Facility Details

The Molecular Biology Core Facilities (MBCF) at Dana-Farber Cancer Institute (DFCI) offers a full suite of services for a wide variety of genomic next-generation sequencing (NGS) library preparation, sequencing and analysis applications including RNAseq, ChIPseq, ATACseq, CRISPR screening, whole genome methylation profiling, targeted resequencing, single-cell RNAseq, exome sequencing, and more. The genomics core technology platforms include Illumina NovaSeq6000, NextSeq500s, MiSeqs, MiniSeq.

High throughput sample preparation is performed on Beckman Coulter Biomek FX and i7 systems. Low throughput precious samples are prepared by experienced technical staff.

The MBCF writes and maintains standardized pipelines for routine NGS analysis such as RNAseq, ChIPseq, and CRISPR screening. Complex bioinformatics analysis such as integration of multi-omics datasets or specialized analyses can be performed by the experienced computational biology team.

Genomics email: zachary_herbert@dfci.harvard.edu
Proteomics email: james_lee@dfci.harvard.edu
and everything: paul_morrison@dfci.harvard.edu