Medical College of Wisconsin
John J. Reho
8701 Watertown Plank Road
Milwaukee, WI 53222
https://mcw.ilab.agilent.com/service_center/5692/?tab=about
RRID: RRID:SCR_028283
Sable Systems International Promethion
Tecniplast Metabolic Caging
Bioimpedance Spectroscopy With ImpediVet
Body Composition Analysis With LF110 NMR (Bruker Biospin)
Body Composition And Bone Analysis With Dual-Energy X-ray Absorptiometry
Bomb Calorimetry (Parr Instruments)
Metabolic Caging (Tecniplast)
Metabolic Phenotyping With 16-cage Promethion Core (Sable Systems)
Soft Tissue And Bone Analysis With Insight Vet DEXA (Osteosys)
Stand-alone Respirometry With FMS-3 Respirometers (Sable Systems)
Thermal Imaging With FLIR A700 Camera
1.) Ke X, Grobe CC, Grobe JL, Yu W, Reho JJ, Varberg KM, Lane RH, Segar JL (2026 Mar 28). Maternal Low Sodium Diet Impairs Hippocampal Neurogenesis and Cognition in Adult Mouse Offspring. American journal of physiology. Regulatory, integrative and comparative physiology, (), . doi: 10.1152/ajpregu.00304.2025. ID: 41902531.
2.) Yunker L, Usa K, Williams AC, Reho JJ, Grobe JL, Gumz ML, Kriegel AJ (2025 Oct). Time-Restricted Feeding Attenuates Salt-Sensitive Hypertension and Renal Damage. Hypertension (Dallas, Tex. : 1979), 82(10), 1743-1753. . ID: 40765526.
3.) Pasos J, Wagner VA, Lawton SBR, Madison AM, Nandani K, Mathieu NM, Grobe CC, Reho JJ, Freudinger BP, Sherman AJ, Brozoski DT, Morselli LL, Burnett CML, Segar JL, Nakagawa P, Sigmund CD, Grobe JL (2025 Jul 18). Angiotensin AT(1)R expressing cells within the ventral hypothalamus modulate integrative control of cardiometabolic functions. iScience, 28(7), 112797. . ID: 40585361.
4.) Hadiono MA, Kazen AB, Aboulalazm FA, Burnett CML, Reho JJ, Kindel TL, Grobe JL, Kirby JR (2025 Jun 1). Reutericyclin mitigates risperidone-induced suppression of anaerobic energy expenditure. American journal of physiology. Regulatory, integrative and comparative physiology, 328(6), R741-R757. . ID: 40235074.
5.) Wildes MP, Fernando DG, Grobe CC, Reho JJ, Grobe JL, Kidambi S, Kindel TL, Kwitek AE, Segar JL, Williams JS, Morselli LL (2024 Aug 27). Long-term Metabolic Dysfunction Programming in Female Mice by Serial Moderate Restriction of a High-fat High-sucrose Diet. Endocrinology, 165(10), . . ID: 39236000.
6.) Mathieu NM, Tan EE, Reho JJ, Brozoski DT, Muskus PC, Lu KT, Wackman KK, Grobe JL, Nakagawa P, Sigmund CD (2024 Jun). Genetic Deletion of β-Arrestin 2 From the Subfornical Organ and Other Periventricular Nuclei in the Brain Alters Fluid Homeostasis and Blood Pressure. Hypertension (Dallas, Tex. : 1979), 81(6), 1332-1344. . ID: 38629290.
7.) Ritter ML, Wagner VA, Balapattabi K, Opichka MA, Lu KT, Wackman KK, Reho JJ, Keen HL, Kwitek AE, Morselli LL, Geurts AM, Sigmund CD, Grobe JL (2024 Mar 1). Krüppel-like factor 4 in transcriptional control of the three unique isoforms of Agouti-related peptide in mice. Physiological genomics, 56(3), 265-275. . ID: 38145289.
8.) Lawton SB, Grobe CC, Reho JJ, Raff H, Thulin JD, Jensen ES, Burnett CM, Segar JL, Grobe JL (2024 Mar 1). Differences in Fluid, Electrolyte, and Energy Balance in C57BL/6J Mice (Mus musculus) in Metabolic Caging at Thermoneutral or Standard Room Temperatures. Journal of the American Association for Laboratory Animal Science : JAALAS, 63(2), 190-200. . ID: 38191147.
9.) Reho JJ, Muskus PC, Bennett DM, Grobe CC, Burnett CML, Nakagawa P, Segar JL, Sigmund CD, Grobe JL (2024 Mar 1). Modulatory effects of estrous cycle on ingestive behaviors and energy balance in young adult C57BL/6J mice maintained on a phytoestrogen-free diet. American journal of physiology. Regulatory, integrative and comparative physiology, 326(3), R242-R253. . ID: 38284128.
10.) Wagner VA, Holl KL, Clark KC, Reho JJ, Lehmler HJ, Wang K, Grobe JL, Dwinell MR, Raff H, Kwitek AE (2023 Nov 2). The Power of the Heterogeneous Stock Rat Founder Strains in Modeling Metabolic Disease. Endocrinology, 164(12), . . ID: 37882530.
