University of Mississippi
Contact Info:
Kristin Edwards
Grants and Identifiers:
RRID: RRID:SCR_028584
Instrumentation:
Services Provided:
Relevant Publications:
Description:
Core provides specialized expertise, instrumentation, and technical support for assessment of mitochondrial function in cells, tissues, and isolated mitochondria. Provides standardized mitochondrial isolation, experimental design consultation, assay optimization, data analysis, and interpretation. Services include high-resolution respirometry using the Oroboros O2k-FluoRespirometer, simultaneous measurement of oxygen consumption and reactive oxygen species production, mitochondrial respiratory control and coupling analyses, electron transport system complex activity assays, citrate synthase activity, mitochondrial calcium handling, and assessment of mitochondrial content and oxidative stress. Studies can be performed using isolated mitochondria, permeabilized tissue fibers, tissue homogenates, and intact cells, depending on the scientific question and sample availability. Core also assists with protocol development, selection of normalization strategies, troubleshooting, training, and preparation of methods and data for grants, presentations, and publications.
