Max Planck Institute of Biochemistry
Jerome Basquin
https://www.biochem.mpg.de/protein_production
RRID: RRID:SCR_025741
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Facility for protein production. Provides services for protein expression and purification, assists with projects in designing strategies for high level expression of soluble proteins, provides vectors and strains. Offers clonning the gene of interest into expression vectors, rapid screening for optimal combinations of vectors, strains and expression conditions and producing protein of interest in E. coli, Pichia pastoris, mammalian and insect cells. Produces biomass at 1L to 10L scale using multiple bioreactor system for high cell density fermentation of bacteria and yeast cells and reusable bioreactor for cell culture. Proteins are then purified using standard techniques and chromatography systems. Quality control is routinely assessing protein identity, purity, stability and folding using set of different biochemical and biophysical methods.
