Fred Hutch Cancer Center
Shared Resources Administration
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Seattle, WA 98109
https://www.fredhutch.org/en/research/shared-resources/core-facilities/cellular-imaging.html
RRID: RRID:SCR_022609
Other Citation IdentifiersP30CA015704
Nikon Eclipse E800
DeltaVision Elite
Nikon Live
Nikon TIRF/STORM
Incucyte S3
Incucyte Zoom
TissueFAXS
Molecular Devices ImageXpress
Leica SP8
Zeiss 780
LaVision Ultramicroscope II
GE Typhoon Trio
Li-cor Odyssey
Analysis Workstations
Cell Imaging
Confocal Microscopy
Image Processing And Analysis
Microscopy
1.) Harris ED, McGovern M, Pernikoff S, Ikeda R, Kipnis L, Hannon W, Sobolik EB, Gray M, Greninger AL, He S, Chin CN, Fu TM, Pancera M, Boonyaratanakornkit J (2025 Jul 20). DEVELOPMENT OF A POTENT MONOCLONAL ANTIBODY FOR TREATMENT OF HUMAN METAPNEUMOVIRUS INFECTIONS bioRxiv, (), 2025.06.09.657676. . PMCID: 12262254.
2.) MacLean F, Zemek RM, Tsegaye AT, Graham JB, Swarts JL, Vick SC, Potchen NB, Talavera IC, Warrier L, Dubrulle J, Schroeder LK, Elz A, Sowerby D, Saito A, Thomas KK, Mack M, Schiffer JT, McClelland RS, Jerome KR, Chohan BH, Ngure K, Mugo NR, Newell EW, Lingappa JR, Lund JM (2025 Jun 27). Genital herpes shedding episodes associate with alterations in the spatial organization and activation of mucosal immune cells bioRxiv, (), 2025.06.24.661157. . PMCID: 12262732.
3.) Kumar S, Jiang J, Donald-Paladino MS, Chen J, Gutierrez A, Federation AJ, Szulzewsky F, Holland EC, Ferguson FM, Nabet B (2025 Jun 24). Development of PROTACs for targeted degradation of oncogenic TRK fusions bioRxiv, (), 2025.06.18.660465. . PMCID: 12262543.
4.) Alassaf M, Madan A, Ranganathan S, Marschall S, Wong JJ, Goldberg ZH, Brent AE, Rajan A (2025 May 14). Adipocyte metabolic state regulates glial phagocytic function Cell reports, 44(5), 115704. . PMCID: 12169346.
5.) Ishida T, Mercoli J, Heck AM, Phelps I, Varnum-Finney B, Dozono S, Nourigat-McKay C, Kraskouskas K, Wellington R, Waltner O, Jackson DL, Delaney C, Rafii S, Bernstein ID, Aldinger KA, Trapnell C, Zhao HG, Hadland B (2025 May 2). Differentiation latency, cell division symmetry, and dormancy signatures define fetal liver HSCs at single cell resolution bioRxiv, (), 2023.06.01.543314. . PMCID: 10274697.
6.) Riddler SA, Moodie Z, Clark J, Yen C, Allen M, Furch BD, Lu H, Grant S, Mondal K, Anderson M, Maenza J, Lemos MP, Woodward Davis AS, Walsh SR, Sobieszczyk ME, Frank I, Goepfert P, Stephenson KE, Baden LR, Tieu HV, Keefer MC, McElrath MJ, Kublin JG, Corey L (2025 Apr 29). High Frequency of Chronic Urticaria Following an Investigational HIV-1 BG505 MD39.3 Trimer mRNA Vaccine in a Phase 1, Randomized, Open-Label Clinical Trial (HVTN 302) Annals of internal medicine, 178(7), 963-974. . PMCID: 12404031.
7.) MacLean F, Tsegaye AT, Graham JB, Swarts JL, Vick SC, Potchen NB, Cruz Talavera I, Warrier L, Dubrulle J, Schroeder LK, Saito A, Mar C, Thomas KK, Mack M, Sabo MC, Chohan BH, Ngure K, Mugo NR, Lingappa JR, Lund JM (2025 Mar 25). Bacterial vaginosis associates with dysfunctional T cells and altered soluble immune factors in the cervicovaginal tract The Journal of Clinical Investigation, 135(10), e184609. . PMCID: 12077898.