11.) Ziegler AA, Lawton SBR, Grobe CC, Reho JJ, Freudinger BP, Burnett CML, Nakagawa P, Grobe JL, Segar JL (2023 Nov 1). Early-life sodium deprivation programs long-term changes in ingestive behaviors and energy expenditure in C57BL/6J mice. American journal of physiology. Regulatory, integrative and comparative physiology, 325(5), R576-R592. . ID: 37720996.
12.) Grobe CC, Reho JJ, Brown-Williams D, Ziegler AA, Mathieu NM, Lawton SBR, Fekete EM, Brozoski DT, Wackman KK, Burnett CML, Nakagawa P, Sigmund CD, Segar JL, Grobe JL (2023 Sep). Cardiometabolic Effects of DOCA-Salt in Mice Depend on Ambient Temperature. Hypertension (Dallas, Tex. : 1979), 80(9), 1871-1880. . ID: 37470185.
13.) Balapattabi K, Yavuz Y, Jiang J, Deng G, Mathieu NM, Ritter ML, Opichka MA, Reho JJ, McCorvy JD, Nakagawa P, Morselli LL, Mouradian GC Jr, Atasoy D, Cui H, Hodges MR, Sigmund CD, Grobe JL (2023 Aug 29). Angiotensin AT(1A) receptor signal switching in Agouti-related peptide neurons mediates metabolic rate adaptation during obesity. Cell reports, 42(8), 112935. . ID: 37540598.
14.) Wagner VA, Holl KL, Clark KC, Reho JJ, Dwinell MR, Lehmler HJ, Raff H, Grobe JL, Kwitek AE (2023 Jun 28). Genetic background in the rat affects endocrine and metabolic outcomes of bisphenol F exposure. Toxicological sciences : an official journal of the Society of Toxicology, 194(1), 84-100. . ID: 37191987.
15.) Mathieu NM, Fekete EM, Muskus PC, Brozoski DT, Lu KT, Wackman KK, Gomez J, Fang S, Reho JJ, Grobe CC, Vazirabad I, Mouradian GC Jr, Hodges MR, Segar JL, Grobe JL, Sigmund CD, Nakagawa P (2023). Genetic Ablation of Prorenin Receptor in the Rostral Ventrolateral Medulla Influences Blood Pressure and Hydromineral Balance in Deoxycorticosterone-Salt Hypertension. Function (Oxford, England), 4(5), zqad043. . ID: 37609445.
16.) Ritter ML, Deng G, Reho JJ, Deng Y, Sapouckey SA, Opichka MA, Balapattabi K, Wackman KK, Brozoski DT, Lu KT, Paradee WJ, Gibson-Corley KN, Cui H, Nakagawa P, Morselli LL, Sigmund CD, Grobe JL (2022 Dec). Cardiometabolic Consequences of Deleting the Regulator of G protein Signaling-2 (Rgs2) From Cells Expressing Agouti-Related Peptide or the ANG (Angiotensin) II Type 1A Receptor in Mice. Hypertension (Dallas, Tex. : 1979), 79(12), 2843-2853. . ID: 36259376.
17.) Mathieu NM, Nakagawa P, Grobe CC, Reho JJ, Brozoski DT, Lu KT, Wackman KK, Ritter ML, Segar JL, Grobe JL, Sigmund CD (2022 Nov). ARRB2 (β-Arrestin-2) Deficiency Alters Fluid Homeostasis and Blood Pressure Regulation. Hypertension (Dallas, Tex. : 1979), 79(11), 2480-2492. . ID: 36215165.
18.) Oliveira V, Reho JJ, Balapattabi K, Ritter ML, Mathieu NM, Opichka MA, Lu KT, Grobe CC, Silva SD Jr, Wackman KK, Nakagawa P, Segar JL, Sigmund CD, Grobe JL (2022 Oct 1). Chronic intracerebroventricular infusion of angiotensin II causes dose- and sex-dependent effects on intake behaviors and energy homeostasis in C57BL/6J mice. American journal of physiology. Regulatory, integrative and comparative physiology, 323(4), R410-R421. . ID: 35816717.
19.) Reho JJ, Nakagawa P, Mouradian GC Jr, Grobe CC, Saravia FL, Burnett CML, Kwitek AE, Kirby JR, Segar JL, Hodges MR, Sigmund CD, Grobe JL (2022). Methods for the Comprehensive in vivo Analysis of Energy Flux, Fluid Homeostasis, Blood Pressure, and Ventilatory Function in Rodents. Frontiers in physiology, 13(), 855054. . ID: 35283781.
Core provides guided access to technologies for dissection of metabolic function in rodents and other small animals. Provides development, testing, and standardization of metabolic technologies and workflows to leverage both mouse and rat models of interest to the MCW research community. Offers 16-cage multiplexed metabolic phenotyping system (Promethion, Sable Systems International) that provides detailed mechanistic dissection of resting metabolic rate (RMR), physical activity, and ingestive behaviors of rodents in home style cage designs across the light cycle. Core combines RMR assessments with direct/combined calorimetry (total heat dissipation) to enable assessment of anaerobic forms of energy expenditure. Additional services include body composition analysis, metabolic caging, indirect calorimetry, bioimpedance spectroscopy, thermal imaging. Core experts provide guidance for experimental design, execution of studies, statistical analysis of resulting data, and regulatory assistance and compliance.
