The Ohio State University
Contact Info:
Wirat Pipatpongpinyo
1680 Madison Ave.,
Wooster, OH 44691
Grants and Identifiers:
RRID: RRID:SCR_028289
Instrumentation:
Agilent 4200 Tapestation Genetic Analyzer
Benchtop genetic analyzer electrophoresis system for DNA and RNA sample quality control. It can be used for next generation sequencing or Biobank workflow. [Product Link]
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Applied Biosystems SeqStudio Genetic Analyzer
SeqStudio Genetic Analyzer is fluorescent dye labeled genetic analysis system using capillary electrophoresis technology. Enables both sequencing and fragment analysis applications without the need to switch polymer type or capillary array length. Allows to perform both sequencing and fragment analysis in single run. [Product Link]
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Bio Rad ChemiDoc MP Imaging System
Instrument for imaging and analyzing gels and western blots by Bio Rad. Designed to address multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs. [Product Link]
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Bio-Rad QX200 Droplet Digital PCR System
Droplet generator and reader that provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. [Product Link]
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Eppendorf 5075 epMotion Automated Liquid Handling System
System provides precise, accurate alternative to time-consuming manual pipetting and liquid handling tasks. The combination of accelerated operational speed, new software & hardware features and an increased number of worktable positions make the systems best for and flexible laboratory use across a wide range of liquid handling applications. To ensure easy and consistent use, epMotion 5075 employs both Eppendorf's established epBlue software and an new software assistant concept. This supports the programming of applications and ensures that all users can use the system with only initial training. In addition, novel features such as a new CleanCap option, including UV light decontamination and a HEPA filter for purified air, offer new standards of decontamination and sample safety. This is supported even further by an optical sensor that checks labware, liquid levels and tip counts and type at the start of each cycle, hugely decreasing any risk of error during the run. [Product Link]
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(Equipment No Longer Active At this Facility)
Fluidigm C1 C1
Automated system that carefully isolates single cells into individual reaction chambers in the exclusive Fluidigm integrated fluidic circuit (IFC). C1 lets you prepare single-cell templates for mRNA sequencing, DNA sequencing, epigenetics or miRNA expression. You can extract, reverse transcribe, amplify, and ultimately detect and analyze gene expression using just one technology, eliminating the added variability introduced by human error and multi-platform approaches. With an optimized protocol, pre-formulated reagent kits, and disposable IFCs, users can achieve single-cell accuracy and a cDNA product that is ready for use with the Epigenetics Core's Biomark HD high throughput PCR system. [Product Link]
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Illumina MiSeq System
Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths. [Product Link]
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Illumina NextSeq 1000 and NextSeq 2000 Sequencing Systems
Benchtop sequencers that allow you to explore new science across variety of current and emerging applications, with higher efficiency and fewer restraints. [Product Link]
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Labconco CentriVap Refrigerated Centrifugal Concentrator
Refrigerated centrifugal concentrator that concentrates heat sensitive samples using centrifugal force, vacuum, and controlled temperature. Concentrator has heater and refrigeration systems have temperature range of negative 4 C to 100 C. [Product Link]
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MinION
Portable, real time device for DNA and RNA sequencing, putting you in control of your sequence data. Portable DNA/RNA sequencing by Oxford Nanopore Technologies. [Product Link]
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Nanodrop 8000 Spectrophotometer
Microvolume UV Vis spectrophotometer for nucleic acid and protein quantification that can measure multiple samples using 1 to 2 uL of samples. It can analyze spectrum of 220nm to 750nm. [Product Link]
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Qiagen QIAcube DNA/RNA Protein Purification System
Protein purification system that performs fully automated purification of nucleic acids and proteins in molecular biology applications. [Product Link]
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Thermo Fisher Qubit 2.0 Fluorometer Qubit 2.0
Invitrogen Qubit Flex Fluorometer can selectively, accurately measure the concentration of up to 8 samples of DNA, RNA or protein simultaneously. Compared to single sample microvolume fluorometers, using the Qubit Flex Fluorometer for multiple samples reduces your time to data by up to 50%, with time savings beginning with batches of as few as 8 samples. Combining the one-of-a-kind Qubit Flex Fluorometer and optimized Invitrogen Qubit reagents and tube strips offers a complete solution for DNA, RNA and protein quantification. [Product Link]
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Thermo Scientific Nanodrop 1000 Spectrophotometer
The Thermo Scientific NanoDrop™ 1000 is a spectrophotometer that performs measuring of 1 ul samples with high accuracy and reproducibility. The full spectrum (220nm-750nm) spectrophotometer utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. In addition, the NanoDrop 1000 Spectrophotometer has the capability to measure highly concentrated samples without dilution (50X higher concentration than the samples measured by a standard cuvette spectrophotometer). [Product Link]
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Services Provided:
Illumina RNA/DNA NGS Library Construction
Long Read Sequencing Utilizing Oxford Nanopore’s MinION Platform
Nucleic Acid Quantitation (TapeStation, Qubit)
Sequencing - Next Generation Sequencing (NGS)
Sequencing- Long Read Sequencing
Relevant Publications:
Description:
Core provides integrated, end-to-end support for molecular biology and genomics research. Services include DNA/RNA extraction, sample quality control, genotyping, qPCR/ddPCR, and next-generation sequencing using Illumina and Oxford Nanopore platforms. In addition to laboratory services, MBGC offers consultation, experimental design guidance, troubleshooting, and training to support high-quality and reproducible research outcomes.