8.) Simon S, Bugos G, Prins R, Rajan A, Palani A, Heyer K, Stevens A, Zeng L, Thompson KA, Atilla PA, Price JP, Kluesner MG, Jaeger-Ruckstuhl CA, Shabaneh TB, Olson JM, Su X, Riddell SR (2025 Mar 17). Design of sensitive monospecific and bispecific synthetic chimeric T cell receptors for cancer therapy Nature Cancer, 6(4), 647-665. . PMCID: 12037409.
9.) Wang MF, Li MY, Yang YC, Chuang YC, Tsai CY, Binder MC, Ma L, Lin SW, Li HW, Smith GR, Chi P (2025 Mar 4). Mug20–Rec25–Rec27 binds DNA and enhances meiotic DNA break formation via phase-separated condensates Nucleic Acids Research, 53(5), gkaf123. . PMCID: 11879393.
10.) Li D, Geng K, Hao Y, Gu J, Kumar S, Olson AT, Kuismi CC, Kim HM, Pan Y, Sherman F, Williams AM, Li Y, Li F, Chen T, Thakurdin C, Ranieri M, Meynardie M, Levin DS, Stephens J, Chafitz A, Chen J, Donald-Paladino MS, Powell JM, Zhang ZY, Chen W, Ploszaj M, Han H, Gu SS, Zhang T, Hu B, Nacev BA, Kaiza ME, Berger AH, Wang X, Li J, Sun X, Liu Y, Zhang X, Bruno TC, Gray NS, Nabet B, Wong KK, Zhang H (2024 Dec 24). Targeted degradation of oncogenic KRASG12V triggers antitumor immunity in lung cancer models The Journal of Clinical Investigation, 135(2), e174249. . PMCID: 11735103.
11.) Arends T, Bennett SR, Tapscott SJ (2024 Dec 18). DUX4-induced HSATII RNA accumulation drives protein aggregation impacting RNA processing pathways bioRxiv, (), 2024.12.17.628988. . PMCID: 11702838.
12.) Cucinotta C, Dell R, Alavattam K, Tsukiyama T (2024 Dec 12). Sir2 is required for the quiescence-specific condensed three-dimensional chromatin structure of rDNA bioRxiv, (), 2024.12.12.628092. . PMCID: 11661206.
13.) Doedens JR, Diamond C, Harrison D, Bock MG, Clarke N, Watt AP, Gabel CA (2024 Sep). The ester-containing prodrug NT-0796 enhances delivery of the NLRP3 inflammasome inhibitor NDT-19795 to monocytic cells expressing carboxylesterase-1. Biochemical pharmacology, 227(), 116455. doi: 10.1016/j.bcp.2024.116455. PMID: 39069136.
14.) MacLean F, Tsegaye AT, Graham JB, Swarts JL, Vick SC, Potchen N, Talavera IC, Warrier L, Dubrulle J, Schroeder LK, Mar C, Thomas KK, Mack M, Sabo MC, Chohan BH, Ngure K, Mugo N, Lingappa JR, Lund JM (2024 Jul 5). Bacterial vaginosis-driven changes in vaginal T cell phenotypes and their implications for HIV susceptibility bioRxiv, (), 2024.07.03.601916. . PMCID: 11245000.
15.) Stjepić V, Nakamura M, Hui J, Parkhurst SM (2024 May 28). Two Septin complexes mediate actin dynamics during cell wound repair. Cell reports, 43(5), 114215. . PMID: 38728140.
16.) Simon S, Bugos G, Prins R, Rajan A, Palani A, Heyer K, Stevens A, Zeng L, Thompson K, Price JP, Kluesner MK, Jaeger-Ruckstuhl C, Shabaneh TB, Olson JM, Su X, Riddell SR (2024 Apr 22). Sensitive bispecific chimeric T cell receptors for cancer therapy Research Square, (), rs.3.rs-4253777. . PMCID: 11092799.
17.) Doedens JR, Smolak P, Nguyen M, Wescott H, Diamond C, Schooley K, Billinton A, Harrison D, Koller BH, Watt AP, Gabel CA (2024 Apr 18). Pharmacological Analysis of NLRP3 Inflammasome Inhibitor Sodium [(1,2,3,5,6,7-Hexahydro-s-indacen-4-yl)carbamoyl][(1-methyl-1H-pyrazol-4-yl)({[(2S)-oxolan-2-yl]methyl})sulfamoyl]azanide in Cellular and Mouse Models of Inflammation Provides a Translational Framework ACS Pharmacology & Translational Science, 7(5), 1438-1456. . PMCID: 11091978.
