Fred Hutch Cancer Center
Shared Resources Administration
1100 Fairview Ave N.
DE-690
Seattle, WA 98109
https://www.fredhutch.org/en/research/shared-resources/core-facilities/flow-cytometry.html
RRID: RRID:SCR_022613
Other Citation IdentifiersP30CA015704
Sony ID 7000F
BD FACSymphony A5
BD LSRFortessa X-50
BD FACSCelesta
BD Canto II
BD FACSymphony S6
BD Aria II
Sony MA900
Miltenyi Biotec MACSQuant Tyto
Fluidigm Helios CyTOF
CyTOF
FACS Cell Sorting
Flow Cytometric Analysis
Flow Cytometry Data Analysis
Mass Spectrometry
1.) Avanessian SC, van den Bijgaart RJ, Chew NW, Supper VM, Tang TT, Zhang Y, Zhao YQ, Abe K, Gauthier J, Barry KC (2025 Oct 13). IL-2/IL-15 signaling induces NK cell production of FLT3LG augmenting anti-PD-1 immunotherapy Cancer immunology research, (), 10.1158/2326-6066.CIR-24-1259. . PMCID: 12582536.
2.) Kuhlmann AS, Madkhali N, Moskovitz E, Parrott JE, Raman SS, Riker AO, Martinez-Reyes J, Gupta M, Jang RA, Nelson V, Gray MD, Taylor JJ, Peterson CW, Kiem HP (2025 Sep 22). Heavy-chain immunoglobulin locus editing in rhesus macaque B cells to confer antibody production Molecular Therapy. Methods & Clinical Development, 33(4), 101598. . PMCID: 12554106.
3.) Granadier D, Cooper K, Acenas D II, Kousa A, Warren M, Hernandez V, Iovino L, deRoos P, Lederer EE, Shannon-Sevillano S, Kinsella S, Evandy C, van den Brink MR, Lemarquis A, Dudakov JA (2025 Sep 11). Damage-induced IL-18 stimulates thymic NK cells limiting endogenous tissue regeneration Nature Immunology, 26(10), 1699-1711. . PMCID: 12479347.
4.) Ju X, Hannon WW, Kaszuba T, Radford CE, Larsen BB, Nelson SS, Nelson CA, Baltazar-Perez I, Zimmerman O, Fremont DH, Diamond MS, Bloom JD (2025 Aug 25). Determinants of human versus mosquito cell entry by the Chikungunya virus envelope proteins bioRxiv, (), 2025.08.25.672233. . PMCID: 12407778.
5.) Dadonaite B, Harari S, Larsen BB, Kampman L, Harteloo A, Elias-Warren A, Chu HY, Bloom JD (2025 Aug 19). Spike mutations that affect the function and antigenicity of recent KP.3.1.1-like SARS-CoV-2 variants bioRxiv, (), 2025.08.18.671001. . PMCID: 12393327.
6.) Repele A, Pande D, Enstrom MR, Perez AM, Cui M, Madhu R, Nelson V, Kiem HP, Radtke S (2025 Aug 13). GroβT rapidly and reliably mobilizes primitive hematopoietic stem and progenitor cells in nonhuman primates Molecular Therapy. Methods & Clinical Development, 33(3), 101558. . PMCID: 12418998.
7.) Radtke S, Fields E, Swing K, Kanestrom G, Yen JS, Pande D, Enstrom MR, Humbert O, Weiss MJ, Liu DR, Newby GA, Kiem HP (2025 Aug 13). Engraftment and persistence of HBB-base-edited hematopoietic stem cells in nonhuman primates Science translational medicine, 17(811), eadn2601. . PMCID: 12490786.
8.) Wilcox-King A, Wan YH, Scharffenberger SC, Chhan CB, Davis AR, Homad LJ, Seydoux E, MacPhee KJ, Siddaramaiah LK, Melo M, Dosenovic P, Irvine DJ, Hyrien O, Stamatatos L, McGuire AT (2025 Aug 5). Priming VRC01-precursor B cells with non-envelope immunogens disfavors boosting with HIV-1 envelope NPJ Vaccines, 10(), 185. . PMCID: 12325944.
9.) Choo S, Tong AH, Fields E, Mack HM, Humbert O, Radtke S, Kiem HP (2025 Jul 17). A rescue fanconi anemia humanized mouse model with endogenous FA mutation and high human hematopoietic stem cell chimerism Molecular Therapy. Methods & Clinical Development, 33(3), 101528. . PMCID: 12355547.
