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Rockefeller University
1230 York Ave
Box 295
New York, NY 10065
United Stateshttps://www.rockefeller.edu/structuralbiologyDeena Oren
Last Updated: 03/03/2021
The Structural Biology Resource Center (SBRC) is home to state-of-the-art X-ray and liquid handling equipment for the determination of the three-dimensional structures of biological macromolecules. The Center houses the Rigaku/MSC microMax 007HF generator equipped with Varimax optics that can be adjusted for small samples (300 micron), X-stream cryosystems and two RaxisIV++ detectors. The center also has a UVEX (Jan Scientific) microscope for identifying protein crystals of any size and two liquid handlers, the Formulator (Formulatrix) for mixing stock solutions and the Phoenix (Art Robbins) for setting up trays.
Based on availability, scientists from external not-for-profit research institutions may access the services of the Center. Fees for non-Rockefeller use of the instrumentation are listed on our website.
Services are offerred outside of Rockefeller University
Consulting is offerred outside of Rockefeller University
General Electric AKTA FPLC System, Protein Purification System
Purification system intended for microgram-scale protein purifications in Tricorn columns up to milligram-scale using HiTrap and HiScreen columns. For fast protein liquid chromatography of tens of grams of protein,can be used with flow rates up to 150 mL/min. This high flow rate is necessary when working with high-flow resins in large columns. Columns can even be packed at flow rates as high as 300 mL/min. System reduces hands-on time so that you can spend less time purifying proteins and more time focusing on your results. Easily switch between different users and applications. To get you up and running quickly, methods are easily created by dragging and dropping predefined templates in UNICORN 7. Purification of protein to sufficiently high yield and purity can be a time- and resource-consuming process. To address fast and efficient protein purification, Can be set up in different ways to handle multistep purification. With an additional column valve up to 10 columns can be connected enabling automation of several samples and steps. [Product Link]
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HF: Raxis IV++, Varimax-HF Optics, X-stream cryosystem, on Micromax 007 HF generator
HR: Raxis IV++, Varimax-HR Optics, X-stream cryosystem, on Micromax 007 HF generator
JANSi UVEX microscope for detecting protein crystals
Nikon SMZ-18 stereomicroscope
Wyatt Dawn Heleos II SEC-MALS
NGC Quest Bio-Rad FPLC system
Hampton Research cryo-tools for crystal manipulations in liquid nitrogen
Boyd cryo-tools and pucks for preparing samples for the use of the automounting robot at synchrotrons (BNL: X29, X6A, 12B, 12C, APS: NE-CAT 24-ID-C/E, GM/CA CAT 23, ALS: 5.0.1, 5.0.2, 5.0.3, 8.2.1 and 8.2.2)
Formulator by Formulatrix liquid handler for preparing crystallization reservoirs from stock solutions, in 96- or 24-well formats, into individual crystallization plates (regular and low profile) or deep well blocks for multiple setups
Phoenix by Art Robbins nano-volume liquid handler for filling reservoirs of 96-well trays and for placement of protein + reservoir nano volumes (200 nl - 2 ul) as well as any additives, additional components or crystal seeds
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https://coremarketplace.org/?FacilityID=179Keywords:
Protein, expression, purification, 3D, structure, X-ray, crystallography
Resource Type:
Resource, service resource, core facility, access service resource
Citation:
Rockefeller University Structural Biology Resource Center Core Facility (RRID:SCR_017732)
Facility Details
About This Facility
Services and Equipment
Publications
Associations
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