-, and miRNAscope-based in situ hybridization analysis. Additionally, the Core excels in empowering PIs with cutting-edge cell-based analysis, including the isolation and characterization of circulating tumor cells, metabolic flux analysis using Seahorse technology, and facilitating analyses within specialized hypoxic chambers. 3. Cell Authentication and Mycoplasma Testing: Upholding research integrity, the Core offers stringent Short Tandem Repeat (STR) profiling-based cell authentication and mycoplasma testing services, aligning with industry gold standards. As the only facility of its kind in the state of Oklahoma, established in 2012 during Phase I of this COBRE, the CTA Core has played a critical role in meeting the scientific needs of COBRE investigators and other cancer researchers in the region. A strategic financial plan ensures the seamless integration of the Core into the Stephenson Cancer Center (SCC) Research Core Facilities network upon the culmination of Phase 3 COBRE. Throughout Phase 3, the CTA Core will continue to drive cutting-edge cancer research, poised for groundbreaking discoveries in Oklahoma and beyond.project_title:
Cell and Tissue Analysis Corecontact_pi_name:
JAYARAMAN, MURALIDHARANtotal_cost:
$317,460.00is_active:
Nobudget_end:
04/30/2026date_added:
06/28/2025agency_code:
NIHfiscal_year:
2025project_num:
5P30CA225520-08award_amount:
$128,749.00budget_start:
05/01/2025org_name:
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTRorg_state:
OKorg_country:
UNITED STATESorg_zipcode:
731043609primary_duns:
878648294abstract_text:
TISSUE PATHOLOGY AND BIOSPECIMEN SHARED RESOURCE: ABSTRACT The Tissue Pathology and Biospecimen Shared Resource (TPB SR) provides histology services and procures, banks, and distributes human cancer and control tissue and fluid samples. The TPB SR is directed by Kar- Ming Fung, MD, PhD (CT), a pathologist who also serves as a liaison between SCC researchers and other pathologists. During the reporting period, the TPB SR completed a successful integration of the pre-existing SCC biospecimen repository into the TPB SR with a newly established annotated database and an online catalog. Other accomplishments include a new NIH/OD S10 grant and three local grants to establish fluorescence-based whole-slide scanning with image analysis and to expand the capabilities in automated in situ hybridization. To ensure quality and specialization, the TPB SR is organized into two arms. The tissue pathology arm provides: 1) consultation on experimental design and assistance in histopathologic interpretation; 2) a complete line of histology services from tissue processing, embedding, and sectioning paraffin and frozen bocks to construction of tissue / cell block microarrays; 3) automated single or multiplex immunohistochemistry, immunofluorescence, in situ hybridization and TUNEL using chromogen- or fluorescence-based detection; 4) bright-field and fluorescence whole-slide scanning with image analysis; and 5) isolation of circulating tumor cells and laser capture microscopy. The biospecimen arm provides: 1) procurement and banking services for fresh and fixed specimens with an annotated database; 2) an online collection catalog on the institutional intranet with a dash-board type display; and 3) search and retrieval of archival materials from the Department of Pathology. The website and data are stored in electronically and physically separated computers to prevent breach of information. Both arms are housed in SCC-designated space in the OUHSC’s laboratory research complex (BRC & BMSB), a convenient location for a majority (52 of 63 total) lab-based SCC members. From 2017 to 2021, usage by Cancer Biology (CB), Cancer Therapeutics (CT), and Cancer Prevention and Control (CPC) program members was 58%, 36% and 6%, respectively. During this period, SCC members (full and associate) accounted for 60% of total SR usage. SCC members with peer-reviewed support accounted for more than half (52%) of total SR usage, and 87% of SCC member usage. SCC affiliate members (i.e., non-CCSG members who conduct cancer-related work) accounted for another 20% of total usage. Data generated by the TPB SR was used to secure 11 peer-reviewed funded cancer research grants as well as many other grants and scientific publications. TPB SR services are offered free-of-charge to SCC members. This waiver of fees is approved on an annual basis by the SCC director and provides a significant value-added benefit for SCC members to foster research.project_title:
Tissue Pathology and Biospecimen Shared Resourcecontact_pi_name:
FUNG, KAR-MINGtotal_cost:
$128,749.00Services are not offerred outside of
Consulting is not offerred outside of University of Oklahoma Health Sciences Center
Last Updated: 05/13/2026
