Fred Hutch Cancer Center

Contact Info:

Shared Resources Administration

1100 Fairview Ave N.

DE-360

Seattle, WA 98109

https://www.fredhutch.org/en/research/shared-resources/core-facilities/experimental-histopathology.html

Grants and Identifiers:

RRID: RRID:SCR_022612

P30CA015704

Instrumentation:

Services Provided:

H&E Staining

Immunohistochemistry

In Situ Hybridization

Microtomy/Light

Multiplex Fluorescence

Sample Preparation

Special Stains Histology

Tissue Embed

Tissue MicroArray

Training

Relevant Publications:

1.) Semenova G, Frank S, Dumpit R, Han W, Coleman I, Gulati R, Morrissey C, Haffner MC, Nelson PS, Lee JK (2025 Sep 11). Genotoxic antibody-drug conjugates combined with Bcl-xL inhibitors enhance therapeutic efficacy in metastatic castration-resistant prostate cancer bioRxiv, (), 2025.09.05.674562. . PMCID: 12439934.

2.) Granadier D, Cooper K, Acenas D II, Kousa A, Warren M, Hernandez V, Iovino L, deRoos P, Lederer EE, Shannon-Sevillano S, Kinsella S, Evandy C, van den Brink MR, Lemarquis A, Dudakov JA (2025 Sep 11). Damage-induced IL-18 stimulates thymic NK cells limiting endogenous tissue regeneration Nature Immunology, 26(10), 1699-1711. . PMCID: 12479347.

3.) Gregorio NE, Zhang F, Suita Y, Ang LS, Prado O, Fasuyi A, Olson JM, Stevens KR, DeForest CA (2025 Aug 27). PhoCoil: A photodegradable and injectable single-component recombinant protein hydrogel for minimally invasive delivery and degradation Science Advances, 11(35), eadx3472. . PMCID: 12383250.

4.) Figueiredo JC, Redwood D, Li L, Donato E, Fort D, Fox EE, Grady WM, Green H, Harrison TA, Haupt C, Hsu L, Hullar MA, Huyghe JR, Johnson W, Koehne AL, LaBrie SD, Lakey MA, Lin M, Loroña NC, Maresh GA, Matrana M, Mizrahi JD, Nash SH, Nguyen NT, Paruch JL, Phipps AI, Qu C, Randolph TW, Romo S, Thomas CE, Thomas S, Tiesinga J, Whitlow C, Yeung CC, Yin H, Zibilich CM, Li CI, Thomas TK, Peters U (2025 Jun 27). The Translational Research Program in Cancer Differences across Populations Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, (), 10.1158/1055-9965.EPI-24-1711. . PMCID: 12349377.

5.) Vick SC, Domenjo-Vila E, Frutoso M, Glabman RA, Warrier LS, Hughes SM, Kirby AC, Fialkow MF, Hladik F, Prlic M, Lund JM (2025 Apr 9). Mucosal tissue NK cells tune their function between optimal anti-pathogen activity and tissue protection bioRxiv, (), 2025.04.04.647286. . PMCID: 12026740.

6.) Yamamoto N, Dobersch S, Loveless I, Samraj AN, Jang GH, Haraguchi M, Kang LI, Ruzinova MB, Vij KR, Mudd JL, Walsh T, Safyan RA, Chiorean EG, Hingorani SR, Bolton NM, Li L, Fields RC, DeNardo DG, Notta F, Crawford HC, Steele NG, Kugel S (2025 Feb 17). HMGA2 Expression Predicts Subtype, Survival, and Treatment Outcome in Pancreatic Ductal Adenocarcinoma Clinical cancer research : an official journal of the American Association for Cancer Research, 31(4), 733-745. . PMCID: 11967372.

7.) Rominger MC, Gupta S, Moorthi S, McSharry M, Kamlapurkar S, O’Brien S, Waldum A, Lo A, Duke F, Lowe AR, Cromwell E, Glabman R, Koehne A, Berger AH (2024 Nov 12). Mutant RIT1 cooperates with YAP to drive an EMT-like lung cancer state bioRxiv, (), 2024.11.11.623044. . PMCID: 11601272.

8.) Graham JB, Swarts JL, Koehne AL, Watson CE, Lund JM (2024 Oct). Regulatory T cells restrict immunity and pathology in distal tissue sites following a localized infection. Mucosal immunology, 17(5), 923-938. . PMID: 38908483.

9.) Garcia NM, Becerra JN, McKinney BJ, DiMarco AV, Wu F, Fitzgibbon M, Alvarez JV (2023 Jul 3). APOBEC3 activity promotes the survival and evolution of drug-tolerant persister cells during acquired resistance to EGFR inhibitors in lung cancer bioRxiv, (), 2023.07.02.547443. . PMCID: 10350004.

Description:

Provides expertise in anatomic pathology technologies. Offers routine and special staining,  immunohistochemistry, in situ hybridization and digital pathology in support of internal and external investigators who rely on histological analysis.