10.) Paatela EM, St. Amant FG, Hamm DC, Bennett SR, Gujral TS, van der Maarel SM, Tapscott SJ (2025 Jul 8). A discrete region of the D4Z4 is sufficient to initiate epigenetic silencing Human Molecular Genetics, 34(18), 1526-1540. . PMCID: 12409625.
11.) Simonich CA, McMahon TE, Ju X, Yu TC, Brunette N, Stevens-Ayers T, Boeckh MJ, King NP, Greninger AL, Bloom JD (2025 Jul 3). RSV F evolution escapes some monoclonal antibodies but does not strongly erode neutralization by human polyclonal sera Journal of Virology, 99(7), e00531-25. . PMCID: 12282093.
12.) Simon S, Bugos G, Prins R, Rajan A, Palani A, Heyer K, Stevens A, Zeng L, Thompson KA, Atilla PA, Price JP, Kluesner MG, Jaeger-Ruckstuhl CA, Shabaneh TB, Olson JM, Su X, Riddell SR (2025 Mar 17). Design of sensitive monospecific and bispecific synthetic chimeric T cell receptors for cancer therapy Nature Cancer, 6(4), 647-665. . PMCID: 12037409.
13.) Simonich CA, McMahon TA, Ju X, Yu TC, Brunette N, Stevens-Ayers T, Boeckh MJ, King NP, Greninger AL, Bloom JD (2025 Mar 11). RSV F evolution escapes some monoclonal antibodies but does not strongly erode neutralization by human polyclonal sera bioRxiv, (), 2025.03.11.642476. . PMCID: 11952455.
14.) Petty NE, Radtke S, Kanestrom G, Fields E, Humbert O, Fiorenza S, Llewellyn MJ, Laszlo GS, Thomas J, Burger Z, Swing K, Zhu H, Jerome KR, Turtle CJ, Walter RB, Kiem HP (2025 Feb 12). Protection of CD33-modified hematopoietic stem cell progeny from CD33-directed CAR T cells in rhesus macaques Blood Advances, 9(10), 2367-2378. . PMCID: 12141896.
15.) Radford CE, Bloom JD (2025 Jan 30). Comprehensive maps of escape mutations from antibodies 10-1074 and 3BNC117 for Envs from two divergent HIV strains bioRxiv, (), 2025.01.30.635715. . PMCID: 11838258.
16.) Aditham AK, Radford CE, Carr CR, Jasti N, King NP, Bloom JD (2024 Dec 17). Deep mutational scanning of rabies glycoprotein defines mutational constraint and antibody-escape mutations bioRxiv, (), 2024.12.17.628970. . PMCID: 11702696.
17.) Cucinotta C, Dell R, Alavattam K, Tsukiyama T (2024 Dec 12). Sir2 is required for the quiescence-specific condensed three-dimensional chromatin structure of rDNA bioRxiv, (), 2024.12.12.628092. . PMCID: 11661206.
18.) Scharffenberger SC, Wan YH, Homad LJ, Kher G, Haynes AM, Poudel B, Sinha IR, Aldridge N, Pai A, Bibby M, Chhan CB, Davis AR, Moodie Z, Palacio MB, Escolano A, McElrath MJ, Boonyaratanakornkit J, Pancera M, McGuire AT (2024 Oct 22). Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies. Cell reports, 43(10), 114811. . PMID: 39383036.
19.) Carr CR, Crawford KHD, Murphy M, Galloway JG, Haddox HK, Matsen FA 4th, Andersen KG, King NP, Bloom JD (2024 Sep 10). Deep mutational scanning reveals functional constraints and antibody-escape potential of Lassa virus glycoprotein complex. Immunity, 57(9), 2061-2076.e11. . PMID: 39013466.
20.) Dadonaite B, Brown J, McMahon TE, Farrell AG, Figgins MD, Asarnow D, Stewart C, Lee J, Logue J, Bedford T, Murrell B, Chu HY, Veesler D, Bloom JD (2024 Jul 3). Spike deep mutational scanning helps predict success of SARS-CoV-2 clades Nature, 631(8021), 617-626. . PMCID: 11254757.
21.) Konecny AJ, Mage PL, Tyznik AJ, Prlic M, Mair F (2024 Jun). OMIP-102: 50-color phenotyping of the human immune system with in-depth assessment of T cells and dendritic cells. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 105(6), 430-436. . PMID: 38634730.
22.) Belmont L, Contreras M, Cartwright-Acar CH, Marceau CD, Agrawal A, Levoir LM, Lubow J, Goo L (2024 Apr 27). Functional genomics screens reveal a role for TBC1D24 and SV2B in antibody-dependent enhancement of dengue virus infection bioRxiv, (), 2024.04.26.591029. . PMCID: 11071485.
