Fred Hutch Cancer Center

Contact Info:

Shared Resources Administration

1100 Fairview Ave N.

DE-352

Seattle, WA 98109

https://www.fredhutch.org/en/research/shared-resources/core-facilities/proteomics-metabolomics.html

Grants and Identifiers:

RRID: RRID:SCR_022618

P30CA015704

Instrumentation:

Other Equipment

Orbitrap Eclipse with FAIMS Mass Spectrometer Orbitrap Fusion with ETD Mass Spectrometer TSQ Vantage

Services Provided:

HPLC

Mass Spectrometry

Mass Spectrometry - Proteins And Peptides

Proteomics

Quantitative Proteomics - TMT

Relevant Publications:

1.) Ling M, Kacherovsky N, Wu AY, Ni M, Hamerman JA, Pun SH (2025 Dec 2). Rapid and label-free isolation of human peripheral blood monocytes using a reversible CD36-binding aptamer for cell capture. Biomaterials science, 13(24), 6805-6817. . PMCID: 12557302.

2.) Wu AY, Cheng EL, Kacherovsky N, Marking A, Lin-Goldstein A, Heinze CM, Salipante SJ, Jensen MC, Pun SH (2025 Aug 29). Efficient and Traceless Aptamer-Based Serial Selection of Naïve and Early Memory CD8 T Cells for CAR T Cell Therapy. Advanced healthcare materials, (), e02930. doi: 10.1002/adhm.202502930. PMID: 40879088.

3.) Nelson CR, Mallett DR, Biggins S (2025 Apr 4). Spindle integrity is regulated by a phospho-dependent interaction between the Ndc80 and Dam1 kinetochore complexes PLOS Genetics, 21(4), e1011645. . PMCID: 12007717.

4.) Briney CA, Henriksen JC, Lin C, Jones LA, Benner L, Rains AB, Gutierrez R, Gafken PR, Rissland OS (2025 Feb 20). Muskelin is a substrate adaptor of the highly regulated Drosophila embryonic CTLH E3 ligase EMBO Reports, 26(6), 1647-1669. . PMCID: 11933467.

5.) Grosely R, Alvarado C, Ivanov IP, Nicholson OB, Puglisi JD, Dever TE, Lapointe CP (2024 Jul 13). eIF1 and eIF5 dynamically control translation start site fidelity bioRxiv, (), 2024.07.10.602410. . PMCID: 11257575.

6.) Briney CA, Henriksen JC, Lin C, Jones LA, Benner L, Rains AB, Gutierrez R, Gafken PR, Rissland OS (2024 Jul 1). Muskelin acts as a substrate receptor of the highly regulated Drosophila CTLH E3 ligase during the maternal-to-zygotic transition bioRxiv, (), 2024.06.28.601265. . PMCID: 11244905.

7.) Davidson KA, Nakamura M, Verboon JM, Parkhurst SM (2023 May 10). Centralspindlin proteins Pavarotti and Tumbleweed along with WASH regulate nuclear envelope budding The Journal of Cell Biology, 222(8), e202211074. . PMCID: 10174194.

Description:

Provides support for mass spectrometry based proteomics and metabolomics research. With advanced instrumentation and highly trained staff, core offers consultation and services in sample preparation, sample separation, data collection and data analysis