Fred Hutch Cancer Center
Shared Resources Administration
1100 Fairview Ave N.
DE-780
Seattle, WA 98109
https://www.fredhutch.org/en/research/shared-resources/core-facilities/electron-microscopy.html
RRID: RRID:SCR_022611
ThermoFisher Talos L120C TEM
Leica EMUC7
Leica VT1200S
Leica EMPact2
Denton Benchtop Turbo Coating System
Beckman Airfuge
JEOL JSM 6610LV SEM
Tousimis Critical Point Dryer
Denton Desk IV Sputter Coater
ThermoFisher Scientific IV Vitrobot
Talos L120C TEM
200kV Glacios TEM and Gatan K3 Direct Electron Detector
Cryo-Electron Microscopy
Electron Microscopy
Scanning Electron Microscopy
Training
1.) Carter JJ, Hurlburt NK, Scherer EM, Singh S, Rodarte JV, Smith RA, Lewis P, Kinzelman R, Kieltyka J, Cabãn ME, Wipf GC, Pancera M, Galloway DA (2025 Oct 21). Human monoclonal antibodies to HPV16 show evidence for common developmental pathways and public epitopes PLOS Pathogens, 21(10), e1013086. . PMCID: 12551957.
2.) Kelnhofer-Millevolte LE, Smith JR, Nguyen DH, Wilson LS, Lewis HC, Arnold EA, Brinkley MR, Shin K, Ahn JH, Kim ET, Kulej K, Geballe AP, Ramachandran S, Avgousti DC (2025 Aug 8). Human cytomegalovirus induces neuronal gene expression through IE1 for viral maturation Nature Communications, 16(), 7316. . PMCID: 12334593.
3.) Wang X, Guillem‐Marti J, Kumar S, Lee DS, Cabrerizo‐Aguado D, Werther R, Alamo KA, Zhao YT, Nguyen A, Kopyeva I, Huang B, Li J, Hao Y, Li X, Brizuela‐Velasco A, Murray A, Gerben S, Roy A, DeForest CA, Springer T, Ruohola‐Baker H, Cooper JA, Campbell MG, Manero JM, Ginebra M, Baker D (2025 Jun 9). De Novo Design of Integrin α5β1 Modulating Proteins to Enhance Biomaterial Properties Advanced Materials (Deerfield Beach, Fla.), 37(34), 2500872. . PMCID: 12392869.
4.) Readshaw JJ, Doyle LA, Puiu M, Kelly A, Nelson A, Kaiser AJ, McGuire S, Peralta-Acosta J, Smith DL, Stoddard BL, Kaiser BK, Blower TR (2025 Mar 26). PglZ from Type I BREX phage defence systems is a metal-dependent nuclease that forms a sub-complex with BrxB bioRxiv, (), 2025.03.26.645558. . PMCID: 11974810.
5.) Gupta A, Morella N, Sutormin D, Li N, Gaisser K, Robertson A, Ispolatov Y, Seelig G, Dey N, Kuchina A (2025 Jan 14). Combinatorial phenotypic landscape enables bacterial resistance to phage infection bioRxiv, (), 2025.01.13.632860. . PMCID: 11761130.
6.) Scharffenberger SC, Wan YH, Homad LJ, Kher G, Haynes AM, Poudel B, Sinha IR, Aldridge N, Pai A, Bibby M, Chhan CB, Davis AR, Moodie Z, Palacio MB, Escolano A, McElrath MJ, Boonyaratanakornkit J, Pancera M, McGuire AT (2024 Oct 22). Targeting RSV-neutralizing B cell receptors with anti-idiotypic antibodies. Cell reports, 43(10), 114811. . PMID: 39383036.
7.) Wang X, Guillem-Marti J, Kumar S, Lee DS, Cabrerizo-Aguado D, Werther R, Alamo KA, Zhao YT, Nguyen A, Kopyeva I, Huang B, Li J, Hao Y, Li X, Brizuela-Velasco A, Murray A, Gerben S, Roy A, DeForest CA, Springer T, Ruohola-Baker H, Cooper JA, Campbell MG, Manero JM, Ginebra MP, Baker D (2024 Jul 10). De Novo Design of Integrin α5β1 Modulating Proteins for Regenerative Medicine bioRxiv, (), 2024.06.21.600123. . PMCID: 11230231.
8.) Hansman GS, Kher G, Svirina AD, Tame JR, Hartley-Tassell L, Irie H, Haselhorst T, von Itzstein M, Rudd PA, Pancera M (2024 Jul 2). Development of a broad-spectrum therapeutic Fc-nanobody for human noroviruses Journal of Virology, 98(7), e00707-24. . PMCID: 11264634.
9.) Kelnhofer-Millevolte LE, Smith JR, Nguyen DH, Wilson LS, Lewis HC, Arnold EA, Brinkley MR, Geballe AP, Ramachandran S, Avgousti DC (2024 Jun 13). Human cytomegalovirus induces neuronal gene expression for viral maturation bioRxiv, (), 2024.06.13.598910. . PMCID: 11195207.
Offers techniques to resolve structures from cellular ultrastructure to molecular interactions. Offers expertise in EM sample preparation and imaging, cryogenic electron microscopy, technologies and services, training through complexities of electron microscopy workflow, from sample preparation to image processing. All users must be trained by core staff.