23.) Simon S, Bugos G, Prins R, Rajan A, Palani A, Heyer K, Stevens A, Zeng L, Thompson K, Price JP, Kluesner MK, Jaeger-Ruckstuhl C, Shabaneh TB, Olson JM, Su X, Riddell SR (2024 Apr 22). Sensitive bispecific chimeric T cell receptors for cancer therapy Research Square, (), rs.3.rs-4253777. . PMCID: 11092799.
24.) Larsen BB, McMahon T, Brown JT, Wang Z, Radford CE, Crowe JE Jr, Veesler D, Bloom JD (2024 Apr 19). Functional and antigenic landscape of the Nipah virus receptor binding protein bioRxiv, (), 2024.04.17.589977. . PMCID: 11042328.
25.) Carr CR, Crawford KH, Murphy M, Galloway JG, Haddox HK, Matsen FA IV, Andersen KG, King NP, Bloom JD (2024 Feb 6). Deep mutational scanning reveals functional constraints and antigenic variability of Lassa virus glycoprotein complex bioRxiv, (), 2024.02.05.579020. . PMCID: 10871245.
26.) Tischler JD, Tsuchida H, Bosire R, Oda TT, Park A, Adeyemi RO (2024 Jan 29). FLIP(C1orf112)-FIGNL1 complex regulates RAD51 chromatin association to promote viability after replication stress Nature Communications, 15(), 866. . PMCID: 10825145.
27.) Konecny AJ, Mage P, Tyznik AJ, Prlic M, Mair F (2023 Dec 15). 50-color phenotyping of the human immune system with in-depth assessment of T cells and dendritic cells bioRxiv, (), 2023.12.14.571745. . PMCID: 10760076.
28.) Dadonaite B, Brown J, McMahon TE, Farrell AG, Asarnow D, Stewart C, Logue J, Murrell B, Chu HY, Veesler D, Bloom JD (2023 Nov 14). Full-spike deep mutational scanning helps predict the evolutionary success of SARS-CoV-2 clades bioRxiv, (), 2023.11.13.566961. . PMCID: 10680755.
29.) Lubow J, Levoir LM, Ralph DK, Belmont L, Contreras M, Cartwright-Acar CH, Kikawa C, Kannan S, Davidson E, Duran V, Rebellon-Sanchez DE, Sanz AM, Rosso F, Doranz BJ, Einav S, Matsen IV FA, Goo L (2023 Oct 9). Single B cell transcriptomics identifies multiple isotypes of broadly neutralizing antibodies against flaviviruses PLOS Pathogens, 19(10), e1011722. . PMCID: 10586629.
30.) Hamm DC, Paatela EM, Bennett SR, Wong CJ, Campbell AE, Wladyka CL, Smith AA, Jagannathan S, Hsieh AC, Tapscott SJ (2023 Sep 25). The transcription factor DUX4 orchestrates translational reprogramming by broadly suppressing translation efficiency and promoting expression of DUX4-induced mRNAs PLOS Biology, 21(9), e3002317. . PMCID: 10553841.
31.) Tischler JD, Tsuchida H, Oda TT, Park A, Adeyemi RO (2023 Sep 25). RADIF(C1orf112)-FIGNL1 Complex Regulates RAD51 Chromatin Association to Promote Viability After Replication Stress. bioRxiv : the preprint server for biology, (), . . PMID: 37808755.
32.) Garcia NM, Becerra JN, McKinney BJ, DiMarco AV, Wu F, Fitzgibbon M, Alvarez JV (2023 Jul 3). APOBEC3 activity promotes the survival and evolution of drug-tolerant persister cells during acquired resistance to EGFR inhibitors in lung cancer bioRxiv, (), 2023.07.02.547443. . PMCID: 10350004.
33.) Radford CE, Schommers P, Gieselmann L, Crawford KH, Dadonaite B, Yu TC, Dingens AS, Overbaugh J, Klein F, Bloom JD (2023 Mar 24). Mapping the neutralizing specificity of human anti-HIV serum by deep mutational scanning bioRxiv, (), 2023.03.23.533993. . PMCID: 10055425.
Includes services from sterile cell sorting for therapeutic applications to whole cell mass spectrometry, diagnostics and early stage clinical trials. Provides expertise from experimental design to data analysis and troubleshooting, therapeutic cell sorting. Supports isolation of cells for Phase 1 clinical trials of cellular immunotherapies.
