University of Pennsylvania

Contact Info:

Luca Musante

https://www.vet.upenn.edu/research/core-resources-facilities/extracellular-vesicle-core

Grants and Identifiers:

RRID: RRID:SCR_022444

Instrumentation:

Services Provided:

Relevant Publications:

Description:

Provides comprehensive or selected services in necessary isolation, quantification and characterization of EVs. Isolation of EV is based on size exclusion using high-performance (SEC-HPLC) or gravity fed (e.g. iZon column) liquid chromatography, ultracentrifugation, and/or density gradient ultracentrifugation. Accurately characterizes EV particle size distribution and concentration using resistive pulse sensing techniques (nCS1, Spectradyne, LLC). Immunophenotype can be accomplished using nanoscale flow cytometry and/or chip array (ExoViewTM) techniques. Provides services in training and education for individuals and lab groups in all methods above and study design consultation.