18.) Rupert PB, Buerger M, Girard EJ, Frutoso M, Parrilla D, Ng K, Gooley T, Groh V, Strong RK (2024 Mar 27). Preclinical characterization of Pan-NKG2D ligand-binding NKG2D receptor decoys Heliyon, 10(7), e28583. . PMCID: 10998067.
19.) Arends T, Tsuchida H, Adeyemi RO, Tapscott SJ (2024 Mar 7). DUX4-induced HSATII transcription causes KDM2A/B–PRC1 nuclear foci and impairs DNA damage response The Journal of Cell Biology, 223(5), e202303141. . PMCID: 10919155.
20.) Jaeger-Ruckstuhl CA, Lo Y, Fulton E, Waltner OG, Shabaneh TB, Simon S, Muthuraman PV, Correnti CE, Newsom O, Engstrom IA, Kanaan SB, Bhise SS, Peralta JM, Ruff R, Price JP, Stull SM, Stevens AR, Bugos G, Kluesner MG, Voillet V, Muhunthan V, Morrish F, Olson JM, Gottardo R, Sarthy JF, Henikoff S, Sullivan LB, Furlan SN, Riddell SR (2024 Feb 13). Signaling via a CD27-TRAF2-SHP-1 axis during naïve T cell activation promotes memory-associated gene regulatory networks Immunity, 57(2), 287-302.e12. . PMCID: 10967230.
21.) Jaeger-Ruckstuhl CA, Lo Y, Fulton E, Waltner OG, Shabaneh TB, Simon S, Muthuraman PV, Correnti CE, Newsom OJ, Engstrom IA, Kanaan SB, Bhise SS, Peralta JMC, Ruff R, Price JP, Stull SM, Stevens AR, Bugos G, Kluesner MG, Voillet V, Muhunthan V, Morrish F, Olson JM, Gottardo R, Sarthy JF, Henikoff S, Sullivan LB, Furlan SN, Riddell SR (2024 Feb 13). Signaling via a CD27-TRAF2-SHP-1 axis during naive T cell activation promotes memory-associated gene regulatory networks. Immunity, 57(2), 287-302.e12. . PMID: 38354704.
22.) Tischler JD, Tsuchida H, Bosire R, Oda TT, Park A, Adeyemi RO (2024 Jan 29). FLIP(C1orf112)-FIGNL1 complex regulates RAD51 chromatin association to promote viability after replication stress Nature Communications, 15(), 866. . PMCID: 10825145.
23.) Riley AK, Grant M, Snell A, Vichas A, Moorthi S, Urisman A, Castel P, Wan L, Berger AH (2023 Dec 1). The deubiquitinase USP9X regulates RIT1 protein abundance and oncogenic phenotypes bioRxiv, (), 2023.11.30.569313. . PMCID: 10705424.
24.) Loeb AM, Pattwell SS, Meshinchi S, Bedalov A, Loeb KR (2023 Oct 10). Donor bone marrow-derived macrophage engraftment into the central nervous system of patients following allogeneic transplantation. Blood advances, 7(19), 5851-5859. . PMID: 37315172.
25.) Tischler JD, Tsuchida H, Oda TT, Park A, Adeyemi RO (2023 Sep 25). RADIF(C1orf112)-FIGNL1 Complex Regulates RAD51 Chromatin Association to Promote Viability After Replication Stress. bioRxiv : the preprint server for biology, (), . . PMID: 37808755.
26.) Davidson KA, Nakamura M, Verboon JM, Parkhurst SM (2023 Aug 7). Centralspindlin proteins Pavarotti and Tumbleweed along with WASH regulate nuclear envelope budding. The Journal of cell biology, 222(8), . . PMID: 37163553.
27.) Loeb AM, Pattwell SS, Meshinchi S, Bedalov A, Loeb KR (2023 Jun 16). Donor bone marrow–derived macrophage engraftment into the central nervous system of patients following allogeneic transplantion Blood Advances, 7(19), 5851-5859. . PMCID: 10558597.
The Cellular Imaging shared resource at Fred Hutch provides advanced light microscopy services for biomedical and translational research. Our expert staff provides a complete workflow, including training for independent, 24/7 work in the core; assistance and technical support in microscopy techniques and their application; and tailored quantitative image analysis. We also offer training and support for imaging and analyzing gels and blots. We work with Fred Hutch users as well as external users from academia and industry.
Fred Hutch and Leica Microsystems have combined efforts to establish a Leica Center of Excellence in the Cellular Imaging core in support of a mission to drive new discoveries and insights from scientific research performed using the imaging systems.
