University of Pennsylvania

Contact Info:

Vera Moiseenkova-Bell

https://www.med.upenn.edu/electronmicroscopyresourcelab/

Grants and Identifiers:

RRID: RRID:SCR_022375

Instrumentation:

Services Provided:

Relevant Publications:

1.) Fenton AR, Peng R, Bond C, Hugelier S, Lakadamyali M, Chang YW, Holzbaur EL, Jongens TA (2024 Nov 15). FMRP regulates MFF translation to locally direct mitochondrial fission in neurons Nature Cell Biology, 26(12), 2061-2074. . PMCID: 11628401.

2.) Liu X, Jean-Gilles R, Baginski J, Cai C, Yan R, Zhang L, Lance K, van der Loo JC, Davidson BL (2024 Jul 15). Evaluation of a rapid multi-attribute combinatorial high-throughput UV-Vis/DLS/SLS analytical platform for rAAV quantification and characterization Molecular Therapy. Methods & Clinical Development, 32(3), 101298. . PMCID: 11338085.

3.) Vrettos N, Oppelt J, Zoch A, Sgourdou P, Yoshida H, Song B, Fink R, O’Carroll D, Mourelatos Z (2024 Mar 23). MIWI N-terminal arginines orchestrate generation of functional pachytene piRNAs and spermiogenesis Nucleic Acids Research, 52(11), 6558-6570. . PMCID: 11194079.

Description:

Research core is organized into three interdependent facilities. Ultrastructure lab offers training to Standard-EM and Immuno-EM for cellular ultra-structures of tissue samples. Cryo-EM Screening and Training offers training on Microscopes and ancillary equipment for Negative Stain Imaging, Single-Particle Cryo-EM and Cryo-Tomography of large complexes and cells. Beckman Center for Cryo-EM offers service for high resolution data collection of prescreened and well qualified samples for single particle analysis and Tomography using Titan Krios microscope with Gatan K3 camera.