Memorial Sloan Kettering Cancer Center
Contact Info:
Phillip Wong
417 E 68th Street
New York, NY 10065
https://www.mskcc.org/research/ski/core-facilities/immune-monitoring
Grants and Identifiers:
RRID: RRID:SCR_027800
Instrumentation:
Services Provided:
Antigen-Specific T Cell Assays Via ELISPOT Or IC
Multiparameter Flow Cytometry Assays
Multiplex Cytokine Immunoassays
PBMC Sample Processing And Inventory Management
Relevant Publications:
Description:
The Immune Monitoring Facility (IMF) provides translational research support for immune monitoring during cancer immunotherapy. Robust immune monitoring assays are essential for characterizing the immune status in patients receiving novel immune-modulating therapies, and the insights gained from studying the immune response in treated patients may help transition promising therapies from the clinical trial phase to standard of care. The IMF supports a variety of clinical research activities including standardized processing and cryopreservation of numerous clinical samples on a daily basis from patients on experimental cancer therapies, development and validation of immune monitoring assays, and correlative sample analysis using these assays to evaluate immune responses in patients enrolled in various immunotherapy trials. The facility utilizes highly specialized technologies to analyze both cellular and soluble protein biomarkers with the ultimate goal of identifying biomarkers that correlate with clinical responses which can then guide clinical decisions regarding the patients that may benefit most from specific immunotherapy regimens. Services and assays provided in the facility include, but are not limited to: (1) PBMC and serum/plasma isolation from human whole blood and sample cryopreservation; (2) Multiplex cytokine immunoassays; (3) Immunophenotypic analysis of immune cell subsets by multiparameter flow cytometry; (4) Evaluation of T cell antigen specificity and function by intracellular cytokine staining and ELISPOT assays.
