University of Pennsylvania

Contact Info:

Luca Musante

https://www.vet.upenn.edu/research/core-resources-facilities/extracellular-vesicle-core

Grants and Identifiers:

RRID: RRID:SCR_022444

Instrumentation:

Services Provided:

Relevant Publications:

1.) Palumbos SD, Popolow J, Goldsmith J, Holzbaur EL (2024 Nov 7). Autophagic stress activates distinct compensatory secretory pathways in neurons bioRxiv, (), 2024.11.07.621551. . PMCID: 11580983.

2.) Gasser MT, Liu A, Altamia M, Brensinger BR, Brewer SL, Flatau R, Hancock ER, Preheim SP, Filone CM, Distel DL (2024 Sep 8). Membrane vesicles can contribute to cellulose degradation by Teredinibacter turnerae, a cultivable intracellular endosymbiont of shipworms. bioRxiv : the preprint server for biology, (), . . PMID: 38585906.

Description:

Provides comprehensive or selected services in necessary isolation, quantification and characterization of EVs. Isolation of EV is based on size exclusion using high-performance (SEC-HPLC) or gravity fed (e.g. iZon column) liquid chromatography, ultracentrifugation, and/or density gradient ultracentrifugation. Accurately characterizes EV particle size distribution and concentration using resistive pulse sensing techniques (nCS1, Spectradyne, LLC). Immunophenotype can be accomplished using nanoscale flow cytometry and/or chip array (ExoViewTM) techniques. Provides services in training and education for individuals and lab groups in all methods above and study design consultation.