University of Michigan
Venkatesha Basrur/Kevin Conlon/Yanlong Ji
MSRB2, Rm. 3556
1150 W. Medical Center Dr
Ann Arbor, MI 48109
https://cores.research.umich.edu/core/proteomics-resource-facility/
RRID: RRID:SCR_026723
Thermo Scientific Q Exactive HF Orbitrap LC-MS/MS System
Thermo Exactive benchtop Orbitrap operates with a Waters Acquity UPLC and generates high resolution, high mass accuracy full Mass Spectrometry data. [Product Link]
RRID:SCR_020545
ThermoScientific Orbitrap Ascend with ETD and FAIMS
ThermoScientific Orbitrap Fusion
Crosslinking Mass Spectrometry
Data Independent Acquisition (DIA)
Global PTM Mapping
Protein Interactions (IP/MS, AP/MS, Proximity Labeling, DNA-protein Interactions, Chemoproteomics)
Protein Post-translational Modification (PTM) Identification
Relative Quantitation (TMT Or Other)
Shotgun Proteomics
Targeted Proteomics (PRM)
1.) Mandal J, Aryal S, Manandhar I, Chakraborty S, Mei X, Yeoh BS, Mell B, Kleinhenz A, Tummala R, Yang T, Saha P, Gunning WT 3rd, Vijay-Kumar M, Basrur V, de la Serna I, Joe B (2025 Oct). Epigenetic Histone β-Hydroxybutyrylation Contributes to Renoprotection by β-Hydroxybutyrate in the Dahl Rat. Hypertension (Dallas, Tex. : 1979), 82(10), 1729-1742. doi: 10.1161/HYPERTENSIONAHA.125.25251. ID: 40726393.
2.) Li J, Deng Y, Gasser M, Zhu J, Walker EM, Sidarala V, Reck EC, Hubers DL, Pasmooij MB, Shin CS, Bandesh K, Motakis E, Nargund S, Kursawe R, Basrur V, Nesvizhskii AI, Stitzel ML, Chan DC, Rutter GA, Soleimanpour SA (2025 Aug). LONP1 regulation of mitochondrial protein folding provides insight into beta cell failure in type 2 diabetes. Nature metabolism, 7(8), 1570-1592. . ID: 40691304.
3.) Mohan HM, Fernandez MG, Huang C, Lin R, Ryou JH, Seyfried D, Grotewold N, Barget AJ, Whiteley AM, Basrur V, Mosalaganti S, Paulson HL, Sharkey LM (2025 Jul 18). Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology. Science advances, 11(29), eadu6354. . ID: 40680123.
4.) Zhao HY, Zhang L, Liu Z, He M, Wang M, Li Q, Sarkari F, Tao J, Wen B, Basrur V, Myatt H, Nesvizhskii A, Sun D (2025 Jun 12). Design of Potent Small-Molecule Stimulator of Interferon Gene Inhibitor and Stimulator of Interferon Gene Mutant-Specific Degrader. Journal of medicinal chemistry, 68(11), 11100-11126. doi: 10.1021/acs.jmedchem.5c00123. ID: 40386971.
5.) Sisson TH, Osterholzer JJ, Leung L, Basrur V, Nesvizhskii A, Subbotina N, Warnock M, Torrente D, Virk AQ, Gutor SS, Horowitz JC, Migliorini M, Strickland DK, Kim KK, Huang SK, Lawrence DA (2025 Jun 9). PAI-1 interaction with sortilin-related receptor 1 is required for lung fibrosis. JCI insight, 10(11), . . ID: 40299646.
6.) Underwood M, Da Veiga Leprevost F, Basrur V, Nesvizhskii AI, Rawley O, Golden K, Emmer B, Lillicrap D, Desch K (2025 May). Identification of multiple novel procoagulant plasma ligands for stabilin-2. Journal of thrombosis and haemostasis : JTH, 23(5), 1622-1635. doi: 10.1016/j.jtha.2025.01.023. ID: 39970990.
7.) Sahasrabuddhe AA, Chen X, Ma K, Wu R, Liang HC, Kapoor R, Chhipa RR, Onder O, McFetridge C, Van Arnam JS, Zhang X, Morrissette JJD, Pillai V, Li MM, Szankasi P, Basrur V, Conlon KP, Raabe TD, Bailey NG, Hogaboam CM, Rottapel R, Kim J, López C, Schlesner M, Siebert R, Dreval K, Morin RD, Moro L, Pagano M, Staudt LM, Lim MS, Elenitoba-Johnson KSJ (2025 Apr 2). The FBXO45-GEF-H1 Axis Controls Germinal Center Formation and B-cell Lymphomagenesis. Cancer discovery, 15(4), 838-861. . ID: 39820335.
8.) Clauwaert J, McVey Z, Gupta R, Yannuzzi I, Basrur V, Nesvizhskii AI, Menschaert G, Prensner JR (2025 Feb 2). Deep learning to decode sites of RNA translation in normal and cancerous tissues. Nature communications, 16(1), 1275. . ID: 39894899.
9.) Levi-D (2025 Feb 1). TRAF6 integrates innate immune signals to regulate glucose homeostasis via Parkin-dependent and -independent mitophagy. bioRxiv : the preprint server for biology, (), . . ID: 39974969.
10.) Tang VT, Abbineni PS, Veiga Leprevost FD, Basrur V, Khoriaty R, Emmer BT, Nesvizhskii AI, Ginsburg D (2023 Nov 3). Identification of LMAN1- and SURF4-Dependent Secretory Cargoes. Journal of proteome research, 22(11), 3439-3446. . ID: 37844105.
11.) Kaur A, Surnilla A, Zaitouna AJ, Mumphrey MB, Basrur V, Grigorova I, Cieslik M, Carrington M, Nesvizhskii AI, Raghavan M (2023 Nov 1). Mass Spectrometric Profiling of HLA-B44 Peptidomes Provides Evidence for Tapasin-Mediated Tryptophan Editing. Journal of immunology (Baltimore, Md. : 1950), 211(9), 1298-1307. . ID: 37737643.
12.) Tan Z, Yue Y, Leprevost F, Haynes S, Basrur V, Nesvizhskii AI, Verhey KJ, Cianfrocco MA (2023 Nov 1). Autoinhibited kinesin-1 adopts a hierarchical folding pattern. eLife, 12(), . . ID: 37910016.
13.) Crump NT, Smith AL, Godfrey L, Dopico-Fernandez AM, Denny N, Harman JR, Hamley JC, Jackson NE, Chahrour C, Riva S, Rice S, Kim J, Basrur V, Fermin D, Elenitoba-Johnson K, Roeder RG, Allis CD, Roberts I, Roy A, Geng H, Davies JOJ, Milne TA (2023 Aug 25). MLL-AF4 cooperates with PAF1 and FACT to drive high-density enhancer interactions in leukemia. Nature communications, 14(1), 5208. . ID: 37626123.
14.) Singh AK, Kumar R, Yin J, Brooks Ii JF, Kathania M, Mukherjee S, Kumar J, Conlon KP, Basrur V, Chen Z, Han X, Hooper LV, Burstein E, Venuprasad K (2023 Aug 17). RORγt-Raftlin1 complex regulates the pathogenicity of Th17 cells and colonic inflammation. Nature communications, 14(1), 4972. . ID: 37591835.
Core is dedicated to protein analysis and applying mass spectrometry-based proteomic approaches to varied biological questions.
