Cancer Research UK

Contact Info:

Clive D’Santos

https://www.cruk.cam.ac.uk/core-facilities/proteomics/

Grants and Identifiers:

RRID: RRID:SCR_028276

Instrumentation:

Services Provided:

Relevant Publications:

Description:

Core offers services for identification, quantification, and structural characterisation of proteins using next–generation mass spectrometry platforms. Uses mass spectrometry platforms that include Orbitrap Lumos, Ascend and Astral Zoom systems. Services include designing of experimental strategies, implementing and validating previously developed proteomic workflows to profile proteins from diverse biological samples, as well as developing methods and assays when required. Core focuses and specializes in using stable isotopic labelling strategies (Tandem Mass Tags) as well as label-free methods using different acquisition methods (DDA and DIA) for relative quantification of protein expression levels and enrichment within protein complexes. Develops and runs PRM (parallel reaction monitoring) assays for targeted quantification of defined panels of proteins.